tetano
Editor, Senior Moderator
Chembiochem. 2017 May 30. doi: 10.1002/cbic.201700271. [Epub ahead of print]
[h=1]A fluorescent RNA forced-intercalation probe as a pan-selective marker for influenza A virus infection.[/h] Haralampiev I[SUP]1[/SUP], Schade M[SUP]1[/SUP], Chamiolo J[SUP]2[/SUP], Jolmes F[SUP]1[/SUP], Prisner S[SUP]1[/SUP], Witkowski P[SUP]3[/SUP], Behrent M[SUP]1[/SUP], H?velmann F[SUP]1[/SUP], Wolff T[SUP]4[/SUP], Seitz O[SUP]1[/SUP], Herrmann A[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus (IAV) genome is segmented into eight viral ribonucleoproteins, each expressing a negatively orientated viral RNA (vRNA). Along the infection cycle, highly abundant single-stranded small viral RNAs (svRNA) are transcribed in a segment-specific manner. The sequences of the vRNA 5'ends and of svRNAs are identical and highly conserved among all IAV strains. Here we demonstrate that these sequences can be used as a target for a pan-selective sensor of IAV infection. To this end, we used a complementary fluorescent forced-intercalation RNA (IAV QB-FIT) probe with a single locked nucleic acid substitution to increase brightness. We demonstrated by fluorescence in situ hybridization (FISH) that this probe is suitable and easy to use to detect infection of different cell types by a broad variety of avian, porcine and human IAV strains, but not by other influenza virus types. IAV QB-FIT also provides a useful tool to characterize different infection states of the host cell.
? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] Influenza virus; Oligonucleotides; fluorescence in situ hybridization; fluorescent probe; viral RNA
PMID: 28557173 DOI: 10.1002/cbic.201700271
[h=1]A fluorescent RNA forced-intercalation probe as a pan-selective marker for influenza A virus infection.[/h] Haralampiev I[SUP]1[/SUP], Schade M[SUP]1[/SUP], Chamiolo J[SUP]2[/SUP], Jolmes F[SUP]1[/SUP], Prisner S[SUP]1[/SUP], Witkowski P[SUP]3[/SUP], Behrent M[SUP]1[/SUP], H?velmann F[SUP]1[/SUP], Wolff T[SUP]4[/SUP], Seitz O[SUP]1[/SUP], Herrmann A[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus (IAV) genome is segmented into eight viral ribonucleoproteins, each expressing a negatively orientated viral RNA (vRNA). Along the infection cycle, highly abundant single-stranded small viral RNAs (svRNA) are transcribed in a segment-specific manner. The sequences of the vRNA 5'ends and of svRNAs are identical and highly conserved among all IAV strains. Here we demonstrate that these sequences can be used as a target for a pan-selective sensor of IAV infection. To this end, we used a complementary fluorescent forced-intercalation RNA (IAV QB-FIT) probe with a single locked nucleic acid substitution to increase brightness. We demonstrated by fluorescence in situ hybridization (FISH) that this probe is suitable and easy to use to detect infection of different cell types by a broad variety of avian, porcine and human IAV strains, but not by other influenza virus types. IAV QB-FIT also provides a useful tool to characterize different infection states of the host cell.
? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] Influenza virus; Oligonucleotides; fluorescence in situ hybridization; fluorescent probe; viral RNA
PMID: 28557173 DOI: 10.1002/cbic.201700271