tetano
Editor, Senior Moderator
Methods. 2012 Oct 1. pii: S1046-2023(12)00249-6. doi: 10.1016/j.ymeth.2012.09.009. [Epub ahead of print]
A Genome Wide RNA interference Screening Method to Identify Host Factors that Modulate Influenza A Virus Replication.
Chin CR, Brass AL.
Source
Microbiology and Physiological Systems Department, University of Massachusetts Medical School, Worcester, MA, 01655.
Abstract
The use of genome wide RNA interference (RNAi) screens to investigate host-viral interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
23036328
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23036328
A Genome Wide RNA interference Screening Method to Identify Host Factors that Modulate Influenza A Virus Replication.
Chin CR, Brass AL.
Source
Microbiology and Physiological Systems Department, University of Massachusetts Medical School, Worcester, MA, 01655.
Abstract
The use of genome wide RNA interference (RNAi) screens to investigate host-viral interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
23036328
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23036328