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Macromol Rapid Commun. 2013 Feb 6. doi: 10.1002/marc.201200819. [Epub ahead of print]
Polydiacetylene Liposome Microarray Toward Influenza A Virus Detection: Effect of Target Size on Turn-On Signaling.
Seo S, Lee J, Choi EJ, Kim EJ, Song JY, Kim J.
Source
Macromolecular Science and Engineering, University of Michigan, 2300 Hayward St., Ann Arbor, MI 48109-2136, USA.
Abstract
Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe-target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe-target complexes not the strength of the probe-target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.
Copyright ? 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
23386374
[PubMed - as supplied by publisher]
Polydiacetylene Liposome Microarray Toward Influenza A Virus Detection: Effect of Target Size on Turn-On Signaling.
Seo S, Lee J, Choi EJ, Kim EJ, Song JY, Kim J.
Source
Macromolecular Science and Engineering, University of Michigan, 2300 Hayward St., Ann Arbor, MI 48109-2136, USA.
Abstract
Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe-target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe-target complexes not the strength of the probe-target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.
Copyright ? 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
23386374
[PubMed - as supplied by publisher]
