tetano
Editor, Senior Moderator
ACS Appl Mater Interfaces
. 2022 Oct 27.
doi: 10.1021/acsami.2c14497. Online ahead of print.
MnO[SUB]2[/SUB] Nanozyme-Mediated CRISPR-Cas12a System for the Detection of SARS-CoV-2
Lina Wu[SUP] 1 2 [/SUP], Xinjie Wang[SUP] 3 [/SUP], Xiangchuan Wu[SUP] 1 [/SUP], Shiqi Xu[SUP] 1 [/SUP], Ming Liu[SUP] 4 [/SUP], Xizhong Cao[SUP] 4 [/SUP], Taishan Tang[SUP] 4 [/SUP], Xingxu Huang[SUP] 2 [/SUP], He Huang[SUP] 1 [/SUP]
Affiliations
Abstract
The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their low cost of use and user-friendly operation. Here, a MnO[SUB]2[/SUB] nanozyme-mediated CRISPR-Cas12a system was instituted to detect SARS-CoV-2. MnO[SUB]2[/SUB] nanorods linked to magnetic beads via a single-stranded DNA (ssDNA) linker used as an oxidase-like nanozyme inducing the color change of 3,3',5,5'-tetramethylbenzidine, which can be distinguished by the naked eye. The detection buffer color will change when the Cas12a is activated by SARS-CoV-2 and indiscriminately cleave the linker ssDNA. The detection limit was 10 copies per microliter and showed no cross-reaction with other coronaviruses. The nanozyme-mediated CRISPR-Cas12a system shows high selectivity and facile operation, with great potential for molecular diagnosis in point-of-care testing applications.
Keywords: CRISPR-Cas12a; Detection; MnO2 nanorods; Nanozyme; SARS-CoV-2.
. 2022 Oct 27.
doi: 10.1021/acsami.2c14497. Online ahead of print.
MnO[SUB]2[/SUB] Nanozyme-Mediated CRISPR-Cas12a System for the Detection of SARS-CoV-2
Lina Wu[SUP] 1 2 [/SUP], Xinjie Wang[SUP] 3 [/SUP], Xiangchuan Wu[SUP] 1 [/SUP], Shiqi Xu[SUP] 1 [/SUP], Ming Liu[SUP] 4 [/SUP], Xizhong Cao[SUP] 4 [/SUP], Taishan Tang[SUP] 4 [/SUP], Xingxu Huang[SUP] 2 [/SUP], He Huang[SUP] 1 [/SUP]
Affiliations
- PMID: 36301087
- DOI: 10.1021/acsami.2c14497
Abstract
The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their low cost of use and user-friendly operation. Here, a MnO[SUB]2[/SUB] nanozyme-mediated CRISPR-Cas12a system was instituted to detect SARS-CoV-2. MnO[SUB]2[/SUB] nanorods linked to magnetic beads via a single-stranded DNA (ssDNA) linker used as an oxidase-like nanozyme inducing the color change of 3,3',5,5'-tetramethylbenzidine, which can be distinguished by the naked eye. The detection buffer color will change when the Cas12a is activated by SARS-CoV-2 and indiscriminately cleave the linker ssDNA. The detection limit was 10 copies per microliter and showed no cross-reaction with other coronaviruses. The nanozyme-mediated CRISPR-Cas12a system shows high selectivity and facile operation, with great potential for molecular diagnosis in point-of-care testing applications.
Keywords: CRISPR-Cas12a; Detection; MnO2 nanorods; Nanozyme; SARS-CoV-2.