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Rapid Diagnosis Of Influenza: An Evaluation Of Two Commercially Available Rt-Pcr Assays

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  • Rapid Diagnosis Of Influenza: An Evaluation Of Two Commercially Available Rt-Pcr Assays

    J Infect. 2012 Apr 17. [Epub ahead of print]
    Rapid Diagnosis Of Influenza: An Evaluation Of Two Commercially Available Rt-Pcr Assays.
    Li M, Brenwald N, Bonigal S, Chana K, Osman H, Oppenheim B.
    Source

    Department of Medical Microbiology, City Hospital, Birmingham, United Kingdom. , HPA West Midlands Public Health Laboratory, Birmingham Heartlands Hospital, United Kingdom.
    Abstract
    OBJECTIVE:

    To evaluate the sensitivity and specificity of influenza virus detection by two commercial reverse transcriptase PCR methods compared with a reference real time PCR.
    METHODS:

    122 clinical specimens were tested on Xpert? Flu and RealStar? Influenza Screen & Type. A reference real-time RT-PCR, at a specialist laboratory was chosen as the gold standard for comparison.
    RESULTS:

    RealStar? Influenza Screen & Type had higher sensitivity for influenza A and influenza B respectively (92.3% and 88.2%) when compared to Xpert? Flu (78.8% and 76.5%). Both tests had excellent specificity.
    CONCLUSIONS:

    The simplicity and speed of the Xpert? Flu system could allow it to be used in the near-patient setting; however in circumstances where excluding a diagnosis of influenza may be critical, negative specimens may need to be repeated using a more sensitive assay.

    Copyright ? 2012. Published by Elsevier Ltd.

    PMID:
    22522291
    [PubMed - as supplied by publisher]

    The simplicity and speed of the Xpert(®) Flu system could allow it to be used in the near-patient setting; however in circumstances where excluding a diagnosis of influenza may be critical, negative specimens may need to be repeated using a more sensitive assay.

  • #2
    Re: Rapid Diagnosis Of Influenza: An Evaluation Of Two Commercially Available Rt-Pcr Assays

    J Infect. 2012 Oct 16. pii: S0163-4453(12)00298-8. doi: 10.1016/j.jinf.2012.10.012. [Epub ahead of print]
    Response Letter to Rapid diagnosis of influenza: an evaluation of two commercially available RT-PCR assays.
    Charrel R?mi N, Nicolas S.
    Source

    Aix Marseille Univ, IRD French Institute of Research for Development, EHESP French School of Public Health, UMR_D 190 "Emergence des Pathologies Virales", 13005, Marseille, France; IHU Mediterranee Infection, APHM Public Hospitals of Marseille, 13005 Marseille, France.

    PMID:
    23085248
    [PubMed - as supplied by publisher]

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