Announcement

Collapse
No announcement yet.

H5N1 in flies in Japan

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • H5N1 in flies in Japan

    This week, a poster here at FT have ask a question about the sequence A/blow fly/Kyoto/93/2004 wich is recorded in GenBank since a long time.

    Surprisingly the related article have been release today !

    Here it is.

    ___________________________
    During the outbreak of highly pathogenic avian influenza that occurred in Tamba Town, Kyoto Prefecture in 2004, a total of 926 flies were collected from six sites within a radius of 2.3 km from the poultry farm. The H5 influenza A virus genes were detected from the intestinal organs, crop, and gut o …


    1: Am J Trop Med Hyg. 2006 Aug;75(2):327-332.Click here to read Links
    DETECTION AND ISOLATION OF HIGHLY PATHOGENIC H5N1 AVIAN INFLUENZA A VIRUSES FROM BLOW FLIES COLLECTED IN THE VICINITY OF AN INFECTED POULTRY FARM IN KYOTO, JAPAN, 2004.

    * Sawabe K,
    * Hoshino K,
    * Isawa H,
    * Sasaki T,
    * Hayashi T,
    * Tsuda Y,
    * Kurahashi H,
    * Tanabayashi K,
    * Hotta A,
    * Saito T,
    * Yamada A,
    * Kobayashi M.

    Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan; Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan; Department of Virology III, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.

    During the outbreak of highly pathogenic avian influenza that occurred in Tamba Town, Kyoto Prefecture in 2004, a total of 926 flies were collected from six sites within a radius of 2.3 km from the poultry farm. The H5 influenza A virus genes were detected from the intestinal organs, crop, and gut of the two blow fly species, Calliphora nigribarbis and Aldrichina grahami, by reverse transcription-polymerase chain reaction for the matrix protein (M) and hemagglutinin (HA) genes. The HA gene encoding multiple basic amino acids at the HA cleavage site indicated that this virus is a highly pathogenic strain. Based on the full-length sequences of the M, HA, and neuraminidase (NA) segments of virus isolates through embryonated chicken eggs, the virus from C. nigribarbis (A/blow fly/Kyoto/93/2004) was characterized as H5N1 subtype influenza A virus and shown to have > 99.9% identities in all three RNA segments to a strain from chickens (A/chicken/Kyoto/3/2004) and crows (A/crows/Kyoto/53/2004) derived during this outbreak period in Kyoto in 2004. Our results suggest it is possible that blow flies could become a mechanical transmitter of H5N1 influenza virus.

    PMID: 16896143 [PubMed - as supplied by publisher]

  • #2
    Re: H5N1 in flies in Japan

    I was hoping that was some sort of mistake, but it looks like that's not the case. Aren't blowflies one of the first insects drawn to a corpse?

    I hope they aren't among the flies that bite people.
    Does anyone know if mosquitos visit dead animals for blood?

    Just thinking out loud so please forgive me if my questions are stupid.
    "There's a chance peace will come in your life - please buy one" - Melanie Safka
    "The greatest way to live with honor in this world is to be what we pretend to be" - Socrates

    Comment


    • #3
      Re: H5N1 in flies in Japan

      Mosquitos are drawn to the carbon dioxide that is expelled from the body when one exhales. Dead bodies do not exhale.

      Mingus - Do you think it is a coincidence?

      Comment


      • #4
        Re: H5N1 in flies in Japan

        <table class="tborder" id="post5002" align="center" border="0" cellpadding="6" cellspacing="1" width="100%"><tbody><tr><td class="thead"> 03-20-2006, 06:21 AM <!-- / status icon and date -->
        </td> </tr> <tr> <td class="alt2" style="padding: 0px;"> <!-- user info --> <table border="0" cellpadding="0" cellspacing="6" width="100%"> <tbody><tr> <td class="alt2"></td> <td nowrap="nowrap"> Snowy Owl <script type="text/javascript"> vbmenu_register("postmenu_5002", true); </script>
        Director - Vice President
        </td> <td width="100%"> </td> <td nowrap="nowrap" valign="top"> Join Date: Feb 2006
        Location: South of the North P?le
        Posts: 2,578


        </td> </tr> </tbody></table> <!-- / user info --> </td> </tr> <tr> <td class="alt1" id="td_post_5002"> <!-- message, attachments, sig --> <!-- icon and title --> H5N1 avian influenza A viruses from blow flies
        <hr style="color: rgb(204, 204, 204);" size="1"> <!-- / icon and title --> <!-- message --> Detection and Isolation of highly pathogenic
        H5N1 avian influenza A viruses from blow flies collected
        in the vicinity of an infected poultry farm in Kyoto, Japan, 2004

        LOCUS BAE47132 449 aa linear VRL 28-OCT-2005
        DEFINITION neuraminidase [Influenza A virus (A/blow fly/Kyoto/93/2004(H5N1))].
        ACCESSION BAE47132
        VERSION BAE47132.1 GI:78210827
        DBSOURCE accession AB212650.1
        KEYWORDS .
        SOURCE Influenza A virus (A/blow fly/Kyoto/93/2004(H5N1))
        ORGANISM Influenza A virus (A/blow fly/Kyoto/93/2004(H5N1))
        Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
        Influenzavirus A.
        REFERENCE 1
        AUTHORS Sawabe,K., Hoshino,K., Isawa,H., Sasaki,T., Hayashi,T., Tsuda,Y.,
        Kurahashi,H., Tanabayashi,K., Hotta,A., Saito,T., Yamada,A. and
        Kobayashi,M.
        TITLE Detection and Isolation of highly pathogenic H5N1 avian influenza A
        viruses from blow flies collected in the vicinity of an infected
        poultry farm in Kyoto, Japan, 2004
        JOURNAL Unpublished
        REFERENCE 2 (residues 1 to 449)
        AUTHORS Sawabe,K., Hoshino,K., Isawa,H. and Sasaki,T.
        TITLE Direct Submission
        JOURNAL Submitted (28-APR-2005) Kyoko Sawabe, National Institute of
        Infectious Diseases, Department of Medical Entomology; Toyama
        1-23-1, Shinjuku-ku, Tokyo, 162-8640, Japan
        (E-mail:sawabe@nih.go.jp, Tel:81-3-5285-1111(ex.2423),
        Fax:81-3-5285-1147)
        FEATURES Location/Qualifiers
        source 1..449
        /organism="Influenza A virus (A/blow
        fly/Kyoto/93/2004(H5N1))"
        /strain="A/blow fly/Kyoto/93/2004(H5N1)"
        /serotype="H5N1"
        /isolation_source="blow fly"
        /db_xref="taxon:327048"
        /segment="6"
        /country="Japan: Kyoto, Tamba"
        /note="isolated from blow fly in Kyoto, 2004"
        Protein 1..449
        /product="neuraminidase"
        Region 82..414
        /region_name="Sialidases or neuraminidases function to
        bind and hydrolyze terminal sialic acid residues from
        various glycoconjugates as well as playing roles in
        pathogenesis, bacterial nutrition and cellular
        interactions"
        /note="Sialidase"
        /db_xref="CDD:29333"
        CDS 1..449
        /gene="NA"
        /coded_by="AB212650.1:21..1370"
        ORIGIN
        1 mnpnqkiiti gsicmvigiv slmlqvgnmi siwvshsiqt gnqrqaepis ntkfltekav
        61 tsvtlagnss lcpisgwavh skdnsirigs kgdvfvirep fiscshlecr tffltqgall
        121 ndkhsngtvk drsphrtlms cpvgeapspy nsrfesvaws asachdgtsw ltigisgpdn
        181 gavavlkyng iitdtikswr nnilrtqese cacvngscft vmtdgpsngq asykifkmek
        241 gkvvksveld apnyhyeecs cypdageitc vcrdnwhgsn rpwvsfnqdl eyqigyicsg
        301 vfgdnprpnd gtgscgpvsp ngaygvkgfs fkygngvwig rtkstnsrsg femiwdpngw
        361 tgtdssfsvk qdivaitdws gysgsfvqhp eltgldcirp cfwvelirgr pkestiwtsg
        421 ssisfcgvns dtvgwswpdg aelpftidk
        http://www.ncbi.nlm.nih.gov/entrez/v...n&val=78210827

        Blow-fly

        From Wikipedia, the free encyclopedia

        (Redirected from Blowfly)
        Jump to: navigation, search
        <!-- start content --> <table style="margin: 0pt 0pt 0.5em 1em; position: relative; border-collapse: collapse; float: right; clear: right; width: 200px;" border="1" cellpadding="0"> <tbody><tr style="text-align: center;"> <th style="background: pink none repeat scroll 0% 50%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"> ?
        Blow-fly</th> </tr> <tr> <td>
        <small>Blowfly - Genus Calliphora</small>
        </td> </tr> <tr style="text-align: center;"> <th style="background: pink none repeat scroll 0% 50%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;">Scientific classification</th> </tr> <tr style="text-align: center;"> <td> <table style="margin: 0pt auto; background: transparent none repeat scroll 0% 50%; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;" cellpadding="2"> <tbody><tr valign="top"> <td>Kingdom:</td> <td>Animalia
        </td> </tr> <tr valign="top"> <td>Phylum:</td> <td>Arthropoda
        </td> </tr> <tr valign="top"> <td>Class:</td> <td>Insecta
        </td> </tr> <tr valign="top"> <td>Order:</td> <td>Diptera
        </td> </tr> <tr valign="top"> <td>Suborder:</td> <td>Brachycera
        </td> </tr> <tr valign="top"> <td>Infraorder:</td> <td>Muscomorpha
        </td> </tr> <tr valign="top"> <td>Family:</td> <td>Calliphoridae
        </td> </tr> <tr> <td>
        </td> </tr> </tbody></table> </td> </tr> <tr> <td style="text-align: center;">
        </td> </tr> <tr bgcolor="pink"> <th> Subfamilies
        </th> </tr> <tr> <td style="padding: 0pt 0.5em;"><table cellpadding="0" cellspacing="0"> <tbody><tr> <td>
        </td> </tr> </tbody></table> </td> </tr> </tbody> </table> Blow-flies (also frequently spelled blow flies or blowflies) are members of the family Calliphoridae of flies (Diptera). Flies in this family are often metallic in appearance.
        Some members of this family are known as bluebottles, clusterflies, greenbottles, and (in Britain and Australia) as blue-arsed flies. The name blow-fly comes from the fact that recently deceased corpses would swell up from bacterial decomposition in the intestinal tract, causing them to become bloated, or "fly-blown". Blow-flies are usually the first insect to come in contact with a dead animal.


        <table align="center" bgcolor="#ffffff" border="0" cellpadding="0" cellspacing="2" width="601"> <tbody><tr><td align="center">
        </td></tr><tr><td>http://en.wikipedia.org/wiki/Blowfly
        </td></tr></tbody> </table>
        <!-- / message --> <!-- sig --> __________________
        We must learn to reawaken and keep ourselves awake, not by mechanical aid, but by an infinite expectation of the dawn.
        (
        Henry David Thoreau)

        "Cure rarely, Comfort mostly, but Console always''.Hippocrates
        <!-- / sig -->
        <!-- controls --> <!-- / controls -->
        <!-- message, attachments, sig --> </td> </tr> </tbody></table> <!-- post 5002 popup menu --> <table border="0" cellpadding="4" cellspacing="1"> <tbody><tr> <td class="thead">Snowy Owl
        </td> </tr> <tr><td class="vbmenu_option">View Public Profile
        </td></tr> <tr><td class="vbmenu_option">Send a private message to Snowy Owl
        </td></tr> <tr><td class="vbmenu_option">Find all posts by Snowy Owl
        </td></tr> <tr><td class="vbmenu_option">Add Snowy Owl to Your Buddy List
        </td></tr> </tbody></table>
        <!-- / post 5002 popup menu --><!-- / close content container --><!-- / post #5002 --><!-- post #5064 --> <!-- open content container -->
        <!-- this is not the last post shown on the page --> <table class="tborder" id="post5064" align="center" border="0" cellpadding="6" cellspacing="1" width="100%"> <tbody><tr> <td class="thead"> #2 <input name="plist[5064]" id="plist_5064" style="margin: 0px 0px 0px 5px; padding: 0px; vertical-align: middle;" value="0" onclick="inlineMod.toggle(this)" type="checkbox">
        <!-- status icon and date --> 03-20-2006, 02:57 PM <!-- / status icon and date -->
        </td> </tr> <tr> <td class="alt2" style="padding: 0px;"> <!-- user info --> <table border="0" cellpadding="0" cellspacing="6" width="100%"> <tbody><tr> <td nowrap="nowrap"> emmysue <script type="text/javascript"> vbmenu_register("postmenu_5064", true); </script>
        Valued Member
        </td> <td width="100%"> </td> <td nowrap="nowrap" valign="top"> Join Date: Feb 2006
        Posts: 92


        </td> </tr> </tbody></table> <!-- / user info --> </td> </tr> <tr> <td class="alt1" id="td_post_5064"> <!-- message, attachments, sig --> <!-- icon and title --> Re: H5N1 avian influenza A viruses from blow flies
        <hr style="color: rgb(204, 204, 204);" size="1"> <!-- / icon and title --> <!-- message --> Does this suggest h5n1 can be transmitted via insect-vector???
        <!-- / message -->
        <!-- controls --> <!-- / controls -->
        <!-- message, attachments, sig --> </td> </tr> </tbody></table> <!-- post 5064 popup menu --> <table border="0" cellpadding="4" cellspacing="1"> <tbody><tr> <td class="thead">emmysue
        </td> </tr> <tr><td class="vbmenu_option">View Public Profile
        </td></tr> <tr><td class="vbmenu_option">Send a private message to emmysue
        </td></tr> <tr><td class="vbmenu_option">Send email to emmysue
        </td></tr> <tr><td class="vbmenu_option">Find all posts by emmysue
        </td></tr> <tr><td class="vbmenu_option">Add emmysue to Your Buddy List
        </td></tr> </tbody></table>
        <!-- / post 5064 popup menu -->

        <!-- / close content container --><!-- / post #5064 --><!-- post #5070 --> <!-- open content container --> <table class="tborder" id="post5070" align="center" border="0" cellpadding="6" cellspacing="1" width="100%"><tbody><tr> <td class="thead"> #3 <input name="plist[5070]" id="plist_5070" style="margin: 0px 0px 0px 5px; padding: 0px; vertical-align: middle;" value="0" onclick="inlineMod.toggle(this)" type="checkbox">
        <!-- status icon and date --> 03-20-2006, 03:56 PM <!-- / status icon and date -->
        </td> </tr> <tr> <td class="alt2" style="padding: 0px;"> <!-- user info --> <table border="0" cellpadding="0" cellspacing="6" width="100%"> <tbody><tr> <td class="alt2"></td> <td nowrap="nowrap"> Snowy Owl <script type="text/javascript"> vbmenu_register("postmenu_5070", true); </script>
        Director - Vice President
        </td> <td width="100%"> </td> <td nowrap="nowrap" valign="top"> Join Date: Feb 2006
        Location: South of the North P?le
        Posts: 2,578


        </td> </tr> </tbody></table> <!-- / user info --> </td> </tr> <tr> <td class="alt1" id="td_post_5070"> <!-- message, attachments, sig --> <!-- icon and title --> Mechanical transmission of porcine reproductive and respiratory syndrome virus by mos
        <hr style="color: rgb(204, 204, 204);" size="1"> <!-- / icon and title --> <!-- message --> <table border="0" cellpadding="0" cellspacing="3" width="100%"> <tbody><tr><td valign="top">Mechanical transmission of porcine reproductive and respiratory syndrome virus by mosquitoes, Aedes vexans (Meigen)
        Satoshi Otake, Scott A. Dee, Kurt D. Rossow, Roger D. Moon, and Carlos Pijoan
        Swine Disease Eradication Center, University of Minnesota College of Veterinary Medicine, St. Paul, Minnesota 55108, USA (Otake, Dee, Pijoan); University of Minnesota Veterinary Diagnostic Laboratory, St. Paul, Minnesota 55108, USA (Rossow); Department of Entomology, University of Minnesota, St. Paul, Minnesota 55108, USA (Moon).

        This article has been corrected. See Can J Vet Res. 2002 October; 66(4): 294.
        This article has been cited by other articles in PMC.


        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>Abstract
        The objective of this study was to determine whether porcine reproductive and respiratory syndrome virus (PRRSV) could be transmitted to na?ve pigs by mosquitoes following feeding on infected pigs. During each of 4 replicates, mosquito-to-pig contact took place on days 5, 6, and 7 after PRRSV infection of the donor pig. A total of 300 mosquitoes [Aedes vexans (Meigen)] were allowed to feed on each viremic donor pig, housed in an isolation room. After 30 to 60 s, feeding was interrupted, and the mosquitoes were manually transferred in small plastic vials and allowed to feed to repletion on a na?ve recipient pig housed in another isolation room. Prior to contact with the recipient pig, the mosquitoes were transferred to clean vials. Swabs were collected from the exterior surface of all vials, pooled, and tested for PRRSV. Separate personnel handled the donor pig, the recipient pig, and the vial-transfer procedure. Transmission of PRRSV from the donor to the recipient pig occurred in 2 out of 4 replicates. The PRRSV isolated from the infected recipient pigs was nucleic-acid-sequenced and found to be 100% homologous with the virus used to infect the donor pigs. Homogenates of mosquito tissues collected in all replicates were positive by either polymerase chain reaction or swine bioassay. All control pigs remained PRRSV negative, and PRRSV was not detected on the surface of the vials. This study indicates that mosquitoes (A. vexans) can serve as mechanical vectors of PRRSV.

        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>Introduction
        Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically significant pathogen in the global swine industry (1). Introduction of PRRSV into na?ve herds can occur through infected pigs, semen, contaminated needles, fomites (coveralls and boots), and hands of personnel (2,3,4,5). In order to reduce the risk of the entry of PRRSV into na?ve swine populations, swine producers use stringent measures to enhance the biosecurity of their farms; however, infection of na?ve herds frequently occurs through unidentified routes. Potential routes that have not been explored include insects. Insects have long been known to serve as mechanical or biologic vectors for certain swine pathogens (6,7,8,9). Currently used methods of biosecurity, such as shower in/out, personnel downtime (no swine contact for 48 to 72 h before entering the farm), quarantine and testing of incoming stock, and bird-proofing of facilities, do not regulate the entry of insects into swine herds. Since PRRSV infection results in prolonged viremia in infected pigs (10), and since bloodborne transmission of PRRSV by contaminated needles has been proven (4), it was hypothesized that insects, particularly hematophagous species such as mosquitoes, may be vectors of PRRSV. Additionally, mosquitoes are commonly observed in swine farms during the summer and are ubiquitous in tropical pig-raising regions.
        Recently we documented that mosquitoes (Aedes spp.) can harbor homologous, infectious PRRSV following feeding upon viremic pigs raised under commercial farm conditions (11). During this investigation, approximately 550 mosquitoes were collected from a farm experiencing an acute outbreak of PRRS. Mosquitoes were pooled (30 mosquitoes/pool), and PRRSV nucleic acid was detected by polymerase chain reaction (PCR) in 1 out of 22 mosquito pools. The open reading frame (ORF) 5 region of the nucleic acid was sequenced and found to be 100% homologous with the same region in the PRRSV isolated from pigs during the acute outbreak. The supernatant from the PCR-positive sample was tested by swine bioassay and was also found to contain infectious PRRSV. Following inoculation, the bioassay pig produced PRRSV antibodies, as detected by enzyme-linked immunosorbent assay (ELISA), and the serum was positive for PRRSV by PCR and virus isolation (VI). Nucleic acid sequencing of the ORF 5 region of the PRRSV isolate's nucleic acid indicated 100% homology with the PRRSV previously recovered from the commercial swine and the mosquito homogenate.
        Although the results from that investigation indicated that homologous infectious PRRSV could be transferred from viremic pigs to mosquitoes, they did not prove that mosquitoes can transmit PRRSV from infected to na?ve pigs. Therefore, we conducted a study to evaluate whether PRRSV could be transmitted to na?ve pigs by mosquitoes following feeding on infected pigs under experimental conditions.

        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>Materials and methods
        Infection model
        Twelve 6-wk-old pigs were purchased from a farm known to be PRRSV negative on the basis of 7 years of diagnostic data and the absence of clinical signs of PRRS. The study design consisted of 4 replicates, each replicate involving 3 pigs. Each pig was housed in a separate room at the isolation facility of the University of Minnesota College of Veterinary Medicine. This facility consisted of a series of rooms that were ventilated separately and contained individual slurry pits, which prevented cross-contamination of pathogens between rooms. Upon arrival at the facility, all animals were blood-tested to ensure a PRRSV-na?ve status. During the study period, all pigs were cared for under the guidelines of University of Minnesota Institutional Animal Care policies. To initiate each replicate, 1 pig was intranasally inoculated with 5 mL of cell culture fluid containing a total dose of 10<sup>4</sup> TCID<sub>50</sub> of a field isolate (MN-30100) of PRRSV (12). The inoculated pig was designated as the donor pig. The remaining 2 pigs were not inoculated and served as the recipient pig and the protocol-control pig, respectively.Source of mosquitoes
        Mosquitoes were trapped (indoors and outdoors) at a PRRSV-positive commercial swine farm in Minnesota, using a CO<sub>2</sub>-baited CDC light trap (13). The PRRSV strain on the farm had been previously determined to be 100% homologous with the strain used to infect the donor pigs in this study. Mosquitoes were collected 3 d prior to the initiation of each replicate. They were placed into humidified incubation cages at 27?C and kept alive with sucrose solution. Then, 50 randomly sampled mosquitoes (approximately 1% of the population collected) were classified by genus and species and tested to ensure the absence of PRRSV.Mosquito-transmission protocols
        Mosquito-to-pig contact took place on days 5, 6, and 7 after inoculation of the donor pig. This period was selected on the basis of both published data and our experience indicating that the peak of PRRSV viremia in nursery-age pigs occurred at this time (4,5,10). To attempt transmission of PRRSV by mosquitoes from the donor to the recipient pig, a manual vector-transmission protocol was applied (14) (Figure 1). This method had previously been used to study transmission of bovine leukosis virus from infected to susceptible cattle by stable flies (Stomoxys calcitrans). The pigs were consciously restrained in a Panepinto sling (Asset Inventory project, University of Minnesota, St. Paul, Minnesota, USA), and transparent plastic screw-cap vials (3 cm in diameter, 5 cm in height), each containing an individual mosquito, were fastened with masking tape (Highland 2600 tape; 3M Company, St. Paul, Minnesota, USA) over the dorsal surface of the pig. The vials had on the bottom a nylon screen with 64 holes per cm<sup>2</sup>, each hole being 1 mm in diameter, which allowed the mosquito access to the pig's skin. During each replicate, 300 mosquitoes, 100 per day, were allowed to feed for 30 to 60 s on the donor pig. Non-feeding mosquitoes were discarded and not included in the daily count. Following the observation of insertion of the mosquito proboscis and initiation of abdominal swelling, feeding was interrupted by removing the vial from the back of the pig. The vials were transferred to a neutral site and the mosquitoes transferred to new, alcohol-disinfected vials prior to delivery to the recipient pig's room. The neutral transfer area was separate from the donor and recipient isolation rooms, and the vial transfer procedure took approximately 30 s per vial. The person carrying out the transfer did not contact the donor pig or the recipient pig at any time. Immediately following transfer of the vials to the recipient pig's room, the mosquitoes were allowed to feed to repletion on the recipient pig. Following the cessation of feeding, vials containing blood-fed mosquitoes were immediately placed on dry ice and submitted for PRRSV testing.
        Extensive effort was made to rule out the possibility of non-mosquito transmission of PRRSV. To ensure that the vials that contacted the recipient pig were not contaminated with PRRSV from the donor pig, swabs (Dacron fiber-tipped plastic applicator swabs; Fisher Scientific Company, Hanover Park, Illinois, USA) were collected from the exterior surface of the vials containing the mosquitoes that contacted the recipient pig and tested for PRRSV. Additionally, on days 5, 6, and 7 after infection of the donor pig in each replicate, a protocol-control procedure was conducted to determine if contamination could occur by means of the transfer process. The identical procedure of transferring vials from the donor to the recipient pig was carried out; however, the vials were empty, and the negative-control pig, housed in a separate room, was used. This procedure was conducted prior to any contact with the donor pig or the recipient pig.
        Sampling and diagnostic analysis
        During each replicate, the donor pig was bled on each exposure day (days 5, 6, and 7 postinfection) to document PRRSV viremia. Blood was collected from the recipient pig on days 7, 14, and 21 postexposure to monitor its PRRSV-infectious status. Day 5 after infection of the donor pig was equal to day 0 after exposure of the recipient pig. The protocol-control pig was bled at the beginning and the end of each replicate. All blood-fed mosquitoes collected on each exposure day were pooled in sterile tubes (BD Falcon; Becton Dickinson and Company, Franklin Lakes, New Jersey, USA) containing 3 mL of minimum essential medium (MEM). The pooled mosquitoes were compressed against the tube wall with sterile swabs and centrifuged at 6500 rpm for 5 min, then the supernatant was tested for PRRSV. Swabs from the exterior surface of the vials that contacted the recipient pig were immediately placed into sterile tubes containing 3 mL of MEM, pooled according to exposure day, and tested for PRRSV.All serum samples from the pigs, mosquito homogenates, and swabs from the vials were tested twice for PRRSV nucleic acid by reverse transcriptase PCR (RT-PCR), with the TaqMan RT-PCR assay (Perkin-Elmer Applied Biosystems, Foster City, California, USA) (15). For samples to be considered positive, PRRSV nucleic acid had to be detected in both tests. Samples with only 1 positive reaction were considered suspect. All samples were tested for viable PRRSV by VI with the use of MARC-145 cells and porcine alveolar macrophages (16). Samples from the recipient pigs and the mosquito homogenates that were positive for PRRSV by PCR or VI were nucleic-acid-sequenced to compare the ORF 5 region with that of the virus used to infect the donor pig (17). Serum was tested for PRRSV antibodies by the IDEXX ELISA (IDEXX Laboratories, Westbrook, Maine, USA) (18). Mosquito homogenates and swabs collected from the vials that were not positive by either PCR or VI were pooled by replicate and tested for the presence of infectious PRRSV by swine bioassay (7).

        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>Results
        All the animals were determined to be seronegative for PRRSV by ELISA prior to the initiation of each replicate, and PRRSV was not detected by PCR, VI, or swine bioassay in randomly sampled mosquitoes from the swine farm. The majority (96%) of the mosquitoes collected from the farm were classified as A. vexans (Meigen). Diagnostic data for the donor pigs, the recipient pigs, and the mosquito homogenates are summarized in Table I. PRRSV viremia was detected in the donor pig on each exposure day (days 5, 6, and 7 postinfection) during all 4 replicates by PCR and VI. Transmission of PRRSV from the donor to the recipient pig was demonstrated in replicates 3 and 4: the recipient pigs in replicates 3 and 4 were positive by PCR and VI on day 7 postexposure and by ELISA on day 14. PRRSV isolated from the recipient pig in replicates 3 and 4 was 100% homologous (by sequencing of the ORF 5 region of the nucleic acid) with the virus used to infect the donor pig.
        The mosquito homogenate from replicate 4 was PCR positive, and nucleic-acid-sequencing of the ORF 5 region of the virus from this sample indicated 100% homology with the virus used to infect the donor pig. Homogenates from replicates 1 and 2 were PCR suspect but positive by swine bioassay. The homogenate from replicate 3 was PCR negative; however, it too was positive by swine bioassay. The ORF 5 region of the nucleic acid of the virus isolated by swine bioassay in replicates 1, 2, and 3 was 100% homologous with that of the virus used to infect the donor pig. All the homogenates were negative by VI. All swabs collected from the exterior surface of the transfer vials were negative by PCR, VI, and swine bioassay. The protocol-control pigs remained negative by PCR, VI, and ELISA during the entire study.

        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>Discussion
        This is the first report documenting transmission of PRRSV by mosquitoes under experimental conditions. Our data clearly demonstrate that PRRSV can be mechanically transmitted to na?ve pig by mosquitoes (A. vexans) with a manual vector-transmission protocol. These results support our previous recovery of PRRSV from mosquitoes that were allowed to feed on PRRSV-infected commercial swine (11) and indicate that mosquitoes (A. vexans) could contribute to horizontal transmission of PRRSV among pigs within infected commercial farms. Although it is not known whether A. vexans and other mosquitoes can serve as a source of PRRSV transmission between farms, their capacity for traveling extended distances (2.5 to 10 km) and for multiple blood-feedings in their life (19), along with their ability to harbor and transmit PRRSV, may support this theory. PRRSV nucleic acid was detected by PCR in the mosquito homogenate from replicate 4, and the homogenates from replicates 1 and 2 were PCR suspect. The homogenate from replicate 3 was negative by PCR and VI, yet positive by swine bioassay. Although the PCR assay used in this study (TaqMan RT-PCR) is highly sensitive, its inability to detect PRRSV in mosquito homogenates must be considered and has been hypothesized to be due to inhibition by the presence of residual heme pigments or other impurities found in insects (20). We attempted to overcome this problem through the use of multiple diagnostic tests, including swine bioassay, to enhance the accuracy of our results.
        During the design of the study, a perceived risk involved in the manual vector-transmission protocol was the possibility of PRRSV infection of the recipient pigs by contaminated personnel, fomites, or transfer vials. We attempted to minimize this risk by using designated personnel, a protocol-control procedure, and the testing of transfer vials for residual PRRSV. During each replicate, separate personnel were designated to handle the donor pig, the recipient pig, or the vial-transfer procedure. All swabs collected from the exterior surface of the vials that contacted the recipient pigs were PRRSV negative by PCR, VI, and swine bioassay. The fact that the protocol-control pigs remained negative throughout the study suggests that the transfer protocol was not a mechanical means of contamination.
        Although we demonstrated transmission in 2 out of 4 replicates, this study was not designed to measure the frequency of the observed events, and the quantity of PRRSV within an individual mosquito was not calculated. Therefore, no conclusions can be drawn regarding the probability of transmission of PRRSV by an individual mosquito, the minimum number of mosquitoes required to infect a na?ve pig, or the dose of PRRSV-infected blood that an individual mosquito must consume for transmission to occur. The reason transmission did not occur in replicates 1 and 2, despite detection of the virus in the mosquito homogenates by swine bioassay, is unknown. Possible explanations are that a sufficient quantity of the virus was not present within the mosquitoes that contacted the recipient pigs. The methods used to detect PRRSV in this study were not quantitative; therefore, again, no conclusion can be made regarding the amount of virus that the mosquitoes must carry to infect na?ve pigs.
        The source of the mosquitoes used for this study was a commercial swine farm. We preferred to use wild mosquitoes collected from a commercial swine setting, rather than laboratory-colonized mosquitoes, to better represent species of mosquitoes found under field conditions. In fact, A. vexans is the most commonly seen mosquito in the midwestern United States (Moon, unpublished data). However, the use of wild mosquitoes had certain limitations, including the inability to standardize the age of the mosquitoes. The source farm was PRRSV positive. We selected it because of convenient access, the fact that we had been observing tremendous numbers of mosquitoes both inside and outside the facility, and the fact that the facility was mechanically ventilated. Additionally, the virus isolated from the farm had been found to be 100% homologous (in the ORF 5 region of its nucleic acid) with the virus used for this study. Our selection of the source farm might be considered a limitation, since the virus isolated from the infected recipient pigs could have originated from the farm. However, clinical signs, such as coughing, fever, and depression, were not observed in any pigs housed on the farm, and viremia was not detected by PCR or VI in any pigs tested during the collection of mosquitoes. Furthermore, prior to initiation of each replicate, the collected mosquitoes were housed in the laboratory for 3 d, and representative samples (50 insects per replicate) were found to be negative by PCR, VI, and swine bioassay. Therefore, we feel that it is very unlikely that the mosquitoes contained PRRSV when collected.
        An important consideration when designing this study was the welfare of the pigs while they were in contact with the mosquitoes. During this period, the pigs were consciously restrained for approximately 2 h in Panepinto slings, which were specifically designed for surgical procedures. The pigs were upright, and the leg openings in the slings were lined with soft cloth to avoid irritation. A small fan propelled fresh air onto the pig's face, and the animals were frequently removed for exercise throughout the mosquito-contact period. They were given treats and fruit juice, and they appeared to be very comfortable in the slings, several falling asleep during the procedure. Finally, despite the numerous mosquito bites on the dorsal surface of the pigs, evidence of pruritus or secondary staphylococcal infection was not observed.
        In conclusion, our results indicate that PRRSV can be mechanically transmitted from infected to na?ve pigs by mosquitoes (A. vexans). This study did not address the question whether mosquitoes could also serve as biologic vectors of PRRSV. This virus is a member of the order Nidovirales, family Arteriviridae, genus Arterivirus (21). Although it is unlikely that Arterivirus members can replicate within insects (22), further studies are needed to determine whether PRRSV has a potential to replicate within A. vexans or any other mosquitoes and to evaluate the role of mosquitoes in the transmission of PRRSV throughout commercial swine-producing areas.

        </td></tr><tr valign="top"><td width="145">
        </td><td>Footnotes
        Acknowledgments
        We thank the National Pork Board, the Minnesota Pork Producers Association, and Genetiporc for financially supporting the project.
        Correspondence and reprint requests to Dr. Scott A. Dee, tel: 612-625-8781, fax: 612-625-1210, e-mail: deexx004@tc.umn.edu
        Received December 20, 2001. Accepted March 25, 2002.


        </td></tr><tr valign="top"><td width="145">Top
        Abstract
        Introduction
        Materials and methods
        Results
        Discussion
        References

        </td><td>References
        1.
        Dee SA, Joo HS, Polson DD, Marsh WE. Evaluation of the effects of nursery depopulation on the profitability of 34 pig farms. Vet Rec 1997;140:498?500. [PubMed].

        2.
        Dee SA, Joo HS, Pijoan C. Controlling the spread of PRRS virus in the breeding herd through management of the gilt pool. Swine Health Prod 1994;3:64?69.

        3.
        Christopher-Hennings J, Nelson EA, Hines RJ, et al. Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of adult boars. J Vet Diagn Invest 1995;7: 456?464. [PubMed].

        4.
        Otake S, Dee SA, Rossow KD, Joo HS, Deen J, Molitor TW. Transmission of porcine reproductive and respiratory syndrome virus by needles. Vet Rec 2002;150:114?115. [PubMed].

        5.
        Otake S, Dee SA, Rossow KD, Joo HS, Deen J, Molitor TW. Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls). Swine Health Prod 2002;10:59?65.

        6.
        Tidwell MA, Dean WD, Combs GP, Anderson DW, Cowart WO, Axtell RC. Transmission of hog cholera virus by horseflies (Tabanidae: Diptera) Am J Vet Res 1972;33:615?622.

        7.
        Stewart WC, Carbrey EA, Jenney EW, Kresse JI, Snyder ML, Wessman SJ. Transmission of hog cholera virus by mosquitoes. Am J Vet Res 1972;36:611?614.

        8.
        Gough PM, Jorgenson RD. Identification of porcine transmissible gastroenteritis virus in house flies (Musca domestica Linneaus). Am J Vet Res 1983;44:2078?2082. [PubMed].

        9.
        Medvecky I, Kovacs L, Kovacs F, Papp L. The role of the housefly, Musca domestica, in the spread of Aujeszky's disease (pseudorabies). Med Vet Entomol 1988;2:81?86. [PubMed].

        10.
        Yoon IJ, Joo HS, Christianson WT, Morrison RB, Dial GD. Persistent and contact infection in nursery pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Swine Health Prod 1993;1:5?8.

        11.
        Otake S, Dee SA, Rossow KD, Moon RD, Pijoan C. Transmission of porcine reproductive and respiratory syndrome virus by mosquitoes (Culicidae). Proc 82nd Conf Res Workers Anim Dis 2001:167.

        12.
        Bierk MD, Dee SA, Rossow KD, et al. Diagnostic investigation of chronic porcine reproductive and respiratory syndrome virus in a breeding herd of pigs. Vet Rec 2001;148:687?690. [PubMed].

        13.
        Newhouse VF, Chamberlain RW, Johnson JG, Sudia WD. Use of dry ice to increase mosquito catches of the CDC miniature light trap. Mosquito News 1966;26:30?35.

        14.
        Weber AF, Moon RD, Sorensen DK, et al. Evaluation of the stable fly (Stomoxys calcitrans) as a vector of enzootic bovine leukosis. Am J Vet Res 1988;49:1543?1549. [PubMed].

        15.
        Molitor TW, Tune KA, Shin J, Collins J, Kapur V. Applications of TaqManTM PCR in the detection of porcine reproductive and respiratory syndrome virus. Proc Allen D. Leman Conf 1997:173?175.

        16.
        Bautista EM, Goyal S, Yoon IJ, Joo HS, Collins JE. Comparison of porcine alveolar macrophages and CL 2621 for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and anti-PRRSV antibodies. J Vet Diagn Invest 1993;5:163?165. [PubMed].

        17.
        Murtaugh MP, Elam MR, Kakach LT. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch Virol 1995;140:1451?1460. [PubMed].

        18.
        Snyder ML, Mermer B, Anderson PR, Wensvoort G, Hill HT. Evaluation data for an immunological ELISA for PRRS. Proc 2nd Int Symp PRRSV 1995:15.

        19.
        Clements AN. The Biology of Mosquitoes. Vol. 2: Sensory Reception and Behavior. New York; CABI Publishing, 1999: 319?332.

        20.
        Sparagano OAE, Allsopp MTEP, Mank RA, Rijpkema JV, Figueroa JV, Jongejan F. Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review. Exp Appl Acarol 1999;23: 929?960. [PubMed] [Full Text].

        21.
        Cavanagh D. Nidovirales: a new order comprising Coronoviridae and Arteriviridae. Arch Virol 1997;142:629?633. [PubMed] [Full Text].

        22.
        Buriki F, Aspock H, Kunz C. Failure to propagate equine arteritis virus in an Aedine and an Anopheline mosquito species. Zentralbl Bakteriol Parasitenkd Infekt Hyg Erste Abtg 1972;219a: 109?111.



        </td></tr><tr valign="top"><td width="145">
        </td><td>Figures and Tables
        <table style="clear: both;" border="0" cellpadding="5" cellspacing="5"><tbody><tr align="left" valign="top"><td width="100">http://www.pubmedcentral.nih.gov/pic...bname=8FF1.gif</td><td>Figure 1. Method of mosquito containment during the manual vector-transmission protocol: a plastic transfer vial containing an individual mosquito is fastened with masking tape over the dorsal surface of the pig; the mosquito has access to the pig's skin (more ...)
        </td></tr></tbody></table><table style="clear: both;" border="0" cellpadding="5" cellspacing="5"><tbody><tr align="left" valign="top"><td width="100"></td><td>Table I.
        </td></tr></tbody></table>
        http://www.pubmedcentral.nih.gov/art...i?artid=227003
        </td></tr></tbody></table>
        </td></tr></tbody></table>

        Comment


        • #5
          Re: H5N1 in flies in Japan

          Hey! Thank you Snowy

          I work everyday with the swine PRRSvirus and had read alot about this but never found that article, I'm surprised and glad, I'll show this to my co-lab-mates when I'll come back next week.

          Comment


          • #6
            Re: H5N1 in flies in Japan

            We've known (see poultry biosecurity info) that insects can be mechanical vectors, i.e., virus hitches a ride on their feet, etc., but this internal habitation is a new tweek.

            Last fall/winter, I had read about how arthropods preceeded animals on the viral evolution timeline, evolving an effective accomodation. What I wondered then, was whether they could somehow "process/mutate" the virus, while not being infected?

            .
            "The next major advancement in the health of American people will be determined by what the individual is willing to do for himself"-- John Knowles, Former President of the Rockefeller Foundation

            Comment


            • #7
              Re: H5N1 in flies in Japan

              these flies, when they eat on something in the kitchen,
              they pump their saliva through their sucking tube onto the food
              and then suck in the solution. If the virus is in the saliva,
              that looks to me as if some viruses could remain on the food
              when the fly is disturbed and can't suck in all the solution.


              Has someone read the whole paper ?
              I'm interested in expert panflu damage estimates
              my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

              Comment


              • #8
                Re: H5N1 in flies in Japan

                I really appreciate the knowledge level of people on this forum.
                Thanks to all of you for sharing so much information. You've answered the questions I had on the subject.

                I had no idea that tests like that had been performed.
                This is my favorite place for information, even if I don't understand half of it.
                "There's a chance peace will come in your life - please buy one" - Melanie Safka
                "The greatest way to live with honor in this world is to be what we pretend to be" - Socrates

                Comment


                • #9
                  Re: H5N1 in flies in Japan

                  Originally posted by prepdeb
                  I really appreciate the knowledge level of people on this forum.
                  Thanks to all of you for sharing so much information. You've answered the questions I had on the subject.

                  I had no idea that tests like that had been performed.
                  This is my favorite place for information, even if I don't understand half of it.
                  We are all learning. Thanks for participating!

                  Comment

                  Working...
                  X