3 D PB2

[Anne]

a good and " funny"

site with 3 D , but to difficult for me

click here :

http://molvis.sdsc.edu/fgij/fg.htm?mol=2JDQ

Article

<hr class="separator"> Nature Structural & Molecular Biology 14, 229 - 233 (2007)
Published online: 25 February 2007 | <abbr title="Digital Object Identifier">doi</abbr>:10.1038/nsmb1212
Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit

Franck Tarendeau, Julien Boudet, Delphine Guilligay, Philippe J Mas, Catherine M Bougault, S&#233;bastien Boulo, Florence Baudin, Rob W H Ruigrok, Nathalie Daigle, Jan Ellenberg, Stephen Cusack, Jean-Pierre Simorre & Darren J Hart
The structure(s) can be viewed in 3D using a simple, freely available molecular visualization tool, FirstGlance in Jmol

• 2JDQ

[gsgs]

Re: 3 D PB2

is it 2 PB2s ? The protein, which together with PB1,PA and lots
of NPs for the 8 nucleocapsids which surround and protect
the RNA-segments

[Anne]

Re: 3 D PB2

I don't know.. I have the same question it's the first time that I see a PB2.
it s very symetric.

[gsgs]

Re: 3 D PB2

article here:

http://www.nature.com/nsmb/journal/v14/n3/full/nsmb1212.html

I remember, we had pictures of HA here before.
I search a link...


2 strains with identical amino acids (click on the atoms)
I can see amino-acids 84-504 and then 686-734 with gaps

[Mamabird]

Re: 3 D PB2

The two larger units represent the importin subunits, while the two smaller units represent an 80 amino section of PB2. So, you are only seeing a very small piece of this very large Influenza A protein.

[gsgs]

Re: 3 D PB2

well, more than the half - just counting the amino-acids.

where do they say, it's PB2 ? PB2 is a monomer, not dimer


Can we have the same for HA, please ?
With the umbrellas and/or cones, cleavage site,
demonstration of some vaccine-escaping mutations

[Anne]

Re: 3 D PB2

thank you mama bird..
and thank you gsgs .
I must read this article.. I imagined it was on restricted acces ( don't know why )
perhaps PB2 ( B for basic ) 2 : because it was find after PB1..

I was amused by this site. I think it could be usefull ( not for me ).

[Mamabird]

Re: 3 D PB2

well, more than the half - just counting the amino-acids.

where do they say, it's PB2 ? PB2 is a monomer, not dimer

If you mouse click over the 3-D image, you will see that the two smaller units represent the C Terminus of the Polymerase Basic Subunit 2 (chain D and E) which is amino acid positions 678-759 (end of the protein chain).

The two larger units (chain A and B) represent the Importin subunits (alpha and beta) that serve as nuclear transport mechanisms to get the very large PB2 protein through the pores of the host cells nuclear membrane. Then replication can take place with a vengance.

It is a bit confusing to call this protein PB2, as Influenza proteins are generally ranked according to size. PB2 is first and is 759 amino acids long while the second place protein, PB1 is 757 amino acids long.

[gsgs]

Re: 3 D PB2

ahh, the "Importin" is part of the host, not of the virus.

[Anne]

Re: 3 D PB2

another site : http://www.umass.edu/microbio/chime/...tlas/atlas.htm

wich lead to http://www.pdb.org/pdb/static.do?p=e...nth/index.html

and to

Importin
http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html


inside your cells, the process of protein synthesis is separated into two compartments. The first half of the job, when DNA is transcribed into RNA, is performed in the nucleus. The second half is then performed outside the nucleus, when ribosomes translate the RNA to construct proteins in the cytoplasm.
This separation requires a continuous traffic of molecules: new RNA molecules must be transported out of the nucleus and nuclear proteins, such as newly-synthesized histones or polymerases, must be transported back into the nucleus. Huge tube-shaped nuclear pores act as the highway connecting the nucleus and the cytoplasm, and importins and exportins (collectively known as karyopherins) ferry molecules back and forth through the pore

importin alpha : 1EE5

http://molvis.sdsc.edu/fgij/fg.htm?mol=1EE5

[Anne]

Re: 3 D PB2

http://www.pdb.org/pdb/results/results.do

[Anne]

Re: 3 D PB2

http://www.pasteur.fr/recherche/unites/REG/Presse.shtml#Hot

Pasteur institute.

MACHINE TRANSLATION

March 20, 2008. An international symposium joining together the Fudan University of Shanghai, the CNCBD and the EFBIC drew up a general panorama of the situation of the infectious illness in the world, by stressing the co-operation between Europe and China in an important combat for very whole humanity. One of the principal differences between the form of the highly pathogenic flu virus at the similar birds and forms in the mammals comes from the stage of multiplication of the viral genome in the core of cells of the host, although it is about a virus with ARN. It is what explains why the changes which could be at the origin of a pandemia will probably blame the r?plicase virus in its mode of interaction with protein of the host, the importine, which allows the r?plicase unit/viral genome to enter the cellular core. It is this system which allows the fast replication of the virus, and this stage is thus crucial to decide number of the viral particles which can be synthesized in a given cell.

[gsgs]

Re: 3 D PB2

> the number of the viral particles which can be synthesized in a given cell.

I had read it's 100000-1000000 per cell within 6-10 hours.
So after some replication cycles there must be lots
of viruses searching for free cells in the neighborhood.
They are competing for the cells.
What happens with the zillions of viruses
who can't find a free cell ?

[Anne]

Re: 3 D PB2

shedding ? surviving in blood ? ( a " long " viremy is observed with H5N1 )