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Recombination possibility - My Question to Niman in sequences lingo...
But for a single mutation Q226L, recombination with NP would
probably not
be required. We see a few such point-mutations in almost every human H5N1 isolate. And these mutations usually differ in different isolates.
So, considering the large amount of viruses in a host, I assume
that hundreds or thousands of single-point mutations are "tested"
in one infection. Most will be less viable, but if Q226L were more
viable, I think it has a good chance to evolve by "normal"
methods.
This may include short recombinations with NP or whatever,
which has the effect of just one nucleotide changing.
Random muations are rare. They are not "normal". They are largely nonsense.
The haemagglutinin glycoprotein HA of influenza viruses is responsible for the attachment of the virus to neuraminic acid-containing receptors at the cell surface and subsequent penetration by triggering fusion of the viral envelope with cellular membranes. To express full activity of the newly synt …
Nature. 1989 Jul 13;340(6229):156-7
Khatchikian D, Orlich M, Rott R.
The haemagglutinin glycoprotein HA of influenza viruses is responsible for the attachment of the virus to neuraminic acid-containing receptors at the cell surface and subsequent penetration by triggering fusion of the viral envelope with cellular membranes. To express full activity of the newly synthesized precursor, HA has to be modified by post-translational proteolytic cleavage into the polypeptides HA1 and HA2 by cellular enzymes. If proteases suitable for cleavage are not present in the host cell, the resulting virus particles are non-infectious. During adaptation of the apathogenic influenza virus A/turkey/Oregon/71 to chicken embryo cells, which are not permissive for HA cleavage, we obtained an infectious virus variant with increased pathogenicity. Sequence analysis revealed that during adaptation 54 nucleotides were inserted into the HA gene; their sequence corresponds to a region of the 28S ribosomal RNA (= Host RNA). This insertion is probably responsible for increased cleavability of HA, as well as for infectivity and pathogenicity of the adapted virus.
Re: Recombination possibility - My Question to Niman in sequences lingo...
My My, Ganseerpeel you find an outstanding example. In this case the recombination came from the host ribosomal RNA. Nature is full of resources.
You whant something else...
This week while I wasw occuied with PRRSv sequencing ( swine virus ) I had the Idea to probe some of their polymorphism into los alamos flu data bank just to see.
Most of the time the polymorphism did not find matchs with influenza, but when a match was done most of the time it was a travel log including many swines sequences... and only of north american or asiatic sequences ... the north american type of PRRSV is not present in europe.
In the diagnostic routine, it is not rare to see PRRSV & SIV positives swines. Both of theses virus are RNA virus.
Re: Recombination possibility - My Question to Niman in sequences lingo...
Originally posted by Mingus
My My, Ganseerpeel you find an outstanding example. In this case the recombination came from the host ribosomal RNA. Nature is full of resources.
You whant something else...
This week while I wasw occuied with PRRSv sequencing ( swine virus ) I had the Idea to probe some of their polymorphism into los alamos flu data bank just to see.
Most of the time the polymorphism did not find matchs with influenza, but when a match was done most of the time it was a travel log including many swines sequences... and only of north american or asiatic sequences ... the north american type of PRRSV is not present in europe.
In the diagnostic routine, it is not rare to see PRRSV & SIV positives swines. Both of theses virus are RNA virus.
Are theses inter-virus recombination frequent ?
I had previously noted a 18 BP region of identity between flu and Ebola
Re: Recombination possibility - My Question to Niman in sequences lingo...
Ebola is rare, where I live, but what about Rhinoviruses ?
They are very common.
How many segements ? Are sequences available ?
Can H5N1 combine with Rhinoviruses (or other cold-viruses)
to improve H2H transmissiability ?
Re: Recombination possibility - My Question to Niman in sequences lingo...
Originally posted by gsgs
Ebola is rare, where I live, but what about Rhinoviruses ?
They are very common.
How many segements ? Are sequences available ?
Can H5N1 combine with Rhinoviruses (or other cold-viruses)
to improve H2H transmissiability ?
The is much more homology between avian and seasonal flu than other viruses. It's HOMOLOGOPUS recombination (remember the landing zones), and avian flu frequently acquires mammalian polymorphism from swine or human flu, including influenza B.
Re: Recombination possibility - My Question to Niman in sequences lingo...
this thread is about non-homologuous recombination however,
and apparantly it does also occur.
And double infection with a cold is much more likely.
I'd like to see the sequences of one cold-virus, where can I find it ?
Also PRRSV.
Re: Recombination possibility - My Question to Niman in sequences lingo...
Ok I will post some examples of at least 10nucleotide match with wild north american PRRS virus sequences we have here in our private PRRSv sequences bank.
The sequences legally owned to the veterinary who pay for them so I won't post them.
Just the polymorphisms are ok.
Note that PRRSv is a swine virus and is not know to infect others species.
Re: Recombination possibility - My Question to Niman in sequences lingo...
Originally posted by gsgs
this thread is about non-homologuous recombination however,
and apparantly it does also occur.
And double infection with a cold is much more likely.
I'd like to see the sequences of one cold-virus, where can I find it ?
Also PRRSV.
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