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Science DOI: 10.1126/science.1229455
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H5N1 Hybrid Viruses Bearing 2009/H1N1 Virus Genes Transmit in Guinea Pigs by Respiratory Droplet
Ying Zhang1,*,
Qianyi Zhang1,2,*,
Huihui Kong1,
Yongping Jiang1,
Yuwei Gao1,
Guohua Deng1,
Jianzhong Shi1,
Guobin Tian1,
Liling Liu1,
Jinxiong Liu1,
Yuntao Guan1,
Zhigao Bu1,
Hualan Chen1,2,?
+ Author Affiliations
1State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People?s Republic of China.
2College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730030, People?s Republic of China.
↵?Corresponding author. E-mail: hlchen1@yahoo.com
↵* These authors contributed equally to this work.
Abstract
In the past, avian influenza viruses have crossed species? barriers to trigger human pandemics by reassorting with mammal-infective viruses in intermediate livestock hosts. H5N1 viruses are able to infect pigs, and some of them have affinity for the mammalian type α-2,6-linked sialic acid airway receptor. By using reverse genetics, we systemically created 127 reassortant viruses between a duck isolate of H5N1, specifically retaining its hemagglutinin (HA) gene throughout, and a highly transmissible, human-infective H1N1 virus. We tested the virulence of the reassortants in mice as a correlate for virulence in humans, and tested transmissibility in guinea pigs, which have both avian and mammalian types of airway receptor. Transmission study showed that both polymerase PA gene and nonstructural protein NS gene of H1N1 virus made the H5N1 virus transmissible by respiratory droplet between guinea pigs, without death. Further experiments implicated other H1N1 genes in the enhancement of mammal-to-mammal transmission, including nucleoprotein (NP), neuraminidase (NA), and matrix (M), as well as mutations in H5 HA that improve affinity for human-like airway receptors. Hence, avian H5N1 subtype viruses do have the potential to acquire mammalian transmissibility by reassortment in current agricultural scenarios.
Report
H5N1 Hybrid Viruses Bearing 2009/H1N1 Virus Genes Transmit in Guinea Pigs by Respiratory Droplet
Ying Zhang1,*,
Qianyi Zhang1,2,*,
Huihui Kong1,
Yongping Jiang1,
Yuwei Gao1,
Guohua Deng1,
Jianzhong Shi1,
Guobin Tian1,
Liling Liu1,
Jinxiong Liu1,
Yuntao Guan1,
Zhigao Bu1,
Hualan Chen1,2,?
+ Author Affiliations
1State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People?s Republic of China.
2College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730030, People?s Republic of China.
↵?Corresponding author. E-mail: hlchen1@yahoo.com
↵* These authors contributed equally to this work.
Abstract
In the past, avian influenza viruses have crossed species? barriers to trigger human pandemics by reassorting with mammal-infective viruses in intermediate livestock hosts. H5N1 viruses are able to infect pigs, and some of them have affinity for the mammalian type α-2,6-linked sialic acid airway receptor. By using reverse genetics, we systemically created 127 reassortant viruses between a duck isolate of H5N1, specifically retaining its hemagglutinin (HA) gene throughout, and a highly transmissible, human-infective H1N1 virus. We tested the virulence of the reassortants in mice as a correlate for virulence in humans, and tested transmissibility in guinea pigs, which have both avian and mammalian types of airway receptor. Transmission study showed that both polymerase PA gene and nonstructural protein NS gene of H1N1 virus made the H5N1 virus transmissible by respiratory droplet between guinea pigs, without death. Further experiments implicated other H1N1 genes in the enhancement of mammal-to-mammal transmission, including nucleoprotein (NP), neuraminidase (NA), and matrix (M), as well as mutations in H5 HA that improve affinity for human-like airway receptors. Hence, avian H5N1 subtype viruses do have the potential to acquire mammalian transmissibility by reassortment in current agricultural scenarios.
