tetano
Editor, Senior Moderator
Bull Exp Biol Med
. 2025 Sep 25.
doi: 10.1007/s10517-025-06482-z. Online ahead of print. Production of Recombinant Nucleocapsid Protein SARSN1 and Its Use for Assessing the Immune Response in Mice after Vaccination with a Live Probiotic Vaccine Containing the Coronavirus Nucleocapsid Protein
T V Gupalova[SUP] 1 [/SUP], E A Bormotova[SUP] 2 [/SUP], G F Leontieva[SUP] 2 [/SUP], T A Kramskaya[SUP] 2 [/SUP], Yu A Desheva[SUP] 2 [/SUP], A N Suvorov[SUP] 2 [/SUP]
Affiliations
The nucleocapsid (N) protein of the severe acute respiratory syndrome coronavirus (SARSCoV) is the most abundant structural protein in virus-infected cells. Its primary function is to pack ~30 kb positive-sense viral RNA genome that includes a 5' cap into a ribonucleoprotein (RNP) complex known as capsid. Among the 4 major structural proteins of SARS-CoV, the N protein exhibits the highest expression levels in the host cells. We produced a recombinant SARS-CoV N protein (SARSN1) and used it to detect specific antibodies in mice vaccinated with a live probiotic vaccine containing the coronavirus nucleocapsid protein.
Keywords: SARS-CoV coronavirus; live probiotic vaccine; recombinant nucleocapsid (N) protein.
. 2025 Sep 25.
doi: 10.1007/s10517-025-06482-z. Online ahead of print. Production of Recombinant Nucleocapsid Protein SARSN1 and Its Use for Assessing the Immune Response in Mice after Vaccination with a Live Probiotic Vaccine Containing the Coronavirus Nucleocapsid Protein
T V Gupalova[SUP] 1 [/SUP], E A Bormotova[SUP] 2 [/SUP], G F Leontieva[SUP] 2 [/SUP], T A Kramskaya[SUP] 2 [/SUP], Yu A Desheva[SUP] 2 [/SUP], A N Suvorov[SUP] 2 [/SUP]
Affiliations
- PMID: 40996646
- DOI: 10.1007/s10517-025-06482-z
The nucleocapsid (N) protein of the severe acute respiratory syndrome coronavirus (SARSCoV) is the most abundant structural protein in virus-infected cells. Its primary function is to pack ~30 kb positive-sense viral RNA genome that includes a 5' cap into a ribonucleoprotein (RNP) complex known as capsid. Among the 4 major structural proteins of SARS-CoV, the N protein exhibits the highest expression levels in the host cells. We produced a recombinant SARS-CoV N protein (SARSN1) and used it to detect specific antibodies in mice vaccinated with a live probiotic vaccine containing the coronavirus nucleocapsid protein.
Keywords: SARS-CoV coronavirus; live probiotic vaccine; recombinant nucleocapsid (N) protein.