tetano
Editor, Senior Moderator
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Feb;27(1):1-4.
[Accessing the features of surface neuraminidase (N1) of influenza A virus presenting on the platforms for anti-NA Abs screening].
[Article in Chinese]
Huang L, Qin K, Zhou JF, Shu YL, Wei H.
Source
Children's Hospital of Changqing Medical University, China.
Abstract
OBJECTIVE:
To understand if the Neuraminidase (N1) of Influenza A virus at the surface of yeast-displaying system, eukaryotic expression system and the infected cells could be used for anti-NA Abs screening, their activities and bindings to five candidate Abs were assayed.
METHODS:
The surface NA expression was obtained by transfecting by recombinant NA constructors with specific tag-labels or live virus infection. The functional activity was measured by the fluorescent assay. Their bindings to the Abs were detected by flow cytometry.
RESULTS:
The surface NAs presenting on the yeast-displaying system and eukaryotic expression system exhibited functional NA activities as the NA at the surface of virus-infected cells which showed affinities to Ab1, 4, and 5. The same bindings to Abl and 5 were found in the surface NA expressed by eukaryotic expression system while minor binding was observed in the yeast displayed-NA.
CONCLUSION:
The epitopes of yeast-displayed NA may be different from the NAs present at eukaryotic expression system and the infected cells which more likely suitable for the screening of anti-NA Abs.
PMID:
23855116
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23855116
[Accessing the features of surface neuraminidase (N1) of influenza A virus presenting on the platforms for anti-NA Abs screening].
[Article in Chinese]
Huang L, Qin K, Zhou JF, Shu YL, Wei H.
Source
Children's Hospital of Changqing Medical University, China.
Abstract
OBJECTIVE:
To understand if the Neuraminidase (N1) of Influenza A virus at the surface of yeast-displaying system, eukaryotic expression system and the infected cells could be used for anti-NA Abs screening, their activities and bindings to five candidate Abs were assayed.
METHODS:
The surface NA expression was obtained by transfecting by recombinant NA constructors with specific tag-labels or live virus infection. The functional activity was measured by the fluorescent assay. Their bindings to the Abs were detected by flow cytometry.
RESULTS:
The surface NAs presenting on the yeast-displaying system and eukaryotic expression system exhibited functional NA activities as the NA at the surface of virus-infected cells which showed affinities to Ab1, 4, and 5. The same bindings to Abl and 5 were found in the surface NA expressed by eukaryotic expression system while minor binding was observed in the yeast displayed-NA.
CONCLUSION:
The epitopes of yeast-displayed NA may be different from the NAs present at eukaryotic expression system and the infected cells which more likely suitable for the screening of anti-NA Abs.
PMID:
23855116
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23855116