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Anal Chem. 2013 Feb 8. [Epub ahead of print]
A Simple Rapid and Point-of-Care Microfluidic Immunomagnetic Fluorescence Assay for Pathogens.
Zhang RQ, Liu SL, Zhao W, Zhang W, Yu X, Li Y, Li AJ, Pang DW, Zhang ZL.
Abstract
In this work, we reported a simple rapid and point-of-care magnetic immunofluorescence assay for avian influenza virus (AIV) and developed a portable experimental setup equipped with an optical fiber spectrometer and microfluidic device. We achieved the integration of immunomagnetic target capture, concentration, and fluorescence detection in the magnetic microfluidic chip. By optimizing flow rate and incubation time, we could get a limit of detection low up to 3.7?10(4 )copy/μL with sample consumption of 2 μL and a total assay time of less than 55 min. This approach had proved to possess high portable, fast analysis, high specificity, high precision and reproducibility with an intra-assay variability of 2.87% and an inter-assay variability of 4.36%. As a whole, this microfluidic system may provide a powerful platform for the rapid detection of AIV and may be extended for detection of other viral pathogens, and in addition this portable experimental setup enables the development of point-of-care diagnostic systems while retaining adequate sensitivity.
PMID:
23391352
[PubMed - as supplied by publisher]
A Simple Rapid and Point-of-Care Microfluidic Immunomagnetic Fluorescence Assay for Pathogens.
Zhang RQ, Liu SL, Zhao W, Zhang W, Yu X, Li Y, Li AJ, Pang DW, Zhang ZL.
Abstract
In this work, we reported a simple rapid and point-of-care magnetic immunofluorescence assay for avian influenza virus (AIV) and developed a portable experimental setup equipped with an optical fiber spectrometer and microfluidic device. We achieved the integration of immunomagnetic target capture, concentration, and fluorescence detection in the magnetic microfluidic chip. By optimizing flow rate and incubation time, we could get a limit of detection low up to 3.7?10(4 )copy/μL with sample consumption of 2 μL and a total assay time of less than 55 min. This approach had proved to possess high portable, fast analysis, high specificity, high precision and reproducibility with an intra-assay variability of 2.87% and an inter-assay variability of 4.36%. As a whole, this microfluidic system may provide a powerful platform for the rapid detection of AIV and may be extended for detection of other viral pathogens, and in addition this portable experimental setup enables the development of point-of-care diagnostic systems while retaining adequate sensitivity.
PMID:
23391352
[PubMed - as supplied by publisher]
