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  • snippets

    from emails or pubmed or such

    this is being edited and updated by editing without bumping

    ----------------------------------

    The antigenic variation of influenza virus represents a major health problem, thus continuous efforts have been made to develop broad-spectrum vaccines against influenza virus. Matrix protein 1 (M1) protein is highly conserved in all influenza A strains. In this study, M1 protein was efficiently exp …

    Chitosan as an adjuvant

    ----------------------------
    Q223R mutation greatly enhanced infectivity of HA-pseudotypes in human cells
    I notice a lot of the egg passages results in Q223 mutations.
    I often wondered if they happen very frequently without eggs?
    ---------------------------------
    3-month
    50 μg/d (2000 IU)
    25(OH)D levels increased in the active vitamin D group from a mean
    of 64.3 ± 25.4 nmol/L to 88.5 ± 23.2 nmol/L.
    did not show that vitamin D3 supplementation changed circulating cytokine
    levels among healthy adults.
    -----------------------------------
    promising practices database
    cidrappractices.org is your first and best source for all of the information you’re looking for. From general topics to more of what you would expect to find here, cidrappractices.org has it all. We hope you find what you are searching for!

    This article describes efforts to create, share, and sustain an online clearinghouse of expert-reviewed "promising practices" in pandemic influenza preparedness from September 2006 to December 2008. This project involved six activities: (1) determining focus areas, (2) defining a promising practice, …

    ------------------------------------
    region 8: NW-USA

    going up ?
    ----------------------------------
    H3N2 : 4 low reactors at GISAID, (out of ~150), no specific mutations,
    no specific strain
    ---------------------------------
    Mexico sequence from March,11 at GISAID, apparently pre-Cancun
    ---------------------------------


    Mexico's first known swine flu case was a six-month-old baby girl in a
    northern part of the country who had no known contact with pig farms,
    "It's a six-month-old baby girl from San Luis Potosi who is alive" and first
    showed symptoms of the new strain of the influenza A(H1N1) virus on
    February 24, said Celia Alpuche of the Institute of Epidemiological Diagnosis
    and Reference (INDRE) in Mexico City.

    But studies carried out on a backlog of samples show that a first handful of
    recorded cases appeared in March in central and northern Mexico, before any
    showed up further south, said Alpuche.

    "We have other positive samples in March from Baja California (northwest),
    San Luis Potosi and Mexico City (center)," Alpuche said, referring to results
    discovered around one month ago.

    "It's complicated to say where it originated but the earlier samples are
    not from rural areas, that's to say areas with farms (or) pigs," she added.

    ----------------

    they may know it, but have an interest to keep it secret.
    They presumably did check where those people -i.e. the baby -
    may have got it from
    -------------------------------
    Bodies of water could serve as a transmission pathway for AIV in waterfowl. For AIV surveillance purposes, water samples and fecal samples appear to be excellent alternatives or additions to cloacal and oropharyngeal swabbing. Furthermore, duck age (even within hatch-year birds) may be important whe …

    H4N6 in mallards:
    Fecal samples had significantly higher virus concentrations than oropharyngeal or
    cloacal swabs and 6 month old ducks shed significantly more viral RNA than 3
    month old ducks regardless of sample type. Use of a water source contaminated
    by AIV infected mallards, was sufficient to transmit virus to naïve mallards,
    which shed AIV at higher or similar levels as orally-inoculated ducks.
    -------
    now try that for chicken viruses like H5N1 or H9N2
    -------------------------------------------
    I'm interested in expert panflu damage estimates
    my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

  • #2
    Re: snippets

    From our email exchange

    gs:
    selected North American avian amino-acid sequences , segments 1,2,3,5,7,8
    close to the bird index over long time spans

    as you can see it doesn't accumulate protein differences in these even over 30 years !

    first column: difference in promille,
    second column: number of protein-differences
    3rd column : number of amino-acids considered

    1
    0 0 760 15 >A/turkey/CO/118899/1972(H5N2),1972//
    0 0 760 30 >A/mallard Dk/ALB/57/1976(H5N2),1976/08/12
    0 0 760 111 >A/Gs/MN/5733-1/1980(H9N2),1980/01/01
    0 0 760 272 >A/mallard/Ohio/424/1988(H3N2),1988//
    0 0 760 806 >A/blue-winged teal/Texas/82/01(H2N1),2001//
    0 0 760 1131 >A/green-winged teal/Ohio/1292/05(H4N6),2005//
    0 0 756 2138 >A/blue-winged teal/Minnesota/Sg-00450/08(H4N8),2008/08/20

    2
    0 0 758 41 >A/mallard Dk/ALB/224/1977(H7N5),1977/08/05
    0 0 758 123 >A/mallard Dk/ALB/645/1980(H5N2),1980/08/22
    0 0 758 202 >A/mallard Dk/ALB/438/1985(H3N4),1985/08/20
    0 0 758 355 >A/mallard/Alberta/11/1991(H9N2),1991/08/09
    0 0 758 449 >A/turkey/Utah/24721-10/1995(H7N3),1995//
    0 0 758 644 >A/mallard/Minnesota/192/99(H4N6),1999//
    0 0 758 1110 >A/mallard/Alberta/154/03(H6N5),2003/08/03
    0 0 758 2002 >A/American wigeon/Minnesota/Sg-00231/07(H4N6),2007/09/29

    3*
    0 0 717 501 >A/mallard/ALB/196/96(H10N7),1996/08/
    0 0 717 696 >A/mallard/Minnesota/410/00(H5N2),2000//
    0 0 717 1130 >A/ruddy turnstone/New Jersey/26/04(H10N7),2004//
    0 0 717 2182 >A/mallard/Minnesota/Sg-00672/08(H3N8),2008/08/03
    13 1 717 45 >A/canvasback Dk/ALB/274/1977(H4N6),1977/08/09
    13 1 717 175 >A/mallard Dk/Tennessee/11464/1985(H1N1),1985/01/16
    13 1 717 329 >A/blue-winged teal/ALB/136/1990(H4N3),1990/08/22
    13 1 717 555 >A/mallard/Minnesota/95/98(H4N2),1998//

    3
    13 1 717 43 >A/mallard Dk/ALB/46/1977(H1N1),1977/08/08
    13 1 717 90 >A/mallard Dk/ALB/106/1979(H4N2),1979/08/07
    27 2 715 234 >A/green-winged teal/Ohio/466/1987(H3N8),1987//
    27 2 716 12 >A/turkey/Oregon/1971(H7N3),1971//
    27 2 717 39 >A/mallard Dk/ALB/42/1977(H1N6),1977/08/02
    27 2 717 156 >A/redhead Dk/ALB/357/1983(H11N9),1983/08/07
    27 2 717 246 >A/green-winged teal/Louisiana/213GW/1987(H1N1),1987//
    27 2 717 329 >A/blue-winged teal/ALB/136/1990(H4N3),1990/08/22
    27 2 717 376 >A/pintail/Alberta/129/1993(H10N7),1993/12/01
    27 2 717 919 >A/ruddy turnstone/Delaware/262/02(H2N1),2002//
    27 2 717 1587 >A/glaucous gull/Alaska/44199-097/06(H13N3),2006/09/25
    27 2 717 1809 >A/N.shoveler/Alaska/7MP0954/07(H4N6),2007/09/06

    5
    0 0 499 7 >A/turkey/Canada/1963(H6N8),1963//
    20 1 485 1429 >A/N.pintail/Alaska/44160-060/06(H12N5),2006/08/18
    20 1 494 258 >A/mottled Dk/LA/32M/87(H6N2),1987//
    20 1 495 548 >A/mallard/MN/284/98(H7N3),1998//
    20 1 499 17 >A/Dk/Pennsylvania/1/1969(H6N1),1969//
    20 1 499 23 >A/Dk/Memphis/928/1974(H3N8),1974//
    20 1 499 71 >A/pintail Dk/ALB/303/1977(H10N7),1977/09/03
    20 1 499 108 >A/pintail Dk/ALB/462/1979(H3N6),1979/08/10
    20 1 499 164 >A/Dk/Washington/4954/1983(H3N8),1983//
    20 1 499 267 >A/Dk/Louisiana/188D/1987(H12N5),1987//
    20 1 499 382 >A/mallard/ALB/199/1992(H6N5),1992/12/01
    20 1 499 483 >A/mallard/ALB/201/96(H2N3),1996/08/
    20 1 499 671 >A/red knot/Delaware/1269/00(H10N7),2000//
    20 1 499 1020 >A/blue-winged teal/Ohio/658/04(H7N3),2004//
    20 1 499 1748 >A/N.shoveler/CA/HKWF1325/07(H8N4),2007/12/09

    7
    0 0 317 2096 >A/mallard/Minnesota/Sg-00170/07(H6N1),2007/09/16
    0 0 322 724 >A/herring gull/Delaware/281/98(H5N8),1998//
    0 0 323 549 >A/Dk/Pennsylvania/Sg-00269/1995(H2N3),1995//
    0 0 324 725 >A/shorebird/Delaware/27/98(H5N8),1998//
    0 0 339 314 >A/red knot/Delaware/2561/87(H10N5),1987//
    0 0 344 640 >A/mallard/Alberta/226/98(H2N3),1998//
    0 0 348 12 >A/Turkey/CA/189/66(H9N2),1966//
    0 0 348 457 >A/Qa/Arkansas/29209-1/93(H9N2),1993//
    0 0 348 1257 >A/mallard/Ohio/671/02(H4N6),2002/08/19
    0 0 349 351 >A/mallard/Ohio/298/1987(H4N6),1987/10/19
    0 0 350 159 >A/domestic Dk/MN/1086/1980(H4N8),1980/09/29
    0 0 350 197 >A/pintail Dk/NY/155/1982(H4N3),1982/12/01
    0 0 350 357 >A/mallard/Alberta/321/88(H9N2),1988//
    0 0 351 4 >A/turkey/Canada/1963(H6N8),1963//
    0 0 351 16 >A/Turkey/Oregon/71(H7N3NSB),1971//
    0 0 351 24 >A/blue Gs/WI/711/1975(H5N2),1975//
    0 0 351 26 >A/Canada Gs/Wisconsin/902/1975(H5N2),1975//
    0 0 351 36 >A/mallard Dk/ALB/25/1976(H3N1),1976/08/11
    0 0 351 178 >A/turkey/Minnesota/1598/1981(H5N2),1981/11/06

    8
    0 0 320 398 >A/mallard/Alberta/11/91(H9N2),1991//
    0 0 331 666 >A/mallard/Alberta/111/99(H4N6),1999//
    0 0 353 31 >A/mallard/Wisconsin/34/1975(H5N6),1975//
    0 0 353 62 >A/mallard Dk/ALB/46/1977(H1N1),1977/08/08
    0 0 353 126 >A/pintail Dk/ALB/599/1979(H4N2),1979/08/11
    0 0 353 203 >A/mallard Dk/ALB/797/1983(H11N3),1983/08/23
    0 0 353 292 >A/Dk/Ohio/421/87(H7N8NS1),1987//
    0 0 353 529 >A/Ck/New Jersey/15906-9/96(H11N1),1996//
    0 0 353 1176 >A/American black Dk/North Carolina/675-075/04(H3N2),2004//
    0 0 353 1948 >A/green winged teal/CA/AKS1305/08(H11N9),2008/01/26

    mixin:
    So, because it's a bird disease, it needs to stay close to the origin to insure survival? But it's more free to mutate in humans?

    gs:
    I think it's because the index is optimized for waterborne transmission

    mixin:
    What is this 4th number: 15>A/turkey,,,

    gs:
    enumeration in my list



    gs:
    Sharon could post something there
    like : let's merge the forums
    or such

    mixin:
    you could suggest it in the Hopes and Dreams thread

    -----------------------------------------------


    The paper gives the results of sequence analysis of 150 positive samples in real-time RT-PCR, including 47 autopsy materials from patients (including 10 pregnant women), who died from fatal pneumonia mainly in November-December 2009, in whom the lifetime etiological diagnosis had not been made and h …

    70% of the primary materials from the deceased patients were found to have pandemic influenza A(H1N1) v mutants in the lung tissue with D222G (15%), D222N (15%), D222E (2%) substitutions, as well as a mixture of mutants (38%).

    Further spread of the mutants that have acquired a high virulence and preserved their capacity for the respiratory route of human infection may lead to the situation similar to that seen in the 1918-1919 pandemic

    ------------------------------------------------------------------


    four new (1-4) and 10 known (5-14) C-methylated flavonoids were isolated from a methanol extract of Cleistocalyx operculatus buds using an influenza H1N1 neuraminidase inhibition assay. Compounds 4, 7, 8, and 14, with a chalcone skeleton, showed significant inhibitory effects on the viral neuraminidases from two influenza viral strains, H1N1 and H9N2

    ------------------------------------------------------------------------


    Secondary attack rate for ****** = 45% in Quebec City
    The mean serial interval between onset of primary and confirmed secondary
    cases was 3.9 days
    ILI developed in 29% of household contacts

    Household transmission of pH1N1 may be substantially greater than previously estimated, especially in association with clinical presentations that include gastrointestinal complaints. Approximately 10% of pH1N1 infections acquired in the household may be asymptomatic.



    ---------------------------------------------------------------------------


    Of the 36,636 subjects recruited, 7292 received both PPV and TIV, 2076 received TIV vaccine alone, 1875 received PPV alone, and 25,393 were unvaccinated


    dual‐vaccinees experienced
    fewer deaths (hazard ratio [HR], 0.65;
    fewer cases of pneumonia (HR, 0.57;
    fewer ischemic strokes (HR, 0.67
    fewer acute myocardial infarction (HR, 0.52;
    fewer coronary admissions (HR, 0.59
    fewer intensive care admissions (HR, 0.45

    Dual vaccination with PPV and TIV is effective in protecting elderly persons with chronic illness from developing complications from respiratory, cardiovascular, and cerebrovascular diseases, thereby reducing hospitalization, coronary or intensive care admissions, and death.


    -----------------------------------------------------------------------------------


    n=773 vaxed against newflu
    ~6 weeks after vaccination, 12 developed the disease

    Two weeks after infection, vaccinated and unvaccinated patients had similar neutralizing antibody levels as the vaccinated control subjects

    Thereafter, the neutralizing antibody levels decreased markedly in vaccinated patients.

    Although the clinical consequences of infection are comparable between vaccinated and unvaccinated patients, humoral and cellular immune responses in vaccinated patients are boosted for some weeks, indicating an additional benefit of vaccination against 2009 pandemic influenza A (H1N1) virus.



    ---------------------------------------------------------------------------------

    On episode #101 of the podcast This Week in Virology, Vincent, Alan, Rich,
    Dickson,
    and Hamish review the three-dimensional structure of adenovirus, and the
    role of
    adenovirus type 36 in obesity.

    _http://www.virology.ws/_ (http://www.virology.ws/)


    --------------------------------------------------------------
    The salvage of human life ought to be placed above barter and exchange ~ Louis Harris, 1918

    Comment


    • #3
      Re: snippets

      cancer-thread, see also here:


      air-filters for cars, are they good for filtering viruses, air-cleaners ?
      ventilators,


      222G: http://www.ncbi.nlm.nih.gov/pubmed/20946414

      The efficacy of oral oseltamivir against symptomatic influenza was 76% (at 75 mg daily),
      and 73% (at 150 mg daily).
      Inhaled zanamivir 10 mg daily performed similarly.
      Neither NI had a significant effect on asymptomatic influenza.
      Oseltamivir induced nausea (odds ratio (OR) 1.79, 95% CI 1.10 to 2.93).
      Oseltamivir for post-exposure prophylaxis had an efficacy of 58% and 84% in two trials for households. Zanamivir performed similarly.


      It is a fact that in Viet Nam, Muscovy ducks are raised in large populations (approximately 8 million), usually kept in small flocks together with mallards and chickens. As a result, it is a great concern for epidemiologists to elucidate possible differences in relation to these species being expose …

      80% lethal in muscovi-ducks
      but 0-100% in mallards depending on the strain

      Our findings suggest a possible role of aerosol transmission of influenza in an acute ward setting. Source and engineering controls, such as avoiding aerosol generation and improving ventilation design, may warrant consideration to prevent nosocomial outbreaks.
      Our findings suggest a possible role of aerosol transmission of influenza in an acute ward setting. Source and engineering controls, such as avoiding aerosol generation and improving ventilation design, may warrant consideration to prevent nosocomial outbreaks.


      Viral hemagglutinin is involved in promoting the internalisation of Staphylococcus aureus into human pneumocytes during influenza A H1N1 virus infection.

      Conclusion: PHFGS can prevent influenza Pihui Fanggan Sachet

      Glycan Shielding of the Influenza Virus Hemagglutinin Contributes to Immunopathology in Mice.

      Qingdao were analyzed. Among the 3492 viruses, 101 carried a serine to proline substitution at position 128 (S128P) in the viral HA gene
      the prevalence of the S128P mutant was 33.56% (99/295) in a region of Eurasia including Russia, Mongolia, mainland China and South Korea, but only 0.11% (2/1846) in the rest of the world

      Erythrina addisoniae.

      Influenza A virus strains that circulate in humans differ in the ability of their NS1 proteins to block the activation of IRF3 and interferon-β transcription.

      Evidence of intercontinental transfer of North American lineage avian influenza virus into Korea.

      Sporadic human cases of swine-origin influenza before 2009 share the Sa epitope.

      Glycine 184 in the non-structural protein NS1 determines virulence of influenza A

      Qinghai-lake: Most of the high-risk species were from the family Anatidae,
      order Anseriformes (9/14 in spring, 11/15 in fall).
      Anatidae accounted for over 39% and over 91% of the total risk at spring and fall migration periods, respectively

      pubmed articles are badly formulated, not informative, IMO
      you have to read much to get some insight/understanding
      no good summaries and headlines

      ---------------------------------------

      there were papers early this year and I remember a formula (Kohn,Shaman
      transmission(temperature,absolute humidity)
      and I remember the charts with the Palese guinea pigs
      cold and dry and low UV --> tranmits,survives well


      > Don't confuse me.. I think Namru had an insertion, not a deletion.
      maybe, I'll have to check
      > but it's probably the same for either one?
      what was earlier ?
      > So if I look at the nucleotides and there is either an extra codon or a missing one,
      > then it's not an error.
      probably
      > For H5N1, is it just the amino acid position x 3 to find the nucleotides?
      in our enumeration : yes
      > When I get a nucleotide number like 355, is there an easy to tell if it's
      > in position 1,2 or 3 in the codon?
      you have to align first - or use mutatm, then see the rest when divided by 3


      The number of colds in children increases before the arrival of a flu. According to a study published in the Inserm Weekly Epidemiological Bulletin (BEH), there would be "a period of about one week between the early outbreaks of nasopharyngitis and the ILI.
      During three winter seasons, from 2005 to 2008, "we observed synchronization between elevated impact nasopharyngitis in children and the arrival of an episode of epidemic influenza," the researchers said. On three consecutive seasons, "the rise of incidence of nasopharyngitis preceded the arrival of the epidemic of influenza with a period of between 4 and 10 days," they said. Children could play a role of "triggers epidemics", the scientists suggest. Moreover, part of nasopharyngitis diagnosed in children may actually be cases of flu.
      The researchers determined that the data collected by the observatory Hivern @ the-KhiObs, whose objective is to disseminate information on these conditions, would contribute to already existing detection systems, such as that the Sentinel network on influenza. In sum, children with colds, flu nearby!

      ----------------------------------------

      death-statistics


      ----------------------------------------


      -------------------------
      15 suspected GBS cases after vaccination
      with 6M doses of Pandemrix --> 2.5 cases of GBS per M vaxed

      USA: ~60M infected and ~12000 deaths --> ~200 deaths per M infected

      Europe: 32M vaxed(Pandemrix), 38 cases of GBS --> 1.2 cases of GBS per M vaxed


      -----------------------------------

      ----------------------------------
      PB2 Residue 158 is a Pathogenic Determinant of Pandemic-H1N1 and H5 Influenza A Viruses in Mice.
      PB2 residue 271 plays a key role in enhanced polymerase activity in mammalian host cells

      PB2-E158G , travel1 mxpb2
      A/Auckland/1/2009

      more preferentially used codons of 09H1N1 are A-ended or U-ended

      Systemic influenza virus H5N1 infection in cats after gastrointestinal exposure.

      Conclusions  Chickens, turkeys and Pekin ducks are not susceptible to infection with
      dogflu by simulated respiratory exposure

      Equine influenza - surveillance and control.

      Historical thoughts on influenza viral ecosystems, or behold a pale horse, dead dogs, failing fowl,
      and sick swine. Morens DM, Taubenberger JK.

      The gastrointestinal manifestations with swine influenza (H1N1) in hospitalized adults.

      Gene expression signature-based screening identifies new broadly effective influenza a antivirals.

      The overall age-standardized proportion of people with seroprotective antibody levels was 46%
      British Columbia, U-shaped

      infecting sparrows and starlings with H3N8 --> little shedding

      Emergence of amantadine-resistant avian influenza H5N1 virus in India.

      98.Low Pathogenic Avian Influenza (H7N1) Transmission Between Wild Ducks and Domestic Ducks.

      A computational framework for influenza antigenic cartography.

      ------------------------------------------------
      I'm interested in expert panflu damage estimates
      my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

      Comment


      • #4
        Re: snippets

        in British adults each 10 nmol/l increase in 25(OH)D {vitamin D} was associated
        with a 7 % lower risk of infection
        -------------------------------------------------------------------
        The highest quality cluster-RCTs suggest respiratory virus spread can be prevented by hygienic measures, such as handwashing, especially around younger children. Benefit from reduced transmission from children to household members is broadly supported also in other study designs where the potential for confounding is greater. Nine case-control studies suggested implementing transmission barriers, isolation and hygienic measures are effective at containing respiratory virus epidemics. Surgical masks or N95 respirators were the most consistent and comprehensive supportive measures. N95 respirators were non-inferior to simple surgical masks but more expensive, uncomfortable and irritating to skin. Adding virucidals or antiseptics to normal handwashing to decrease respiratory disease transmission remains uncertain. Global measures, such as screening at entry ports, led to a non-significant marginal delay in spread. There was limited evidence that social distancing was effective, especially if related to the risk of exposure.
        ----------------------------------------------------------------------------
        Amino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contributes to mammalian pathogenesis.
        ----------------------------------------------------------------------------
        Results suggested that infection risk can be reduced by not recirculating air {in cars}; however,
        estimated risk ranged from 59% to 99·9% for a 90-min trip when air was recirculated in the newer vehicle
        -----------------------------------------------------------------------------
        Isolation and phylogenetic analysis of pandemic H1N1/09 influenza virus from swine in Jiangsu province of China.
        ---------------------------------------------------------------------
        We found higher mean virus titer and longer median infectious period for LPAI-infected ducks (10-11.5 days in oral and cloacal swabs) than HPAI-infected ducks (5 days) and geese (7.5 days)
        ---------------------------------------------------------------------
        Portuguese National Mortality Database of the Instituto Nacional de Estatística (National Statistics Institute in Portugal).
        Influenza epidemics have a substantial impact on human health, by increasing the mortality from pneumonia and influenza, respiratory and circulatory diseases, and all causes. This paper provides estimates of excess mortality rates associated with ...

        -------------------------------------------------------------------------
        Disease scenarios with case fatality rates (mild, moderate and severe) as 0.06%, 0.18% and 0.35%.
        clinical attack rate of 35%
        reduce UK-GDP by 0.3% (£3.5bn), 0.4% (£5bn) and 0.6% (£7.4bn)
        school closure 1.1% (£14.7bn), 1.3% (£16.3bn) and 1.4% (£18.5bn)
        most severe : 2.9% (£37.4bn), 3.2% (£41.4bn) and 3.7% (£47.5bn) loss to GDP
        There is concern regarding the impact that a global infectious disease pandemic might have, especially the economic impact in the current financial climate. However, preparedness planning concentrates more upon population health and maintaining a functioning health sector than on the wider economic …

        --------------------------------------------------------------------------------------------------
        In some cases, influenza and rhinovirus RNA can be detected on aircraft air filters, even ten days after the filters were removed from aircraft.
        ------------------------------------------------------------------------------------
        Efficacy of inactivating viruses by photocatalytically reacting nonwoven titanium dioxide fabric].
        --------------------------------------------------------------------------------------
        In China, domestic ducks and wild birds often share the same water, in which influenza viruses replicate preferentially. Isolation of 2 novel reassortant highly pathogenic avian influenza (H5N5) viruses from apparently healthy domestic ducks highlights the role of these ducks as reassortment vessels. Such new subtypes of influenza viruses may pose a pandemic threat.
        In China, domestic ducks and wild birds often share the same water, in which influenza viruses replicate preferentially. Isolation of 2 novel reassortant highly pathogenic avian influenza (H5N5) viruses from apparently healthy domestic ducks highlights the role of these ducks as reassortment vessels …

        -------------------------------------------------------------------------------------
        A total of 24,712 swab samples were collected from migratory and resident wild birds. Reverse transcription PCR showed a 0.7% HPAI (H5N1) prevalence. The highest prevalence was observed during January-February 2004 and March-June 2004, predominantly in central Thailand
        Transmission efficiency among poultry flocks was 1.7 X higher in regions with infected wild birds in the given or preceding month. The joint presence of wild birds and poultry is associated with increased spread among poultry flocks.
        ----------------------------------------------------------------------------------
        We found that a mutation at amino acid position 90 in the H5N1 HA, outside the receptor-binding domain (RBD), could simultaneously induce changes in the RBD conformation to escape from nAb binding and alter the receptor preference through long-range regulation. This mutation was deemed a "key event" for interspecies transmission. It is likely a result of positive selection caused by antibodies, allowing the original invasion by new species-specific variants. A mutation at amino acid position 160 in the RBD only induced a change in receptor preference. This mutation was deemed a "maintaining adaptation",
        ---------------------------------------------------------------------------------------
        Among the patients with H1N1 virus infection, 82.1% (55/67) patients presented with fever symptom, while more patients with high fever (≥ 39&#176;C) were found in seasonal influenza patients (P < 0.05). For the H1N1 patients, the median interval between the symptom onset and the undetectable RNA was six days (4 - 10 days). But viral shedding was still found in 31.3% patients after 7 days following illness onset. The median interval between disappearance of fever and an undetectable viral RNA level was three days (2 - 8 days), and 17.9% patients were found to be viral shedding 6 days later after normalization of body temperature. For the seasonal influenza patients, 94.6% patients were detected out viral RNA within 7 days. The median interval of seasonal influenza between the symptom onset and the undetectable RNA was four days (3 - 8 days). The median interval between disappearance of fever and an undetectable viral RNA level was three days (2 - 6 days).
        -------------------------------------------------------------------------------------
        A series of novel dihydro-alkyloxy-benzyl-oxopyrimidine derivatives were synthesized and evaluated for their activity against influenza virus in Madin-Darby canine kidney cells. Four dihydro-alkyloxy-benzyl-oxopyrimidine derivatives (4a1, 4a2, 4a3, and 4d1) showed potent activity against influenza v …

        dihydro-alkyloxy-benzyl-oxopyrimidine (DABO)
        4a3 was the most promising lead with broad activity against influenza
        ---------------------------------------------------------
        I'm interested in expert panflu damage estimates
        my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

        Comment


        • #5
          Re: snippets

          to make it clickable

          62858

          Evidence for limited exchange of avian influenza viruses between seaducks and dabbling ducks at Alaska Peninsula coastal lagoons.
          GM-CSF and Influenza: Will Saving Mice Help Humans?
          Excess Winter Mortality in France: Influence of Temperature, Influenza Like Illness, and Residential Care Status.
          Evolutionary dynamics of influenza A nucleoprotein (NP) lineages revealed by large-scale sequence analyses.
          Defective interfering influenza virus confers only short-lived protection against influenza virus disease: Evidence for a role for adaptive immunity in DI virus-mediated protection in vivo.
          Effectiveness of Seasonal Influenza Vaccine against Pandemic (H1N1) 2009 Virus, Australia, 2010.
          Evolution of influenza viral neuraminidase (NA) revealed by large-scale sequence analysis.
          Transmissibility of pandemic H1N1 and genetically related swine influenza viruses in ferrets.
          Influenza virus surveillance in migratory ducks and sentinel ducks at Poyang Lake, China.
          --------------------------------------------------------------------
          62875

          Netherlands May 2009 - May 2010 19/1100 had H275Y
          Enhanced surveillance of infections due to the pandemic A(H1N1) influenza virus, which included monitoring for antiviral resistance, was carried out in the Netherlands from late April 2009 through late May 2010. More than 1100 instances of infection with the pandemic A(H1N1) influenza virus from 200 …


          household quarantine was the most effective control measure, while school closure
          and household quarantine implemented together achieved the greatest benefit.
          Simulated results showed that household quarantine was the most effective control measure, while school closure and household quarantine implemented together achieved the greatest benefit. Agent-based models should be applied in the future to evaluate the efficacy of control measures for a range of …


          --------------------------------------------
          Amplitude of the seasonal change in day length increases with distance from the equator, and changes in day length markedly alter immune function in diverse nonhuman animal models of infection. Historical records of mortality data, ambient temperature, population density, geography, and economic ind …

          the longer the days (-->equator) the higher the mortality in 1918f

          62881
          -------------------------------------
          62912
          Quantity, not quality, of antibody response decreased in the elderly.
          Limited efficacy of inactivated influenza vaccine in elderly individuals is associated with decreased production of vaccine-specific antibodies.
          combinations of baicalein and ribavirin provide better protection against influenza infection than each compound used alone
          In severe pdmH1N1, viremia was common and the D222G/N mutant was found in half of the viremic patients.
          Triazavirin was efficient in protection of the animals from death. Its protective therapeutic efficacy (36.7+/-1.7&#37 was close to that of Oseltamivir (50.0+/-0.0%), comparable with that of Remantadin (38.3+/-1.7%) and higher than that of Arbidol (11.7+/-1.7%).
          H1N1-related hospitalization was significantly higher among American Indians (risk ratio [RR]=2.6; 95% confidence interval [CI]=2.2, 3.2), Blacks (RR=1.7; 95% CI=1.2, 2.4), and Hispanics (RR=1.8; 95% CI=1.5, 2.0) than it was among non-Hispanic Whites
          -----------------------------------------------------------------------------
          63022
          Reassortment Events Among Swine Influenza A Viruses in China: Implications for the Origin of the 2009 Influenza Pandemic.
          Despite frequent reassortment events between TR and EA lineage viruses in China, evidence for the genesis of the 2009 pandemic virus in pigs in this region is still absent.
          waterfowl captured in Minnesota and South Dakota. We screened a total of 7260 cloacal samples from waterfowl for MP and H5 PCR and found 148 H5.Inoculating 71 of these embryonated chicken eggs,
          25 gave H5 AIV.




          Vaccine Effectiveness in Germany 2009 :
          <14years: 79% and 87%
          >14years: 70% and 74%

          We found that the clinical attack rate in 1889 was relatively high and constant between the ages of 1 and 60 years, but was lower outside of the extremes of this age range. By contrast, age-specific influenza-related mortality rates were J-shaped and increased with age beyond 20 years. We conclude that the age-specific attack rates of the 1889 pandemic were most similar to those of the 1968 pandemic and that influenza-related mortality rates did not follow a W-shaped curve as was observed during the 1918 pandemic.

          [in Britain] Russian influenza had taken the form of three, and possibly four, distinct waves of infection,
          with the second wave in the spring of 1891 proving more lethal than the first wave, and the third wave
          in the winter of 1892 proving almost as lethal again.

          -------------------------------------------------------------------------------
          I'm interested in expert panflu damage estimates
          my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

          Comment


          • #6
            Re: snippets

            a large Open Reading Frame (ORF), segment 8 , humans.
            [29] [30].
            167 aa
            144-293
            NEG8 ORF is shown as a solid red arrow; the 168-216 region of the 216 codon NEG8 ORF is shown as a dashed red arrow.

            793-293 backwards = 167*3
            293-144 backwards =

            529-864=NEP
            27-738=NS1
            Influenza A virus genomes are comprised of 8 negative strand single-stranded RNA segments and are thought to encode 11 proteins, which are all translated from mRNAs complementary to the genomic strands. Although human, swine and avian influenza A ...


            TAG stop codon of the NEG8 ORF into TAT,

            216 codon NEG8 ORF (Figure ​(Figure3).3). Of 1739 true human after 1950, 1677 have >= 216
            I'm interested in expert panflu damage estimates
            my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

            Comment


            • #7
              Re: snippets

              63135
              A/Wenshan/01/2009 H1N1 induces apoptotic cell death in human airway epithelial cells.
              RGLFGAIAGFIE(HA,344), YNAELLV(HA,438),AIAGFIE(HA,349) ,
              RTQSEC(NA,?),EECSYP(NA,?) which on reveres translation
              provided siRNA sequences as potential therapeutic candidates

              Patchouli Alcohol
              lower antibody response to Mx if previous seasonal vax.(Korea) , original antigenic sin
              N385(6),V407(6),D225E(4),A189T(4),P300S(4) in 1/10 HIV patients
              risk for H5N1 in Indo:elevation", "human population density" and "rice cropping" ,"human settlements" and "road length", but not: domestic backyard ducks
              1169 PCR confirmed flu-hospitalizations in Oz,2010: 24&#37; of H1N1/09 cases, 43% of seasonal
              and 54% of controls were vaxed
              allele A NS1 proteins of H6N8 and H4N6 are significantly better in preventing dsRNA induced
              NF-kappaB promoter activation compared to allele B
              It is now clear that wild birds may be responsible for primary introduction in a previously free area. To date it is still unclear whether HPAI infection may be maintained in wild bird populations for extended periods of time
              An equine herpesvirus 1 (EHV-1) vectored H1 vaccine protects against challenge with swine-origin influenza virus H1N1.
              we observed sequence variations between sequential samples from the same person and also within the same household
              Aprotinin and similar protease inhibitors as drugs against influenza.
              seropositiv Mex.swine workers: unexposed and exposed
              28%,19% to A/NewCaledonia/20/99(1N1)
              68%,34% to A/Panama/2001/99(H3N2)
              2% and 13% to A/Sw/England/163266/87(H3N2)
              0% and 3% to A/Sw/Wisconsin/238/97(H1N1)
              ---------------------------------------
              I'm interested in expert panflu damage estimates
              my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

              Comment


              • #8
                Re: snippets

                hello gsgs :
                thank you. this one is very good.It seems like " kawaoka and coll " paper :-)

                Comment


                • #9
                  Re: snippets

                  apparantly the sequence A/Wenshan/01/2009 is not available.
                  Is(was) it widespread ?
                  several others from Shandong-Wenshan are availabe
                  ------------------------------------------------------------#
                  63204
                  The household transmissions vary almost linearly with the household size
                  4&#37; of transmissions would occur on the subway(NY)
                  -----------------------------
                  Characterization of Influenza A Outbreaks in Minnesota Swine Herds and Measures Taken to Reduce the Risk of Zoonotic Transmission.
                  -----------------------------
                  Influenza A Pandemic (H1N1) 2009 Virus Outbreak in a Cat Colony in Italy.
                  ==================================================--
                  there are characteristics of communities that drive influenza transmission dynamics apart from individual and household level risk factors, and that such factors have effects independent of strain
                  --------------------------------------------
                  different lineages obtained through a 13-y surveillance program in southern China
                  respiratory-droplet transmissibility (albeit inefficient) in ferrets only in the TRsw- A/Sw/HK/915/04
                  {also in Mx ? use this to identify possible new pandemic candidates}
                  -----------------------------------------------
                  63223
                  1-5 cups/day of green tea may prevent influenza infection in children
                  ---------------------------------------------------
                  63390
                  Anti-influenza virus activity of tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone.
                  ultraviolet germicidal irradiation (UVGI), microwave-generated steam, and moist heat
                  were all effective to decontaminate N95 masks (3M models 1860s and 1870)
                  Antiviral activity of the MEK-inhibitor U0126 against pandemic H1N1v and highly pathogenic avian influenza virus in vitro and in vivo.
                  Differential Contribution of PB1-F2 to the Virulence of Highly Pathogenic H5N1 Influenza A Virus in Mammalian and Avian Species.
                  Outbreak of swine influenza in Argentina reveals a noncontemporary human H3N2 virus highly transmissible among pigs.
                  Reversion of PB2 627E to 627K during replication of an H5N1 clade 2.2 virus in mammalian hosts depends on origin of the nucleoprotein.
                  Novel reassortment of Eurasian Avian-like and pandemic/2009 influenza viruses in swine: infectious potential to humans.
                  Altered Pathogenicity for Seasonal Influenza Virus by Single Reassortment of the RNP Genes Derived From the 2009 Pandemic Influenza Virus.
                  --------------------------------------------------------------
                  I'm interested in expert panflu damage estimates
                  my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                  Comment


                  • #10
                    Re: snippets

                    In contrast to other mammalian hosts (mice, ferrets, and macaques) where infection with the 1918/rec virus was lethal, the pigs did not develop severe respiratory distress or become moribund
                    the 1918 pandemic influenza virus was able to infect and replicate in swine, causing a respiratory disease, and that the virus was likely introduced into the pig population during the 1918 pandemic, resulting in the current lineage of the classical H1N1 swine influenza viruses.
                    Swine influenza was first recognized as a disease entity during the 1918 "Spanish flu" pandemic. The aim of this work was to determine the virulence of a plasmid-derived human 1918 pandemic H1N1 influenza virus (reconstructed 1918, or 1918/rec, virus) in swine using a plasmid-derived A/swine/Iowa/15 …


                    -------------------------------------------------------------------

                    Influenza A viruses are endemic in many animal species, including humans, swine, and wild birds, and sporadic cases of transmission of influenza A viruses between humans and animals do occur, including human infections with avian-origin influenza A viruses (i.e., H5N1 and H7N7) and swine-origin infl …

                    Swine-Origin (H3N2) Infection in 2 Children --- Indiana and Pennsylvania, July--August 2011.

                    A remarkable feature of the 2009 pandemic H1N1 influenza virus is its efficient transmissibility in humans compared to that of precursor strains from the triple-reassortant swine influenza virus lineage, which cause only sporadic infections in humans. The viral components essential for this phenotyp …

                    The M segment of the 2009 new pandemic H1N1 influenza virus is critical for its high transmission efficiency in the guinea pig model.

                    Cross-protective immunity to influenza pH1N1 2009 viruses induced by seasonal A(H3N2)
                    virus is mediated by virus-specific T cells.

                    Virulence and genetic compatibility of polymerase reassortant viruses derived from the pandemic (H1N1) 2009 influenza virus and circulating influenza A viruses.
                    reassortment between the pandemic A/California/04/09 (CA04, H1N1) and current human and animal influenza viruses produced variants possessing a broad spectrum of pathogenicity in the mouse model.
                    Although most polymerase reassortants had attenuated pathogenicity (including those containing seasonal human H3N2 and high-pathogenicity H5N1 virus segments) compared to that of the parental CA04 (H1N1) virus, some recombinants had significantly enhanced virulence. Unexpectedly, one of the five highly virulent reassortants contained a A/Swine/Korea/JNS06/04(H3N2)-like PB2 gene with no known virulence factors

                    Modifications in the polymerase genes of a swine-like triple-reassortant influenza virus to generate live attenuated vaccines against 2009 pandemic H1N1 viruses.
                    modified A/turkey/OH/313053/04 (H3N2) (ty/04), (LAIV). this vaccine is highly attenuated in mice
                    A single immunization with the ty/04 att-based vaccines conferred complete protection against a lethal H1N1pdm virus infection in mice

                    Immunity to pre-1950 H1N1 influenza viruses confers cross-protection against the pandemic swine-origin 2009 A (H1N1) influenza virus.

                    Alternative live-attenuated influenza vaccines based on modifications in the polymerase genes protect against epidemic and pandemic flu.

                    Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice.


                    -----------------------------------------------------------------
                    I'm interested in expert panflu damage estimates
                    my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                    Comment


                    • #11
                      Re: snippets

                      63764
                      Characterisation of a highly pathogenic H5N1 clade 2.3.2 influenza virus isolated from swans in Shanghai, China.
                      -------------------------------------
                      Avian influenza. Warning of H5N1 resurgence surprises community.
                      --------------------------------------
                      Temporal dynamics of host molecular responses differentiate symptomatic and asymptomatic influenza a infection.
                      ------------------------------------------
                      A molecular and antigenic survey of H5N1 highly pathogenic avian influenza virus isolates from smallholder duck farms in Central Java, Indonesia during 2007-2008.
                      --------------------------------------------
                      Multiple Reassortment between Pandemic (H1N1) 2009 and Endemic Influenza Viruses in Pigs, United States.
                      ------------------------------------------------
                      During an epidemiologic investigation of pandemic influenza (H1N1) 2009 virus infection in May 2009 in Osaka, Japan, we found 3 clusters in which virus transmission occurred during the presymptomatic phase.
                      -----------------------------------------------------
                      Influenza A facilitates sensitization to house dust mite in infant mice leading to an asthma phenotype in adulthood.
                      ------------------------------------------------
                      The M segment of the 2009 new pandemic H1N1 influenza virus is critical for its high transmission efficiency in the guinea pig model.
                      ------------------------------------------------
                      Specifically, we suggest that infection with a novel virus strain, in people previously exposed to influenza viruses, can elicit a memory B cell response against conserved hemagglutinin stalk epitopes and/or neuraminidase epitopes. The anti-stalk and/or anti-neuraminidase antibodies then act to diminish the clinical severity of disease caused by novel influenza viruses and to eliminate seasonal virus strains.
                      Novel pandemic influenza viruses enter the human population with some regularity and can cause disease that is severe and widespread. The emergence of novel viruses, historically, has often been coupled with the disappearance of existing seasonal ...

                      .-------------------------------------------
                      63631
                      ---------------------------------------
                      By examining in vitro polymerase activities and in vivo pathogenicities in mice model, we were able to show that individual or simultaneous expression of virulence factors in PB2, PB1, and PA might not significantly elevate pathogenicity. Nevertheless, we demonstrated that PB2 627K or PA 97I derived from different genetic backgrounds and other unknown polymerase markers have the potential to enhance virulence of CA04. Virus rescue and replication studies identified PA as a critical factor in maintaining genetic stability of the CA04 (H1N1) virus.
                      --------------------------------------------------------
                      Distribution of sialic acid receptors and influenza A virus of avian and swine origin in experimentally infected pigs.
                      --------------------------------------------------------
                      Using several in vitro models of virus replication, we observed increased replication for a reassortant CA/09 virus expressing the hemagglutinin (HA) gene of HK/483 (CA/09-483HA) relative to either parental CA/09 virus or reassortant CA/09 expressing other HK/483 genes. This increased replication correlated with enhanced pathogenicity in infected mice similar to the parental HK/483 strain. Serial passage of the CA/09 parental virus and the CA/09-483HA virus through primary human lung epithelial cells resulted in increased pathogenicity, suggesting that these viruses may easily adapt to humans and become more virulent. In contrast, serial passage attenuated the parental HK/483 virus in vitro, and resulted in slightly reduced morbidity in vivo, suggesting that sustained replication in humans may attenuate H5N1 avian influenza viruses. Together, these data suggest that reassortment between co-circulating human pH1N1 and avian H5N1 influenza strains may result in a virus with the potential for increased pathogenicity in mammals.
                      A novel H1N1 influenza virus emerged in 2009 (pH1N1) to become the first influenza pandemic of the 21st century. This virus is now cocirculating with highly pathogenic H5N1 avian influenza viruses in many parts of the world, raising concerns that a reassortment event may lead to highly pathogenic in …

                      ---------------------------------------------------------------
                      Towards universal influenza vaccines?
                      .................................................. ..............
                      Multiple Reassortment between Pandemic (H1N1) 2009 and Endemic Influenza Viruses in Pigs, United States.
                      ---------------------------------------------------------
                      Weather conditions coinciding with peak human H5N1 incidence in Egypt suggest that human infection may be occurring primarily via droplet transmission from close contact with infected poultry
                      -----------------------------------------------------------
                      there may have been an early epidemic in Mexico City as well as in all of Mexico during February/March.
                      t the early epidemic in Mexico City in February/March had been more transmissible (larger R(0)) and peaked earlier than the rest of the country
                      could have already spread to other continents prior to the identification
                      ------------------------------------------------------------
                      significant variations exist, even between closely related avian species,
                      ------------------------------------------------------------
                      I'm interested in expert panflu damage estimates
                      my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                      Comment


                      • #12
                        Re: snippets

                        In 2009, a novel H1N1 influenza (pH1N1) virus caused the first influenza pandemic in 40 y. The virus was identified as a triple reassortant between avian, swine, and human influenza viruses, highlighting the importance of reassortment in the generation of viruses with pandemic potential. Previously, …

                        H9N2 avian influenza viruses and pH1N1 viruses, both of which have occasionally infected pigs, have the potential to reassort and generate novel viruses with respiratory transmission potential in mammals.
                        ------------------------------------------------------------
                        Surveillance of swine influenza viruses (SIVs) in 31 pig farms in northern and southern parts of Vietnam was conducted. Six H3N2 influenza A viruses were isolated from a pig farm in southern Vietnam. They were novel genetic reassortants between a triple-reassortant SIV and a human seasonal H3N2 viru …

                        6 reassortants in swine on a Vietnam swine farm: HA and NA from human H3N2 (2004-2006)
                        rest from triple-swine
                        --------------------------------------------------------------
                        Reverse genetic analysis showed that a mutation at residue 82, 141, or 189 of the HA protein
                        promotes virus replication in the presence of the NA H274Y mutation. (old seasonal H1N1)
                        ----------------------------------------------------------------
                        (N125D and N156K) or (N125D and E374K) --> vaccine escape (******)
                        --------------------------------------------------------------
                        13 1918 autopsy cases G222 (summer) --> D222 (fall)
                        no genetic difference summer,fall
                        -------------------------------------------------
                        The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m …

                        Adenovirus vax afgainst flu
                        ------------------------------

                        not enough capacity ? but it can be increased ? that's what
                        they told us in 2006, why we need cell-based.
                        -------------------------------
                        I'm interested in expert panflu damage estimates
                        my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                        Comment


                        • #13
                          Re: snippets



                          Shedding in highly immunocompromised persons may last weeks to months.
                          Compared with adults, children can shed virus earlier before illness begins and for longer
                          periods once illness starts
                          In 1 report, at least 4 illnesses (8&#37; of the total) in children were associated with
                          presymptomatic shedding that began6, 4, 3, and 3 days, respectively, before illness onset.[5]

                          The median duration of virus detection is typically 7−8 days after illness onset, but shedding
                          for up to 21 days has been recorded.

                          In 1 study, virus was shed by 10% of children on days 8−11, by 5% on days 12−15,
                          and by 0% on days 16−19.[6]

                          A better understanding of transmission dynamics is essential in influenza pandemic planning. If a substantial proportion of transmissions were to occur during the presymptomatic phase or from asymptomatic individuals, then infection control measures ...

                          The proportion of transmission by asymptomatic individuals, defined as Theta (Τ) in transmission
                          models, is assumed to be one-third to one-half that of influenza-infected symptomatic individuals.2,8,9

                          During an epidemiologic investigation of pandemic influenza (H1N1) 2009 virus infection in May 2009 in Osaka, Japan, we found 3 clusters in which virus transmission occurred during the presymptomatic phase. This finding has public health implications because it indicates that viral transmission in c …

                          During an epidemiologic investigation of pandemic influenza (H1N1) 2009 virus infection
                          in May 2009 in Osaka, Japan, we found 3 clusters in which virus transmission occurred
                          during the presymptomatic phase.

                          Possible healthcare-associated pH1N1 transmission was identified in 9 (14%) of 63 exposed HCP;
                          6 (67%) of 9 seropositive HCP had asymptomatic infection.

                          T-cell immune responses and asymptomatic H5N1 influenza infection.
                          Epstein S.

                          NY,May,2009:
                          79 household contacts in 28 households, 19% had serologically confirmed pH1N1 infection,
                          28% of those infected were asymptomatic. Serologically confirmed infection
                          by age of contacts: <10y:36%, 10-18y: 46%,19-54y: 8%, >55y:22%

                          very low probability of asymptomatic infection in 1918
                          high frequency of prior immunity due to the spring wave (or perhaps to cross-reactive immunity)

                          Apr2008-Aug2009, Thailand,991(16&#183;5%) of 5995 pediatric ILI patients tested positive.
                          442 index children with 1147 household members, 221<6y.
                          397(89&#183;8%)index patient slept in the parents' bedroom.
                          2ndary attack rate = 21&#183;5%, 56/345(16&#183;3%) 2ndary cases were asymptomatic.

                          Berlin:84 households with 82, 69 and 67 contacts,2009/10-2010/11
                          2ndary AR =16% (35/218). facemasks and hand hygiene effective

                          The lack of age-pattern in seropositivity is unexpected, perhaps highlighting the
                          importance of children as asymptomatic transmitters of influenza in households

                          Boarding school,pH1N1, n=1570,infection rate=32%,attack rate=22.2%,
                          asymptomatic infection rate=9.9% Sharing a classroom OR=2.17 dormitory space OR=2.32
                          Opening windows did help

                          9.4% asymptomatic in Quebec
                          ------------------------------------------
                          .in Portugal flu gave 247/M all-cause excess deaths
                          3-6 fold higher for A(H3N2) vs.H1N1/B.
                          1980–2004
                          all cause excess deaths = 2475 rate=26/100000 , 3% of total deaths Oct-May
                          cerebrovascular:3.2%
                          ischemic heart disease:2.0%
                          -----------------------------------------
                          Influenza virus infection triggers a cytokine cascade that aggravates ischemic brain
                          damage and increases the risk of intracerebral hemorrhage after tissue plasminogen activator treatment.
                          ----------------------------------------
                          To our knowledge, there are no reports of asymptomatic infections with influenza A/H1N1 in
                          immunosuppressed patients. We present the first case of a young heart transplant recipient
                          who remained asymptomatic despite positive polymerase chain reaction (PCR) after exposure
                          to individuals with influenza A/H1N1.
                          -----------------------------------------
                          Increased levels of acute phase proteins (APP) in serum are associated with vulnerability of atherosclerotic plaques and acute manifestations of coronary heart disease (CHD). APP have been viewed as indexes of active vascular inflammation or as mediators of atherothrombosis. In the present study we …

                          acute phase proteins (APP) plasma C-reactive protein , serum amyloid A (SAA)
                          ------------------------------------------------------------------------------
                          influenza A infection led to a dramatic increase in gene expression, particularly of TLRs,
                          C-type lectin receptors, several complement components, as well as FcεR1.
                          Vaccination induced a prominent APR with increased WBC, elevated blood
                          levels of SAA and fibrinogen, and decreased serum iron concentrations
                          -----------------------------------------

                          Antiviral effects of Psidium guajava Linn. (guava) tea http://www.ncbi.nlm.nih.gov/pubmed/22453134
                          Capture of influenza viruses and prevention of their infection by coral mineral powder (sango mineral powder).
                          The anti-influenza virus activity of fossilized marine coral powder (sango mineral powder, SMP) was studied. SMP is composed in terms of mass of around 25 % of calcium and 10 % of magnesium, respectively, principally as dolomite (CaMg(CO(3))(2)) but not as calcium oxide (CaO) or magnesium oxide (MgO …

                          Evaluation of the antiviral activity of a green tea solution as a hand-wash disinfectant.
                          Protective effect of ginseng polysaccharides on influenza viral infection.
                          Antiviral effect of the egg wax of Amblyomma cajennense (Acari: Ixodidae).
                          novel compound with antiviral activity depending on PA subunit of viral RNA polymerase.
                          -----------------------------------------------
                          Moderate influenza vaccine effectiveness in Victoria, Australia, 2011.
                          --------------------------------------------------
                          IFITM3 restricts the morbidity and mortality associated with influenza.
                          IFITM3 Inhibits Influenza A Virus Infection by Preventing Cytosolic Entry
                          To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein ...

                          --------------------------------------------------
                          Detection of influenza A virus based on fluorescence resonance energy transfer from quantum
                          dots to carbon nanotubes.
                          --------------------------------------------------------------
                          We have demonstrated, using Segminator II, that it is possible to distinguish platform specific
                          error from biological variation using data derived from two different platforms
                          ----------------------------------------------------------------
                          Influenza vaccination has a modest but significant effect on prevention of hospitalization for pneumonia and influenza in persons 50 years of age and older.

                          69000 hospitalizations,USA-CA.
                          flu-vax gave ~10% fewer hospitalizationd for P+I but no significant effect on hospitalizations
                          for ischemic heart disease (IHD), congestive heart failure (CHF), cerebrovascular disease (CVD), or trauma.

                          Dual vaccination with PPV and TIV is effective in protecting elderly persons with chronic illness from developing complications from respiratory, cardiovascular, and cerebrovascular diseases, thereby reducing hospitalization, coronary or intensive care admissions, and death.

                          n=36636,pneumovax+fluvax ->35% (or 65%?)fewer deaths from all causes, 43% from pneumonia,
                          33% ischemic stroke,48% from acute myocardial infarction. 41% fewer coronary admissions
                          55% fewer intensive care submissions
                          ------------------------------------------
                          The substantial winter influenza peak in temperate climates has lead to the hypothesis that cold and/or dry air is a causal factor in influenza variability. We examined the relationship between cold and/or dry air and daily influenza and pneumonia mortality in the cold season in the New York metropo …

                          association between periods of low dew point temperature and above normal pneumonia and
                          influenza mortality 17 days later in New York
                          ------------------------------------------
                          Influenza cases increased significantly with minimal temperatures and high visibility.
                          in Niamey, Niger , 2009/10

                          -----------------------------------
                          EPs&#174; 7630 (Umckaloabo&#174, an extract from Pelargonium sidoides roots,
                          exerts anti-influenza virus activity in vitro and in vivo.
                          ------------------------------
                          Our results do not support the hypothesis that high doses of vitamin D supplementation will have a pronounced effect on influenza-like disease in populations not targeted for high influenza risk.

                          Our results do not support the hypothesis that high doses of vitamin D supplementation
                          will have a pronounced effect on influenza-like disease in populations not targeted for
                          high influenza risk.
                          -----------------------------------------------------------------------------------------
                          unlikely that seasonal variations in vitamin D levels principally determine the seasonality
                          of influenza in temperate regions.
                          Seasonal variation in serum concentration of the vitamin D metabolite 25(OH) vitamin D [25(OH)D], which contributes to host immune function, has been hypothesized to be the underlying source of observed influenza seasonality in temperate regions. ...

                          -------------------------------------------------------------------------------------------
                          Declining serum concentrations of 25-hydroxyvitamin D seen in the fall and winter as distance increases from the equator may be a factor in the seasonal increased prevalence of influenza and other viral infections. This study was done to determine ...

                          Concentrations of 38 ng/ml or more were associated with a significant (p<0.0001)
                          two-fold reduction in the risk of developing acute respiratory tract infections
                          .................................................. ........................................

                          In temperate latitudes even pandemic influenzas often show a clear seasonality.
                          The data support the hypothesis that high fluences of UVB radiation (vitamin D level),
                          as occur in the summer, act in a protective manner with respect to influenza.
                          ----------------------------------------------

                          association between climate factors and the seasonality of influenza A was complicated
                          The effect of the factors including mean temperature, relative humidity, wind speed and
                          absolute humidity on the seasonality of influenza A were similar
                          ---------------------------------------------------------------------
                          Our results also indicate that, like in the case for influenza A virus, transmission of influenza B
                          viruses is enhanced at colder temperatures, providing an explanation for the seasonality of
                          influenza epidemics in temperate climates.
                          -----------------------------------------------------------------------
                          When a weather-dependent ILI factor is included in the model, the epidemic size of influenza-like
                          illness becomes dependent not only on the susceptibility of the population at the beginning of the
                          epidemic season but also on the weather conditions during which the epidemic unfolds.
                          ------------------------------------------------------------------------
                          With the occurrence of pandemic influenza A (H1N1) 2009, we observed a change in the seasonality
                          of influenza activity in Hong Kong with a seemingly corresponding change in the activity of respiratory
                          syncytial virus,parainfluenza virus, and adenovirus during 2009-2011. This phenomenon could most
                          likely be explained by virus interference.
                          -----------------------------------------------------------------------------------
                          Variations in mortality were also related to extreme temperatures: cold winters periods and hot
                          summers periods were associated with excess mortality in Denmark
                          ---------------------------------------------------------------------------------
                          Influenza in Thailand annually presents two peaks, a major peak during the rainy season
                          (June-August) and a minor peak in winter (October-February)
                          ---------------------------------------------------------------------------------
                          Peak influenza activity was observed from June to August in Delhi, Pune, and Kolkata
                          and October to December in Chennai. Maximum influenza activity was seen during the
                          rains in Delhi, Pune, Chennai, and Kolkata in correlation with virus isolations.
                          Year-round limited influenza activity with peaks during rains was observed
                          ---------------------------------------------------------------------------------------
                          Higher levels of PM(10) and NO(2), which are typically markers of traffic related pollution,
                          seem to be associated with transiently increased risk of myocardial infarction 1-6 hours
                          after exposure, but later reductions in risk suggest that air pollution may be associated
                          with bringing events forward in time ("short-term displacement") rather than increasing
                          overall risk. The well established effect of air pollution on cardiorespiratory mortality may
                          not be mediated through increasing the acute risk of myocardial infarction, but through
                          another mechanism.
                          -------------------------------------------------------------------------------------
                          absolute humidity stronger associated with flu in Japan than relative humidity
                          AH at flu onset in 7 and 12 in subsidience out of 14 seasons, respectively.
                          AH strongly correlates with the onset and subsidence of influenza epidemics.
                          ----------------------------------------------------------------------------------------
                          Shortcomings of vitamin D-based model simulations of seasonal influenza
                          ------------------------------------------------------------------------------------
                          The results indicated an association between coarser PM fractions (PM10 and PM10-2.5)
                          and respiratory-specific mortality on the one hand, and between PM2.5 and cardiovascular
                          -specific mortality on the other. While the risk of mortality due to exposure to particulate
                          matter was greater in summer than in winter, this difference was statistically significant
                          solely for total organic-cause mortality. (Madrid)
                          -------------------------------------------------------------
                          Influenza was highly seasonal in southern Africa.
                          ----------------------------------------------------------------
                          The central questions in influenza seasonality remain unresolved
                          Despite the significant disease burden of the influenza virus in humans, our understanding of the basis for its pronounced seasonality remains incomplete. Past observations that influenza epidemics occur in the winter across temperate climates, ...

                          Further, recent evidence points to a link between increased influenza activity and the rainy
                          season in several tropical populations
                          Shaman et al. (2010) showed that the relationship between AH and virus survival is
                          consistent with observations in the United States where anomalously low AH conditions
                          generally precede the onset of influenza epidemics by approximately 2 weeks.
                          The hypothesis that seasonal variation in immunity explains influenza seasonality is supported
                          by observations that humans are less likely to experience influenza-like symptoms after manual
                          inoculation with influenza viruses during interepidemic periods relative to common epidemic
                          months (Shadrin et al. 1977).
                          ----------------------------------------------------------------------------
                          Antiviral activity of crude extracts of Eugenia jambolana Lam. against H5N1
                          -----------------------------------------------------
                          66806 , 2012-04-09
                          With the occurrence of pandemic influenza A (H1N1) 2009, we observed a change in the seasonality
                          of influenza activity with a seemingly corresponding change in the activity of respiratory syncytial virus,
                          parainfluenza virus, and adenovirus during 2009-2011. This phenomenon could most likely be explained
                          by virus interference
                          ---------------------------------------------
                          The model presented provides a robust and reasonable approach to estimating the number of deaths caused by influenza and RSV by age group at the end of each winter.

                          In England and Wales 7000-25000 deaths were associated with influenza in the winter periods 1999-2009.
                          2.5-8.1% of deaths in >75y were caused by influenza.
                          RSV accounted for 5000-7500 deaths each winter season
                          -------------------------------------------------------------------------------------------------
                          in USA among adults aged >65 years ~ 14000 deaths a year have been attributed to RSV
                          ---------------------------------------------------------------------------------------------

                          -------------------------------------------
                          66837,2012.04.12
                          Quantification of dust-borne transmission of highly pathogenic avian influenza virus between chickens.
                          ---------------------------------------------------------------------------------
                          We have documented a substantial and ongoing increase in the number of ARI hospitalizations during the period December 2011-February 2012 and an older age distribution of laboratory-confirmed A/H1N1 influenza hospitalizations and deaths, relative to 2009 A/H1N1 pandemic patterns. The gradual change …

                          ----------------------------
                          66971
                          ----------------------------------------------------------
                          H5N1 in Cambodia
                          Persistence of infectious virus did not exceed 4 days, and was only identified in rain water.
                          No infectious virus particles were detected in pond and lake water or mud even when high
                          inoculum doses were used. However, viral RNA persisted up to 20 days in rain water
                          and 7 days in pond or lake water. Viral RNA was also detected in mud samples,
                          up to 14 days post-contamination in several cases. Infectious virus and viral RNA was detected
                          in few cases in the aquatic fauna and flora, especially in bivalves and labyrinth fish,
                          although these organisms seemed to be mostly passive carriers of the virus rather than
                          host allowing virus replication.
                          -------------------------------------------------------------------------------
                          Between 2003 and 2008, seasonal influenza, particularly that caused by the influenza B virus, was associated with substantial mortality in three cities in the temperate north of China and five cities in the subtropical south of the country.

                          ~180 yearly deaths per M due to flu in N-China cities 2003-2008 and ~113 in S-China cities.
                          124 resp. 88 of these from respiratory and circulatory
                          Mortality wa higher for B than for A.
                          [their chart for S.city deaths seems not to well correlate with flu - no impact of Aug.-waves
                          and deaths increase before flu starts]
                          -----------------------------------------------------------------------------------
                          346(14%) out of 2500 deaths in Bangladesh 2009 in selected hospitals had ILI before death
                          estimated flu-deathrate = 110/M/y , 125 for >59
                          -------------------------------------------------------------------------------------
                          Person-to-person transmission of influenza viruses occurs by contact (direct and fomites) and non-contact (droplet and small particle aerosol) routes, but the quantitative dynamics and relative contributions of these routes are incompletely ...

                          3 strains of pH1 transmitted well by aerosols in ferrets
                          1 strain of oH1 transmitted badly, the better aerosol x the less virulent
                          level of exhaled virus, infectious particle size, mucosal inflammation, viral replication efficiency
                          ------------------------------------------------------------------------------------------------
                          67002
                          D282 was active against influenza A and B viruses by inhibiting de novo pyrimidine biosynthesis. Although effective in vitro, the compound, like others in its class, was devoid of antiviral activity in infected mice.

                          A non-nucleoside, 4-[(4-butylphenyl)amino]-2-methylene-4-oxo-butanoic acid (D282),
                          inhibited influenza A and B CPE by 50% at 6-31 &#181;M.
                          Ribavirin at 14-44 &#181;M . D282 and ribavirin inhibited virus yield by 90%
                          at 9.5&#177;3.3 and 10.8&#177;3.2 &#181;M, respectively.
                          D282 (<100 mg/kg/day) failed to prevent death of mice infected with influenza.
                          ----------------------------------------
                          No Association between 2008-09 Influenza Vaccine and Influenza A(H1N1)pdm09
                          Virus Infection, Manitoba, Canada, 2009.
                          -----------------------------------------
                          To provide an early estimate of 2011/12 influenza vaccine effectiveness (VE), we conducted a multicentre case-control study based on seven sentinel surveillance networks. We included influenza-like illness cases up to week 7/2012 from the vaccination target groups, swabbed less than eight days after symptom onset. Laboratory-confirmed influenza A(H3) cases were compared to negative controls. Adjusted VE was 43% (95% confidence interval: -0.4 to 67.7), suggesting low to moderate VE against influenza A(H3) in the early 2011/12 season.

                          Adjusted VE was 43% in europe 2011/12
                          ---------------------------------------
                          67025
                          In vitro anti-influenza virus and anti-inflammatory activities of theaflavin derivatives.
                          --------------------------------
                          67121
                          enzymatic polymerisation confers immunoactivating potential to phenylpropanoic acids,
                          and CD4 plays a key role in their cytokine-inducing activity.
                          -----------------------------------------------------------------
                          67202
                          Here, we describe the difference in predicted RNA secondary structure stability that exists between avian, swine and human coding regions. The results predict that global ordered RNA structure exists in influenza A segments 1, 5, 7 and 8, and that ranges of free energies for secondary structure formation differ between host strains
                          -----------------------------------------
                          a Vero cell-based HPAI H5N1 vaccine candidate (H5N1/YNVa) with stable high yield was achieved by reassortment of the Vero-adapted (Va) high growth A/Yunnan/1/2005(H3N2) (YNVa) virus with the A/Anhui/1/2005(H5N1) attenuated
                          --------------------------------------------------
                          (v) favipiravir, which is primarily pursued for the treatment of influenza virus infections
                          -------------------------------------------------------------------------
                          Effectiveness of hand hygiene and provision of information in preventing influenza cases requiring hospitalization.
                          -------------------------------------------------------

                          ----------------------------------
                          I'm interested in expert panflu damage estimates
                          my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                          Comment


                          • #14
                            Re: snippets

                            2012/05/11:67258
                            -----------------------------------
                            Subtype- and antigenic site-specific differences in biophysical influences on evolution of HA
                            ------------------------------------
                            identification of pneumonia and influenza deaths using the death certificate pipeline.
                            --------------------------------------
                            asymptomatic pneumococcal carriage may also affect subsequent influenza virus infection
                            and the clinical outcome
                            ---------------------------------------
                            reassortant strains associated with human pandemic influenza H1N1 and swine
                            influenza H1N2 (SIV) viruses in swine populations in South Korea.
                            ---------------------------------------
                            Characteristics of a widespread community cluster of H275Y oseltamivir-resistant
                            A(H1N1)pdm09 influenza in Australia.
                            -------------------------------------------------------------------
                            global ordered RNA structure exists in influenza A segments 1, 5, 7 and 8,
                            ------------------------------------------------------------------------
                            Influenza. One H5N1 paper finally goes to press; second greenlighted
                            --------------------------------------------------------------
                            a Vero cell-based HPAI H5N1 vaccine candidate (H5N1/YNVa) with stable high yield was achieved
                            ------------------------------------------------------------------
                            Seasonal Trivalent Inactivated Influenza Vaccine Protects against 1918 ...Virus in Ferrets.
                            ----------------------------------------------------------
                            (v) favipiravir,
                            -------------------------------
                            67336
                            Pandemic H1N1 Influenza Infection and Vaccination in Humans Induces Cross-Protective
                            Antibodies that Target the Hemagglutinin Stem.
                            ----------------------------------
                            Low Rates of Influenza Immunization in Young Children Under Ontario's Universal Influenza
                            Immunization Program.
                            -----------------------------------
                            CD4(+) T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal
                            antigens and functionally differs from active influenza infection.
                            ..Pandemic H1N1-vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific
                            CD4(+) T cells that was more pronounced in individuals not pre-vaccinated with seasonal
                            influenza (p = 0.01). T-cell levels towards seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26).
                            Likewise, pre-vaccination with seasonal influenza induced a less pronounced increase in
                            specific antibodies.
                            ---------------------------------------
                            TiO(2) photocatalysis could be used to inactivate the influenza virus.
                            ----------------------------------
                            intercontinental reassortment in gulls.
                            -------------------------------------
                            antiviral activity of ASN2
                            single amino acid mutation at position 499 of the influenza virus PB1 protein that confers
                            resistance to ASN2,
                            ---------------------------------------
                            EcoHealth and the Influenza A/H5N1 Dual Use Issue.
                            -----------------------------------------
                            (FLU-v) targeting T cell responses to NP, M1 and M2
                            CONCLUSION: FLU-v is safe and induces a vaccine-specific cellular immunity.
                            ----------------------------------------------
                            67377, 29.May,2012
                            annual economic costs of influenza varied from $13.9 thousand to $957.5 million among
                            US counties, with a median of $2.47 million.
                            --> costs of flu ~ $7.5B per year in USA
                            --------------------------------------------------------------------
                            bovine Lactoferrin C-lobe strongly binds to the HA(2) region of viral hemagglutinin,
                            precisely the highly conserved region containing the fusion peptide.
                            inhibited virus hemagglutination
                            ------------------------------------------------------------------------
                            67465
                            ---------------------------------------------------------------------------------
                            {laninamivir in children similar to zanamivir}
                            -----------------------------------------------------------------------------------
                            H3N2 influenza A viruses have been widely circulating in human populations since the pandemic of 1968. A striking feature of the evolutionary development of this strain has been its 'canalized' nature, with narrow evolutionary trees dominated by long trunks with few branching, or bifurcation events …

                            Constrained evolution drives limited influenza diversity. {in human H3N2}
                            few branching, immunity was expected to create more more diversity
                            processive evolution down a single path
                            http://www.biomedcentral.com/1741-7007/10/38.
                            --------------------------------------------------------------------------------------
                            H5N1 in Yunnan,2009-Jul,2011 : 2.3.2 and 2.3.4, 3 (II-1 to II-3) and 2 subclades (I-1 and I-2),
                            2.3.2 II-1 and II-2 were new variant strains
                            -----------------------------------------------------------------------------------
                            67533
                            peramivir fever alleviation median 17.0 h for H3N2
                            oseltamivir, zanamivir, and laninamivir more (--> worse)
                            -------------------------------------------------------------------------
                            in animal models vaccination against H3N2 reduced immunity against H5N1
                            otherwise induced by a prior natural infection with H3N2
                            ----------------------------------------------------------------------
                            Oral administration of milk fermented with Lactococcus lactis subsp. cremoris
                            FC protects mice against influenza virus infection.
                            ----------------------------------------------------------------------------
                            67638
                            Direct transmission of avian influenza viruses to mammals has become an increasingly investigated topic during the past decade; however, isolates that have been primarily investigated are typically ones originating from human or poultry outbreaks. Currently there is minimal comparative information o …

                            Despite the lack of α2,6 linked SA binding, these AIVs productively infected both
                            the upper and lower respiratory tract of ferrets, resulting in nasal viral shedding and
                            pulmonary lesions with minimal morbidity
                            These results demonstrate that avian influenza viruses, which are endemic in aquatic birds,
                            can potentially infect humans and other mammals without adaptation
                            --------------------------------------------------
                            67685
                            Influenza viruses are characterized by an ability to cross species boundaries and evade host immunity, sometimes with devastating consequences. The 2009 pandemic of H1N1 influenza A virus highlights the importance of pigs in influenza emergence, particularly as intermediate hosts by which avian viru …

                            Evolution of an Eurasian Avian-like Influenza Virus in Na&#239;ve and Vaccinated Pigs.
                            -------------------------------------------------------------------------------
                            Infection due to 2009 pandemic H1N1 influenza A virus (A[H1N1]pdm09) is commonly manifested as mild infection but occasionally as severe pneumonia. We hypothesized that host genetic variations may contribute to disease severity. An initially small-scale genome-wide association study guided the selec …

                            CD55 gene polymorphisms are associated with severe A(H1N1)pdm09 infection
                            selection of CD55 single nucleotide polymorphisms
                            host genetic variations

                            ---------------------------------------------------------------------------------
                            An analysis was undertaken to measure age-specific vaccine effectiveness (VE) of 2010/11 trivalent seasonal influenza vaccine (TIV) and monovalent 2009 pandemic influenza vaccine (PIV) administered in 2009/2010. The test-negative case-control study design was employed based on patients consulting pr …

                            in UK , trivalent effectiveness against confirmed A(H1N1)pdm2009 was 56&#37;
                            87% <5-year , 84% 5-14year ,
                            monovalent 2009 vax was only 28% , 72% in <5
                            trivalent for confirmed B was 57% , 75% in 5-14
                            TIV provided moderate protection against the main circulating strains in 2010/2011,
                            with higher protection in children
                            -------------------------------------------------------------------
                            A remarkable debate recently arose on a global scale, about bioethics, biohazard, bioweaponry and bioterrorism issues related to scientific research concerning the induced transition of the highly lethal H5N1 avian flu virus from a non-pandemic to a tentatively pandemic strain, which might fall into …

                            ...potent modular weapon.
                            ---------------------------------------------------------
                            Incidence and risk factors for H5 highly pathogenic avian influenza
                            infection in flocks of apparently clinically healthy ducks.
                            --------------------------------------------------------
                            Dynamic of H5N1 virus in Cambodia and emergence of a novel endemic sub-clade.
                            -------------------------------------------------------
                            67952,2012.07.01
                            An Overlapping Protein-Coding Region in Influenza A Virus Segment 3 Modulates the Host Response.
                            --------------------------------
                            Effects of polyphenol compounds on influenza A virus replication and definition of their mechanism of action.
                            ------------------------------
                            Key molecular factors in HA and PB2 contribute to the efficient transmission of the 2009 H1N1 pandemic influenza virus.
                            Q226R(4),A271T(1),
                            --------------------------------------
                            Dietary Supplementation with Lacto-Wolfberry Enhances the Immune Response and Reduces Pathogenesis to Influenza Infection in Mice.
                            ---------------------------------------
                            Domestic Cats Are Susceptible to Infection With Low Pathogenic Avian Influenza Viruses From Shorebirds.
                            -------------------------------------
                            Susceptibility of different cell lines to Avian and Swine Influenza viruses.
                            -------------------------------------
                            MORTALITY ATTRIBUTABLE TO 9 COMMON INFECTIONS: significant effect of influenza A, RSV, influenza B, norovirus and parainfluenza in the elderly.
                            Influenza A and RSV were significantly associated with mortality in all studied age groups;
                            influenza B, and parainfluenza additionally in those aged 75+ years, and norovirus in those
                            aged 85+ years. The proportion of deaths attributable to seasonal viruses was:
                            6.8% (85+ years); 4.4% (75-84 years); 1.4% (65-74years)
                            ----------------------------------------
                            Can high-pathogenic avian influenza viruses with novel hemagglutinin serotypes other than H5 or H7 emerge?
                            ----------------------------------------

                            The failure of the M segment from pH1N1 to offer R1 and R2 a replication advantage in pigs over the parental strains came as a surprise because field SIV isolates with the same genotype appear to have a better fitness and are dominant in North American pig farms.
                            --------------------------------------------
                            Review of the 2011–2012 winter influenza season, northern hemisphere.
                            ----------------------------------------------
                            Low Pathogenic Avian Influenza A (H7N2) Virus Infection in Immunocompromised Adult, New York, USA, 2003.
                            -----------------------------------------
                            Human Infection from Avian-like Influenza A (H1N1) Viruses in Pigs, China.

                            A/swine/Jiangsu/40/2011
                            -------------------------------------------
                            68006, 2012.07.06
                            I'm interested in expert panflu damage estimates
                            my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

                            Comment


                            • #15
                              Re: snippets

                              68696
                              ---------------------------------------------------------------
                              AL18, an inhibitor of human cytomegalovirus DNA polymerase, was serendipitously found to
                              also block the interaction between the PB1 and PA polymerase subunits of influenza A virus.
                              Furthermore, AL18 effectively inhibited influenza A virus polymerase activity and the overall
                              replication of influenza A and B viruses. A molecular model to explain the binding of AL18 to
                              both cytomegalovirus and influenza targets is proposed. Thus, AL18 represents an interesting
                              lead for the development of new antivirals.
                              AL18, an inhibitor of human cytomegalovirus DNA polymerase, was serendipitously found to also block the interaction between the PB1 and PA polymerase subunits of influenza A virus. Furthermore, AL18 effectively inhibited influenza A virus polymerase activity and the overall replication of influenza …

                              ----------------------------------------------------------------------------------------------------------------
                              influenza evolution 2480
                              --------------------------------------------------------------------------------
                               We show that southern China is unlikely to represent an epicenter of global influenza activity, as influenza activity in Shenzhen is characterized by significant annual cycles, multiple viral introductions every year, limited persistence across epidemic seasons, and viruses that generally are not positioned on the trunk of the global influenza phylogeny.Conclusions. We propose that novel influenza viruses emerge and evolve in multiple geographic localities and that the global evolution of influenza virus is complex and does not simply originate from a southern Chinese epicenter.
                              We propose that novel influenza viruses emerge and evolve in multiple geographic localities and that the global evolution of influenza virus is complex and does not simply originate from a southern Chinese epicenter.

                              --------------------------------------------------------------------------------------
                              Replication and adaptive mutations of low pathogenic avian influenza viruses in tracheal organ cultures of different avian species.
                              -------------------------------------------------------
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                              I'm interested in expert panflu damage estimates
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