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Biochem Biophys Res Commun. 2009 Jul 3. [Epub ahead of print]
Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.
Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of 5 influenza-vaccinated volunteers, with newly developed murine-human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: 1 for influenza A H1N1, 4 for influenza A H3N2 and 5 for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and 4 (2 for H3N2 and 2 for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the 2 broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.
PMID: 19580789 [PubMed - as supplied by publisher]
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Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.
Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of 5 influenza-vaccinated volunteers, with newly developed murine-human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: 1 for influenza A H1N1, 4 for influenza A H3N2 and 5 for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and 4 (2 for H3N2 and 2 for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the 2 broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.
PMID: 19580789 [PubMed - as supplied by publisher]
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