tetano
Editor, Senior Moderator
J Med Virol. 2014 Apr 24. doi: 10.1002/jmv.23955. [Epub ahead of print]
A novel molecular test for influenza B virus detection and lineage differentiation.
Wong CK1, Tsang GC, Chan KH, Li OT, Peiris M, Poon LL.
Author information
Abstract
Contemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance. J. Med. Virol. ? 2014 Wiley Periodicals, Inc.
? 2014 Wiley Periodicals, Inc.
KEYWORDS:
RT-PCR, Victoria, Yamagata, influenza B virus, lineage differentiation
PMID:
24760697
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24760697
A novel molecular test for influenza B virus detection and lineage differentiation.
Wong CK1, Tsang GC, Chan KH, Li OT, Peiris M, Poon LL.
Author information
Abstract
Contemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance. J. Med. Virol. ? 2014 Wiley Periodicals, Inc.
? 2014 Wiley Periodicals, Inc.
KEYWORDS:
RT-PCR, Victoria, Yamagata, influenza B virus, lineage differentiation
PMID:
24760697
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24760697