H5N1 Vietnam 2008 Clade 7

[HenryN]

LOCUS FJ842481 1713 bp cRNA linear VRL 27-MAR-2009
DEFINITION Influenza A virus (A/chicken/Vietnam/NCVD-03/2008(H5N1)) segment 4
hemagglutinin (HA) gene, complete cds.
ACCESSION FJ842481
VERSION FJ842481.1 GI:225697337
KEYWORDS .
SOURCE Influenza A virus (A/chicken/Vietnam/NCVD-03/2008(H5N1))
ORGANISM Influenza A virus (A/chicken/Vietnam/NCVD-03/2008(H5N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1713)
AUTHORS Nguyen,T., Davis,T., Stembridge,W., Shu,B., Balish,A., Inui,K.,
Do,H., Ngo,H., Wan,X.-F., McCarron,M., Lindstrom,S., Cox,N.,
Nguyen,C., Klimov,A. and Donis,R.
TITLE Characterization of a highly pathogenic avian influenza H5N1 virus
sublineage in poultry seized at ports of entry into Vietnam
JOURNAL Virology (2009) In press
REFERENCE 2 (bases 1 to 1713)
AUTHORS Nguyen,T., Davis,T., Stembridge,W., Shu,B., Balish,A., Inui,K.,
Do,H., Ngo,H., Wan,X.-F., McCarron,M., Lindstrom,S., Cox,N.,
Nguyen,C., Klimov,A. and Donis,R.
TITLE Direct Submission
JOURNAL Submitted (19-MAR-2009) Influenza Division, CDC, 1600 Clifton Rd.,
Atlanta, GA 30333, USA
FEATURES Location/Qualifiers
source 1..1713
/organism="Influenza A virus
(A/chicken/Vietnam/NCVD-03/2008(H5N1))"
/mol_type="viral cRNA"
/strain="A/chicken/Vietnam/NCVD-03/2008"
/serotype="H5N1"
/isolation_source="cloacal swab"
/host="chicken"
/db_xref="taxon:629212"
/segment="4"
/country="Viet Nam"
/collection_date="2008"
gene 1..1713
/gene="HA"
CDS 1..1713
/gene="HA"
/codon_start=1
/product="hemagglutinin"
/protein_id="ACO07038.1"
/db_xref="GI:225697338"
/translation="MEKIVLLLAIISLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH
AQDILEKTHNGKLCNLDGVKPLILKDCSVAGWLLGNPMCDEFLNVSEWSY IVEKASPA
NGLCYPGDFNDYEELKHLLNRITHLKKIKIIPKSYWSNHEASSGVSAACS YMGEPSFF
RNVVWLIKKNNTYPPIKVNYTNTNQEDLLVLWGIHHPNNEAEQIQIYQNL ITHISVGT
STLNQRLIPKIATRSKVNGQSGRMEFFWTILKPNDSINFDSNGNFIAPEY AYIVKKGD
SAIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRL VLATGLRN
APQREREGGRRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAA DKESTQKA
IDGITNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTY NAELLVLM
ENERTLDFHDSNVKNLYEKVRLQLRDNAKELGNGCFEFYHKCDNECMESV RNGTYDYS
QYSEEARLSREEINGVKLESMVTYQILSIYSTVASSLALAIMVAGLSLWM CSNGSLQC
RICI"
ORIGIN
1 atggagaaaa tagtgcttct tcttgcaata atcagccttg ttaaaagtga tcagatttgc
61 attggttacc atgcaaacaa ctcgacagag caggttgaca caataatgga aaagaacgtt
121 actgttacac atgctcaaga catactggag aagacacaca acgggaagct ctgcaaccta
181 gatggagtga agcctctaat tctaaaagac tgtagtgtag ctggatggct cctcgggaac
241 ccaatgtgtg acgaatttct caatgtgtca gaatggtctt acatagtgga gaaggccagt
301 ccagccaatg gcctctgtta cccaggggat ttcaatgact atgaagaact gaaacaccta
361 ttaaacagaa taacacatct taagaaaatt aagatcatcc ccaaaagtta ttggtccaat
421 catgaagcct catcaggggt gagcgcagca tgttcctata tgggggagcc ctcctttttc
481 agaaatgtgg tatggcttat caaaaagaat aatacatacc caccaataaa ggtgaactac
541 accaatacca atcaagaaga tcttttggta ctgtggggga ttcaccatcc caataatgag
601 gcagagcaga tacagatcta tcaaaaccta atcacccata tttcagttgg aacatcaaca
661 ctaaaccaga gattgatacc aaaaatagct actagatcca aagtgaacgg gcaaagtgga
721 agaatggagt tcttctggac aattttaaag ccgaatgatt ctatcaattt cgatagcaat
781 ggaaatttca ttgctccaga atatgcctac attgtcaaga aaggggactc ggcaattatg
841 aaaagtgaat tggagtatgg taactgcaac accaagtgtc aaactccaat gggggcgata
901 aattctagta tgccattcca caacatacac cctctcacca tcggggaatg ccccaaatat
961 gtgaagtcaa acagattagt cctcgcgact ggactcagaa atgcccctca aagagaaaga
1021 gaggggggaa gaagaagaaa aagaggacta tttggagcca tagcagggtt tatagaggga
1081 ggatggcagg gaatggtaga tggttggtat gggtaccacc atagcaatga gcaggggagt
1141 gggtacgctg cagacaaaga atccactcaa aaggcaatag atggaatcac taataaggtc
1201 aactcgatca ttgacaaaat gaacactcaa tttgaggccg ttggaaggga atttaataac
1261 ttagagagga gaatagaaaa tttaaacaaa aagatggagg acggattcct agatgtctgg
1321 acttataacg ctgaacttct ggttctcatg gaaaatgaga gaactctaga ctttcatgac
1381 tcaaatgtca agaaccttta cgaaaaggtc cgactacagc ttagggataa tgcaaaggag
1441 ctgggtaacg gttgtttcga gttctaccac aaatgtgata atgaatgtat ggaaagtgta
1501 agaaacggaa cgtatgacta ctcgcagtat tcagaagaag caagactaag cagagaggaa
1561 ataaatggag taaaattgga atcaatggta acttaccaaa tactgtcaat ttattcaaca
1621 gtggcgagtt ccctagcatt ggcaatcatg gtggctggtc tatctttatg gatgtgctcc
1681 aatggatcgt tacaatgcag aatttgcatt tga

[HenryN]

Sequence has a 3 BP deletion (downstream from Shanxi/2 deletion) as well as 9 BP insertion at HA cleavages site, reflection clade 7 instability in Northern Vietnam ex-China.

[HenryN]

Originally Posted by niman

Sequence has a 3 BP deletion (downstream from Shanxi/2 deletion) as well as 9 BP insertion at HA cleavages site, reflection clade 7 instability in Northern Vietnam ex-China.

Earlier clade 7 sequence from Vietnam has a 3 BP insertion to create novel cleavage site REGGRRRKR (the above sequence matches but has a 6 BP duplication to create REREGGRRRKR).

It is VERY likely that both of these sequences originated in China.

[HenryN]

Note title:

TITLE Characterization of a highly pathogenic avian influenza H5N1 virus
sublineage in poultry seized at ports of entry into Vietnam

[HenryN]

Commentary at

http://www.recombinomics.com/News/03..._Unstable.html

[AlaskaDenise]

Originally Posted by niman

Commentary at

http://www.recombinomics.com/News/03..._Unstable.html

Commentary

Unstable Clade 7 H5N1 In China Raises Concerns

Recombinomics Commentary 10:34
March 30, 2009

A recent clade 7 HA sequence from a chicken in Vietnam, A/chicken/Vietnam/NCVD-03/2008 was released at Genbank under the title, "Characterization of a highly pathogenic avian influenza H5N1 sublineage in poultry seized at ports of entry into Vietnam". It was proposed as a vaccine target in the recent WHO update because it was antigenically distinct from another recent clade 7 target, A/chicken/Vietnam/NCVD-016/2008, which was isolate in northern Vietnam near the border with China. Both of these sequences have a cleavage site similar to clade 7 isolated in 2006 in China, except the cleavage site has a 3 BP insertion, encoding for an additional basic amino acid.

However, the recent sequence also has a duplication of sequences encoding the two amino acids at the beginning of the cleavage site, creating the novel cleavage site, REREGGRRRKR. The sequence also has an addition 3 BP deletion, which is distinct from the 3 BP deletion found in earlier clade 7 isolates from China, as well as clade 2.2 isolates in Egypt.

The selection of two 2008 clade 7 targets from Vietnam is unusual, but follows a spate of cases in China in January. These cases followed confirmed clade 7 outbreaks in poultry in Jiangsu in December, 2008 and strongly suggest that most of the human cases in China were clade 7.

The sequences form these patients have not been released and have only been described in general terms in reports from China. The WHO report was silent on all cases from China except for two that were the Fujian strain (clade 2.3.2 and 2.3.4), further supporting concerns that vaccine resistant clade 7 is widely circulating in China.

The recent sequences from northern Vietnam demonstrate considerable genetic instability that involves a large number of non-synonymous changes as well as insertions and deletions in the HA sequence.

Release of sequences from the Jiangsu poultry outbreaks in China, as well as recent human cases in China and northern Vietnam would be useful.

.

[AlaskaDenise]

REREGGRRRKR

These are all cleavage site positions?

If so, that's more than in the past, so is H5N1 adapting to be cleaved my more enzymes with one strain?

.

[HenryN]

Originally Posted by AlaskaDenise

These are all cleavage site positions?

If so, that's more than in the past, so is H5N1 adapting to be cleaved my more enzymes with one strain?

.

Cleavage is after an R or K.

[AlaskaDenise]

Originally Posted by niman

Cleavage is after an R or K.

???

Could you provide more detail?

.

[HenryN]

Commentary

http://www.recombinomics.com/News/03..._Cleavage.html

[HenryN]

1: FJ538950	Reports	Links
Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1)) segment 7 matrix protein 2 (M2) and matrix protein 1 (M1) genes, complete cds gi|218137997|gb|FJ538950.1|[218137997]

2: FJ538949	Reports	Links
Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1)) segment 6 neuraminidase (NA) gene, partial cds gi|218137995|gb|FJ538949.1|[218137995]

3: FJ538948	Reports	Links
Influenza A virus (A/duck/Vietnam/NCVD-100/2007(H5N1)) segment 7 matrix protein 2 (M2) and matrix protein 1 (M1) genes, complete cds gi|218137992|gb|FJ538948.1|[218137992]

4: FJ538947	Reports	Links
Influenza A virus (A/duck/Vietnam/NCVD-100/2007(H5N1)) segment 6 neuraminidase (NA) gene, partial cds gi|218137990|gb|FJ538947.1|[218137990]

[HenryN]

LOCUS FJ538949 1349 bp cRNA linear VRL 04-APR-2009
DEFINITION Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1)) segment 6
neuraminidase (NA) gene, partial cds.
ACCESSION FJ538949
VERSION FJ538949.1 GI:218137995
KEYWORDS .
SOURCE Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1))
ORGANISM Influenza A virus (A/chicken/Vietnam/NCVD-093/2008(H5N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1349)
AUTHORS Deyde,V.M., Nguyen,T., Bright,R.A., Balish,A., Shu,B.,
Lindstrom,S., Klimov,A.I. and Gubareva,L.V.
TITLE Detection of molecular markers of antiviral resistance in influenza
A (H5N1) viruses using a pyrosequencing method
JOURNAL Antimicrob. Agents Chemother. 53 (3), 1039-1047 (2009)
PUBMED 19124660
REFERENCE 2 (bases 1 to 1349)
AUTHORS Davis,T. and Nguyen,T.
TITLE Direct Submission
JOURNAL Submitted (09-DEC-2008) MVVB, CDC, 1600 Clifton Rd., Atlanta, GA
30333, USA
FEATURES Location/Qualifiers
source 1..1349
/organism="Influenza A virus
(A/chicken/Vietnam/NCVD-093/2008(H5N1))"
/mol_type="viral cRNA"
/strain="A/chicken/Vietnam/NCVD-093/2008"
/serotype="H5N1"
/host="chicken"
/db_xref="taxon:581024"
/segment="6"
/country="Viet Nam"
/collection_date="2008"
gene 1..>1349
/gene="NA"
CDS 1..>1349
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACK57573.1"
/db_xref="GI:218137996"
/translation="MNPNQKIITIGSICVVIGIVSLMLQIGNMISIWVSHSIQTENQC
QAESISNTKFLTEKAVASVTLMGNSSLCPISGWAVHSKDNSIRIGSKGDV FVIREPFI
SCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNS RFESVAWS
ASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESE CACVNGSC
FTVMTDGPSNGQASYKIFKLEKGKVVKSVELDAPNYHYEECSCYPDAGEI TCVCRDNW
HGSNGPWVSFNHYLEYQIGYICSGVFGDNPRPNDGTGSCGPVSPNGAYGV KGFSFKYG
NGVWIGRTKSTNSRSGFEMIWDPNGWTGTDSSFSVKQDIVAITDWSGYSG SFVQHPEL
TGLDCIRPCFWVELVRGRPKESTIWTSGSSISFCGVNSDTVGWSWPDGAE VPFTIDK"
ORIGIN
1 atgaatccaa atcagaagat aataaccatc ggatcaatct gtgtggtaat tggaatagtt
61 agcttaatgt tacaaattgg gaacatgatc tcaatatggg tcagtcattc aattcagaca
121 gagaatcaat gccaagctga atcgatcagc aatactaaat ttctcactga gaaggctgtg
181 gcttcagtaa cattaatggg caattcatct ctttgcccca ttagcggatg ggctgtacac
241 agtaaggaca acagcataag gatcggttcc aagggggatg tgtttgttat aagagagccg
301 ttcatctcat gctcccactt ggaatgcaga actttctttt tgactcaggg agccttgctg
361 aatgacaagc actccaatgg gactgtcaaa gacagaagcc ctcacagaac attaatgagt
421 tgtcctgtgg gtgaggctcc ctctccatat aactcaaggt ttgagtctgt tgcttggtca
481 gcaagtgctt gccatgatgg caccagttgg ttgacaattg gaatttctgg cccagacaat
541 ggggctgtgg ctgtattgaa atacaatggt ataataacag acactatcaa aagttggagg
601 aacaacatac tgagaactca agagtctgaa tgtgcatgtg taaatggctc ttgctttact
661 gtaatgactg atggaccaag taatgggcag gcatcatata agatcttcaa attggaaaaa
721 gggaaagtgg ttaaatcagt tgaattggat gctcctaatt atcactatga ggaatgctcc
781 tgttatcctg atgctggcga aatcacatgt gtgtgcaggg ataattggca tggctcaaat
841 gggccatggg tatctttcaa tcactatttg gagtatcaaa taggatatat atgcagtgga
901 gttttcggag acaatccacg ccccaatgat ggaacaggga gctgtggtcc ggtgtcccct
961 aatggggcat atggggtaaa agggttttca tttaaatacg gcaatggtgt ttggatcggg
1021 agaaccaaaa gcactaattc caggagcggc tttgaaatga tttgggatcc gaatgggtgg
1081 actggaacgg acagcagctt ttcggtgaag caagatatcg tagcaataac tgattggtca
1141 ggatatagcg ggagttttgt ccagcatcca gaactgacag gattagattg cataagacct
1201 tgtttctggg ttgagttagt cagagggcgg cccaaagaga gcacaatctg gactagtggg
1261 agcagcatat ccttttgtgg tgtaaatagt gacactgtgg gttggtcttg gccagacggt
1321 gctgaggtgc cattcaccat tgacaagta

[HenryN]

The above isolate (clade 7) is in the HA tree of WHO vaccine targets and the above NA sequence has OBVIOUS recombination (with vaccine resistant clade 2.2 in Egypt).

[HenryN]

NA travel log

gb|FJ538949.1| Influenza A virus (A/chicken/Vietnam/NCVD-093/... 38.2 0.17
gb|FJ719836.1| Influenza A virus (A/chicken/Manipur/NIV9743/2... 38.2 0.17
gb|FJ667187.1| Influenza A virus (A/chicken/Domodedovo/MK/200... 38.2 0.17
gb|FJ688369.1| Influenza A virus (A/chicken/Egypt/24NLQP-CLEV... 38.2 0.17
gb|FJ688367.1| Influenza A virus (A/chicken/Egypt/17NLQP-CLEV... 38.2 0.17
gb|FJ688366.1| Influenza A virus (A/chicken/Egypt/15NLQP-CLEV... 38.2 0.17
gb|FJ688365.1| Influenza A virus (A/chicken/Egypt/10NLQP-CLEV... 38.2 0.17
gb|FJ688364.1| Influenza A virus (A/chicken/Egypt/4NLQP-CLEVB... 38.2 0.17
gb|FJ688363.1| Influenza A virus (A/chicken/Egypt/1NLQP-CLEVB... 38.2 0.17
emb|AM911101.1| Influenza A virus (A/Anas acuta/Slovenia/470/... 38.2 0.17
emb|AM911091.1| Influenza A virus (A/Ardea cinerea/Slovenia/1... 38.2 0.17
emb|AM911086.1| Influenza A virus (A/cygnus olor/Slovenia/156... 38.2 0.17
emb|AM911074.1| Influenza A virus (A/Mergus albellus/Slovakia... 38.2 0.17
emb|AM911073.1| Influenza A virus (A/Peregrine falcon/Slovaki... 38.2 0.17
gb|EU636810.1| Influenza A virus (A/great crested grebe/Basqu... 38.2 0.17
gb|FJ461659.1| Influenza A virus (A/Egypt/3401-NAMRU3/2008(H5... 38.2 0.17
gb|FJ461658.1| Influenza A virus (A/Egypt/3300-NAMRU3/2008(H5... 38.2 0.17
gb|FJ461652.1| Influenza A virus (A/Egypt/10217-NAMRU3/2007(H... 38.2 0.17
gb|FJ461651.1| Influenza A virus (A/Egypt/10216-NAMRU3/2007(H... 38.2 0.17
gb|FJ461650.1| Influenza A virus (A/Egypt/10215-NAMRU3/2007(H... 38.2 0.17
gb|FJ461648.1| Influenza A virus (A/Egypt/6251-NAMRU3/2007(H5... 38.2 0.17
gb|FJ461644.1| Influenza A virus (A/Egypt/2751-NAMRU3/2007(H5... 38.2 0.17
gb|FJ461641.1| Influenza A virus (A/Egypt/2630-NAMRU3/2007(H5... 38.2 0.17
gb|FJ461632.1| Influenza A virus (A/Egypt/1731-NAMRU3/2007(H5... 38.2 0.17
gb|FJ461631.1| Influenza A virus (A/Egypt/1604-NAMRU3/2007(H5... 38.2 0.17
gb|FJ461629.1| Influenza A virus (A/Egypt/2991-NAMRU3/2006(H5... 38.2 0.17
gb|FJ461628.1| Influenza A virus (A/Egypt/2947-NAMRU3/2006(H5... 38.2 0.17
gb|EU889100.1| Influenza A virus (A/herring gull/Sweden/V1116... 38.2 0.17
gb|EU889099.1| Influenza A virus (A/tufted duck/Sweden/V599/2... 38.2 0.17
gb|EU748905.1| Influenza A virus (A/falcon/Saudi Arabia/D1795... 38.2 0.17
gb|EU717858.1| Influenza A virus (A/chicken/Egypt/1709-6/2008... 38.2 0.17
gb|EU717856.1| Influenza A virus (A/chicken/Egypt/1709-5/2008... 38.2 0.17
gb|EU717854.1| Influenza A virus (A/chicken/Egypt/1709-4VIR08... 38.2 0.17
gb|EU717852.1| Influenza A virus (A/duck/Egypt/1709-3VIR08/20... 38.2 0.17
gb|EU717850.1| Influenza A virus (A/chicken/Egypt/1709-1VIR08... 38.2 0.17
gb|EU697218.1| Influenza A virus (A/chicken/Nigeria/228-5/200... 38.2 0.17
gb|EU697225.1| Influenza A virus (A/chicken/Nigeria/228-6/200... 38.2 0.17
gb|EU697233.1| Influenza A virus (A/chicken/Nigeria/228-10/20... 38.2 0.17
gb|EU574921.1| Influenza A virus (A/chicken/Israel/1055/2008(... 38.2 0.17
gb|EU443567.1| Influenza A virus (A/Cygnus olor/Czech Republi... 38.2 0.17
gb|EU443566.1| Influenza A virus (A/Mergus albellus/Slovakia/... 38.2 0.17
gb|EU443565.1| Influenza A virus (A/Peregrine falcon/Slovakia... 38.2 0.17
gb|EU443564.1| Influenza A virus (A/Cygnus olor/Czech Republi... 38.2 0.17
emb|AM498620.1| Influenza A virus (A/mute swan/France/06254a/... 38.2 0.17
emb|AM498619.1| Influenza A virus (A/mute swan/France/06303/2... 38.2 0.17
emb|AM498617.1| Influenza A virus (A/common pochard/France/06... 38.2 0.17
gb|EU146881.1| Influenza A virus (A/chicken/Egypt/3/2006(H5N1... 38.2 0.17
gb|EU146882.1| Influenza A virus (A/Egypt/902782/2006(H5N1)) ... 38.2 0.17
gb|EU146883.1| Influenza A virus (A/Egypt/902786/2006(H5N1)) ... 38.2 0.17
gb|EU146891.1| Influenza A virus (A/chicken/Egypt/1/2006(H5N1... 38.2 0.17
gb|EU146892.1| Influenza A virus (A/chicken/Egypt/2/2006(H5N1... 38.2 0.17
gb|EF619973.1| Influenza A virus (A/turkey/Turkey/1/2005(H5N1... 38.2 0.17
emb|AM262563.1| Influenza A virus (A/chicken/Nigeria/SO494/20... 38.2 0.17
emb|AM262562.1| Influenza A virus (A/chicken/Nigeria/SO493/20... 38.2 0.17
emb|AM262561.1| Influenza A virus (A/chicken/Nigeria/SO452/20... 38.2 0.17
emb|AM262560.1| Influenza A virus (A/chicken/Nigeria/SO300/20... 38.2 0.17
gb|EF532643.1| Influenza A virus (A/duck/Gaza/760/2006(H5N1))... 38.2 0.17
gb|EF532642.1| Influenza A virus (A/chicken/Gaza/714/2006(H5N... 38.2 0.17
gb|EF532641.1| Influenza A virus (A/chicken/Gaza/713/2006(H5N... 38.2 0.17
gb|EF532640.1| Influenza A virus (A/chicken/Israel/625/2006(H... 38.2 0.17
gb|EF532639.1| Influenza A virus (A/chicken/Gaza/450/2006(H5N... 38.2 0.17
gb|EF532638.1| Influenza A virus (A/turkey/Israel/446/2006(H5... 38.2 0.17
gb|EF532637.1| Influenza A virus (A/chicken/Israel/397/2006(H... 38.2 0.17
gb|EF532636.1| Influenza A virus (A/turkey/Israel/365/2006(H5... 38.2 0.17
gb|EF532635.1| Influenza A virus (A/turkey/Israel/364/2006(H5... 38.2 0.17
gb|EF532634.1| Influenza A virus (A/turkey/Israel/345/2006(H5... 38.2 0.17
gb|EF532633.1| Influenza A virus (A/duck/Gaza/834/2006(H5N1))... 38.2 0.17
gb|EF486250.1| Influenza A virus (A/goose/Egypt/13009N3-SM2/2... 38.2 0.17
gb|EF486249.1| Influenza A virus (A/duck/Egypt/1888N3-SM25/20... 38.2 0.17
gb|EF486248.1| Influenza A virus (A/duck/Egypt/13010N3-CLEVB/... 38.2 0.17
gb|EF486247.1| Influenza A virus (A/duck/Egypt/12380N3-CLEVB/... 38.2 0.17
gb|EF486246.1| Influenza A virus (A/chicken/Egypt/1892N3-HK49... 38.2 0.17
gb|EF486245.1| Influenza A virus (A/chicken/Egypt/1891N3-CLEV... 38.2 0.17
gb|EF486244.1| Influenza A virus (A/chicken/Egypt/1890N3-HK45... 38.2 0.17
gb|EF486243.1| Influenza A virus (A/chicken/Egypt/1889N3-SM26... 38.2 0.17
gb|EF486242.1| Influenza A virus (A/chicken/Egypt/12379N3-CLE... 38.2 0.17
gb|EF486241.1| Influenza A virus (A/chicken/Egypt/12378N3-CLE... 38.2 0.17
gb|EF486240.1| Influenza A virus (A/chicken/Egypt/1129N3-HK9/... 38.2 0.17
gb|EF447431.1| Influenza A virus (A/chicken/Russia_Moscow obl... 38.2 0.17
gb|CY020655.1| Influenza A virus (A/turkey/Egypt/2253-2/2006(... 38.2 0.17
gb|CY020647.1| Influenza A virus (A/chicken/Egypt/2253-1/2006... 38.2 0.17
gb|CY020351.1| Influenza A virus (A/cygnus olor/Italy/808/200... 38.2 0.17
gb|EF474448.1| Influenza A virus (A/chicken/Moscow/2/2007(H5N... 38.2 0.17
gb|EF222324.1| Influenza A virus (A/Egypt/12374-NAMRU3/2006(H... 38.2 0.17
gb|EF222323.1| Influenza A virus (A/Egypt/14724-NAMRU3/2006(H... 38.2 0.17
gb|EF165588.1| Influenza A virus (A/great crested grebe/Bavar... 38.2 0.17
gb|EF165578.1| Influenza A virus (A/mute swan/Bavaria/12/2006... 38.2 0.17
gb|CY017045.1| Influenza A virus (A/swan/Slovenia/760/2006(H5... 38.2 0.17
gb|CY016901.1| Influenza A virus (A/duck/Egypt/2253-3/2006(H5... 38.2 0.17
gb|DQ659680.1| Influenza A virus (A/common bussard/Bavaria/2/... 38.2 0.17
gb|DQ534021.1| Influenza A virus A/Cygnus olor/Czech Republic... 38.2 0.17

[HenryN]

gb|FJ538949.1| Influenza A virus (A/chicken/Vietnam/NCVD-093/... 40.1 0.044
gb|FJ688369.1| Influenza A virus (A/chicken/Egypt/24NLQP-CLEV... 40.1 0.044
gb|FJ688367.1| Influenza A virus (A/chicken/Egypt/17NLQP-CLEV... 40.1 0.044
gb|FJ688365.1| Influenza A virus (A/chicken/Egypt/10NLQP-CLEV... 40.1 0.044
gb|FJ688364.1| Influenza A virus (A/chicken/Egypt/4NLQP-CLEVB... 40.1 0.044
gb|FJ461658.1| Influenza A virus (A/Egypt/3300-NAMRU3/2008(H5... 40.1 0.044
gb|EU717858.1| Influenza A virus (A/chicken/Egypt/1709-6/2008... 40.1 0.044
gb|EU717856.1| Influenza A virus (A/chicken/Egypt/1709-5/2008... 40.1 0.044
gb|EU574921.1| Influenza A virus (A/chicken/Israel/1055/2008(... 40.1 0.044
gb|EU430497.1| Influenza A virus (A/domestic green-winged tea... 40.1 0.044
gb|EU430510.1| Influenza A virus (A/domestic green-winged tea... 40.1 0.044
gb|EF587279.1| Influenza A virus (A/Beijing/01/2003(H5N1)) se... 40.1 0.044
gb|EF124329.1| Influenza A virus (A/goose/Yunnan/3644/2005(H5... 40.1 0.044
gb|EF124310.1| Influenza A virus (A/goose/Yunnan/3315/2005(H5... 40.1 0.044
gb|DQ997539.1| Influenza A virus (A/chicken/Jilin/xv/2002(H5N... 40.1 0.044
gb|DQ997378.1| Influenza A virus (A/chicken/Jilin/hf/2002(H5N... 40.1 0.044
gb|DQ997284.1| Influenza A virus (A/chicken/Jilin/hd/2002(H5N... 40.1 0.044
gb|AY653195.1| Influenza A virus (A/chicken/Jilin/9/2004(H5N1... 40.1 0.044