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PLoS ONE. Methamphetamine Reduces Human Influenza A Virus Replication

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  • PLoS ONE. Methamphetamine Reduces Human Influenza A Virus Replication

    [Source: PLoS ONE, full text: (LINK). Abstract, edited.]
    Methamphetamine Reduces Human Influenza A Virus Replication


    Yun-Hsiang Chen<SUP>1</SUP>, Kuang-Lun Wu<SUP>1</SUP>, Chia-Hsiang Chen<SUP>1</SUP><SUP>,</SUP><SUP>2</SUP><SUP>*</SUP>
    <SUP></SUP>
    1 Division of Mental Health and Addiction Medicine, Institute of Population Health Sciences, National Health Research Institutes, Zhunan, Taiwan, 2 Department of Psychiatry, Chang Gung Memorial Hospital at Linkou and Chang Gung University School of Medicine, Taoyuan, Taiwan



    Abstract

    Methamphetamine (meth) is a highly addictive psychostimulant that is among the most widely abused illicit drugs, with an estimated over 35 million users in the world. Several lines of evidence suggest that chronic meth abuse is a major factor for increased risk of infections with human immunodeficiency virus and possibly other pathogens, due to its immunosuppressive property. Influenza A virus infections frequently cause epidemics and pandemics of respiratory diseases among human populations. However, little is known about whether meth has the ability to enhance influenza A virus replication, thus increasing severity of influenza illness in meth abusers. Herein, we investigated the effects of meth on influenza A virus replication in human lung epithelial A549 cells. The cells were exposed to meth and infected with human influenza A/WSN/33 (H1N1) virus. The viral progenies were titrated by plaque assays, and the expression of viral proteins and cellular proteins involved in interferon responses was examined by Western blotting and immunofluorescence staining. We report the first evidence that meth significantly reduces, rather than increases, virus propagation and the susceptibility to influenza infection in the human lung epithelial cell line, consistent with a decrease in viral protein synthesis. These effects were apparently not caused by meth?s effects on enhancing virus-induced interferon responses in the host cells, reducing viral biological activities, or reducing cell viability. Our results suggest that meth might not be a great risk factor for influenza A virus infection among meth abusers. Although the underlying mechanism responsible for the action of meth on attenuating virus replication requires further investigation, these findings prompt the study to examine whether other structurally similar compounds could be used as anti-influenza agents.



    Citation: Chen Y-H, Wu K-L, Chen C-H (2012) Methamphetamine Reduces Human Influenza A Virus Replication. PLoS ONE 7(11): e48335. doi:10.1371/journal.pone.0048335

    Editor: Michael Nevels, University of Regensburg, Germany

    Received: June 20, 2012; Accepted: September 24, 2012; Published: November 6, 2012

    Copyright: ? 2012 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Funding: Source of funding: National Health Research Institutes. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

    Competing interests: The authors have declared that no competing interests exist.

    * E-mail: cchen@nhri.org.tw
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