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J Virol. 2011 Nov 16. [Epub ahead of print]
Adaptation of a duck influenza A virus in quail.
Yamada S, Shinya K, Takada A, Ito T, Suzuki T, Suzuki Y, Le QM, Ebina M, Kasai N, Kida H, Horimoto T, Rivailler P, Chen LM, Donis RO, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and Immunology.
Abstract
Quails are thought to serve as intermediate hosts of influenza A viruses between aquatic birds and terrestrial birds, such as chickens, due to their high susceptibility to aquatic bird viruses, which then adapt to replicate efficiently in their new hosts. However, does replication of aquatic bird influenza viruses in quails similarly result in their efficient replication in humans? Using sialic acid-galactose linkage-specific lectins, we found both avian- (sialic acid-α2-3-galactose [Siaα2-3Gal] linkages on sialyloligosaccharides) and human-(Sisα2-6Gal) type receptors on the tracheal cells of quail, consistent with previous reports. We also passaged a duck H3N2 virus in quails 19 times. Sequence analysis revealed that eight mutations accumulated in hemagglutinin during these passages. Interestingly, many of the altered HA amino acids found in the adapted virus are present in human seasonal viruses but not in duck viruses. We also found that stepwise stalk deletion of neuraminidase occurred during passages, resulting in reduced neuraminidase function. Despite some hemagglutinin mutations near the receptor-binding pocket, appreciable changes in receptor specificity were not detected. However, reverse genetics-generated viruses that possessed the hemagglutinin and neuraminidase of the quail-passaged virus replicated significantly better than the virus possessing the parent HA and neuraminidase in normal human bronchial epithelial cells, whereas no significant difference in replication between the two viruses was observed in duck cells. Further, the quail-passaged, but not the original duck virus replicated in human bronchial epithelial cells. These data indicate that quails can serve as intermediate hosts for aquatic bird influenza viruses to transmit to humans.
PMID:
22090115
[PubMed - as supplied by publisher]
Adaptation of a duck influenza A virus in quail.
Yamada S, Shinya K, Takada A, Ito T, Suzuki T, Suzuki Y, Le QM, Ebina M, Kasai N, Kida H, Horimoto T, Rivailler P, Chen LM, Donis RO, Kawaoka Y.
Source
Division of Virology, Department of Microbiology and Immunology.
Abstract
Quails are thought to serve as intermediate hosts of influenza A viruses between aquatic birds and terrestrial birds, such as chickens, due to their high susceptibility to aquatic bird viruses, which then adapt to replicate efficiently in their new hosts. However, does replication of aquatic bird influenza viruses in quails similarly result in their efficient replication in humans? Using sialic acid-galactose linkage-specific lectins, we found both avian- (sialic acid-α2-3-galactose [Siaα2-3Gal] linkages on sialyloligosaccharides) and human-(Sisα2-6Gal) type receptors on the tracheal cells of quail, consistent with previous reports. We also passaged a duck H3N2 virus in quails 19 times. Sequence analysis revealed that eight mutations accumulated in hemagglutinin during these passages. Interestingly, many of the altered HA amino acids found in the adapted virus are present in human seasonal viruses but not in duck viruses. We also found that stepwise stalk deletion of neuraminidase occurred during passages, resulting in reduced neuraminidase function. Despite some hemagglutinin mutations near the receptor-binding pocket, appreciable changes in receptor specificity were not detected. However, reverse genetics-generated viruses that possessed the hemagglutinin and neuraminidase of the quail-passaged virus replicated significantly better than the virus possessing the parent HA and neuraminidase in normal human bronchial epithelial cells, whereas no significant difference in replication between the two viruses was observed in duck cells. Further, the quail-passaged, but not the original duck virus replicated in human bronchial epithelial cells. These data indicate that quails can serve as intermediate hosts for aquatic bird influenza viruses to transmit to humans.
PMID:
22090115
[PubMed - as supplied by publisher]
