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Nanomedicine. 2013 Mar 7. pii: S1549-9634(13)00075-0. doi: 10.1016/j.nano.2013.02.009. [Epub ahead of print]
Induction of innate immunity in lungs with virus-like nanoparticles leads to protection against influenza and Streptococcus pneumoniae challenge.
Mathieu C, Rioux G, Dumas MC, Leclerc D.
Source
Department of Microbiology, Infectiology and Immunology, Infectious Disease Research Centre/CHU de Qu?bec, Laval University, 2705 boul. Laurier, Quebec city, PQ, Canada G1V 4G2.
Abstract
Nanoparticles comprised of the coat protein of a plant virus (papaya mosaic virus; PapMV) and a single-stranded RNA (ssRNA), triggers a strong innate immune stimulation in the lungs of the animals a few hours following instillation. A rapid recruitment of neutrophils, monocytes/macrophages and lymphocytes follows. This treatment was able to provide protection to an influenza challenge that lasts at least 5 days. Protection could be recalled for longer periods by repeating the instillations once per week for more than 10 weeks. The treatment also conferred protection to a lethal challenge with Streptococcus pneumoniae-the major cause of bacterial pneumonia. Finally, we also showed that the nanoparticles could be used to treat mice infected with influenza and significantly decrease morbidity. These data strengthen the potential for using PapMV nanoparticles as a non-specific inducers of the innate immune response in lungs during viral pandemics or to combat bioterrorist attack.
Copyright ? 2013. Published by Elsevier Inc.
PMID:
23499666
[PubMed - as supplied by publisher]
Induction of innate immunity in lungs with virus-like nanoparticles leads to protection against influenza and Streptococcus pneumoniae challenge.
Mathieu C, Rioux G, Dumas MC, Leclerc D.
Source
Department of Microbiology, Infectiology and Immunology, Infectious Disease Research Centre/CHU de Qu?bec, Laval University, 2705 boul. Laurier, Quebec city, PQ, Canada G1V 4G2.
Abstract
Nanoparticles comprised of the coat protein of a plant virus (papaya mosaic virus; PapMV) and a single-stranded RNA (ssRNA), triggers a strong innate immune stimulation in the lungs of the animals a few hours following instillation. A rapid recruitment of neutrophils, monocytes/macrophages and lymphocytes follows. This treatment was able to provide protection to an influenza challenge that lasts at least 5 days. Protection could be recalled for longer periods by repeating the instillations once per week for more than 10 weeks. The treatment also conferred protection to a lethal challenge with Streptococcus pneumoniae-the major cause of bacterial pneumonia. Finally, we also showed that the nanoparticles could be used to treat mice infected with influenza and significantly decrease morbidity. These data strengthen the potential for using PapMV nanoparticles as a non-specific inducers of the innate immune response in lungs during viral pandemics or to combat bioterrorist attack.
Copyright ? 2013. Published by Elsevier Inc.
PMID:
23499666
[PubMed - as supplied by publisher]
