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J Virol. Mouse dipeptidyl peptidase 4 (DPP4) is not a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection
[Source: Journal of Virology, full page: (LINK). Abstract, edited.]
Mouse dipeptidyl peptidase 4 (DPP4) is not a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection
Adam S. Cockrell a*, Kayla M. Peck d, Boyd L. Yount b, Sudhakar S. Agnihothram b, Trevor Scobey b, Nicole R. Curnes a, Ralph S. Baric b,c and Mark T. Heise a,c
Author Affiliations: Departments of Genetics<SUP>a </SUP>Epidemiology<SUP>b </SUP>Microbiology and Immunology<SUP>c </SUP>Biology<SUP>d</SUP>, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, 27599
Published ahead of print 26 February 2014, doi: 10.1128/JVI.03764-13 <CITE>JVI.03764-13 </CITE>
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<CITE></CITE>ABSTRACT
Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for MERS-CoV infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse adapted MERS-CoV for rapid assessment of therapeutics.
FOOTNOTES
*Corresponding Author: University of North Carolina, Department of Genetics, Burnett-Womack Bldg., CB#7292, Chapel Hill, NC, 27599, adam_cockrell@unc.edu, 919-966-4026
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
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Mouse dipeptidyl peptidase 4 (DPP4) is not a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV) infection
Adam S. Cockrell a*, Kayla M. Peck d, Boyd L. Yount b, Sudhakar S. Agnihothram b, Trevor Scobey b, Nicole R. Curnes a, Ralph S. Baric b,c and Mark T. Heise a,c
Author Affiliations: Departments of Genetics<SUP>a </SUP>Epidemiology<SUP>b </SUP>Microbiology and Immunology<SUP>c </SUP>Biology<SUP>d</SUP>, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, 27599
Published ahead of print 26 February 2014, doi: 10.1128/JVI.03764-13 <CITE>JVI.03764-13 </CITE>
<CITE></CITE>
<CITE></CITE>
<CITE></CITE>ABSTRACT
Human dipeptidyl peptidase 4 (hDPP4) was recently identified as the receptor for MERS-CoV infection, suggesting that other mammalian DPP4 orthologs may also support infection. We demonstrate that mouse DPP4 cannot support MERS-CoV infection. However, employing mouse DPP4 as a scaffold we identified two critical amino acids (A288L and T330R) that regulate species specificity in the mouse. This knowledge can support the rational design of a mouse adapted MERS-CoV for rapid assessment of therapeutics.
FOOTNOTES
*Corresponding Author: University of North Carolina, Department of Genetics, Burnett-Womack Bldg., CB#7292, Chapel Hill, NC, 27599, adam_cockrell@unc.edu, 919-966-4026
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
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