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Antiviral Res . Coronavirus Nucleocapsid-based Vaccine Provides Partial Protection against Hetero-species Coronavirus in Murine Models

tetano

Editor, Senior Moderator
Antiviral Res


. 2024 Aug 22:105991.
doi: 10.1016/j.antiviral.2024.105991. Online ahead of print. Coronavirus Nucleocapsid-based Vaccine Provides Partial Protection against Hetero-species Coronavirus in Murine Models

Pureum Lee[SUP] 1 [/SUP], Jihee Kim[SUP] 2 [/SUP], Hanseul Oh[SUP] 3 [/SUP], Chang-Ung Kim[SUP] 4 [/SUP], Ahn Young Jeong[SUP] 5 [/SUP], Moo-Seung Lee[SUP] 1 [/SUP], Min Seong Jang[SUP] 6 [/SUP], Jung Joo Hong[SUP] 7 [/SUP], Jung-Eun Park[SUP] 8 [/SUP], Doo-Jin Kim[SUP] 9 [/SUP]



Affiliations
Abstract

Most coronavirus vaccines focus on the spike (S) antigen, but the frequent mutations in S raise concerns about the vaccine efficacy against new variants. Although additional antigens with conserved sequences are have been tested, the extent to which these vaccines can provide immunity against different coronavirus species remains unclear. In this study, we assessed the potential of nucleocapsid (N) as a coronavirus vaccine antigen. Immunization with MERS-CoV N induced robust immune responses, providing significant protection against MERS-CoV. Notably, MERS-CoV N elicited cross-reactive T cell responses to SARS-CoV-2 N and significantly reduced lung inflammation following a SARS-CoV-2 challenge in the transient hACE2 mouse model. However, in K18-hACE transgenic mice, the vaccine showed limited protection. Collectively, our findings suggest that coronavirus N can be an effective vaccine antigen against homologous viruses, but its efficacy may vary across different coronaviruses, highlighting the need for further research on pan-coronavirus vaccines using conserved antigens.

Keywords: Coronavirus; Cross-protection; Nucleocapsid; Vaccine.

 
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