tetano
Editor, Senior Moderator
Cell
. 2023 Sep 26;S0092-8674(23)00978-9.
doi: 10.1016/j.cell.2023.08.044. Online ahead of print. Multi-omics analysis of mucosal and systemic immunity to SARS-CoV-2 after birth
Florian Wimmers[SUP] 1 [/SUP], Allison R Burrell[SUP] 2 [/SUP], Yupeng Feng[SUP] 3 [/SUP], Hong Zheng[SUP] 4 [/SUP], Prabhu S Arunachalam[SUP] 3 [/SUP], Mengyun Hu[SUP] 3 [/SUP], Sara Spranger[SUP] 5 [/SUP], Lindsay E Nyhoff[SUP] 6 [/SUP], Devyani Joshi[SUP] 6 [/SUP], Meera Trisal[SUP] 3 [/SUP], Mayanka Awasthi[SUP] 7 [/SUP], Lorenza Bellusci[SUP] 7 [/SUP], Usama Ashraf[SUP] 8 [/SUP], Sangeeta Kowli[SUP] 9 [/SUP], Katherine C Konvinse[SUP] 10 [/SUP], Emily Yang[SUP] 11 [/SUP], Michael Blanco[SUP] 12 [/SUP], Kathryn Pellegrini[SUP] 13 [/SUP], Gregory Tharp[SUP] 13 [/SUP], Thomas Hagan[SUP] 14 [/SUP], R Sharon Chinthrajah[SUP] 15 [/SUP], Tran T Nguyen[SUP] 9 [/SUP], Alba Grifoni[SUP] 16 [/SUP], Alessandro Sette[SUP] 17 [/SUP], Kari C Nadeau[SUP] 15 [/SUP], David B Haslam[SUP] 14 [/SUP], Steven E Bosinger[SUP] 18 [/SUP], Jens Wrammert[SUP] 6 [/SUP], Holden T Maecker[SUP] 9 [/SUP], Paul J Utz[SUP] 11 [/SUP], Taia T Wang[SUP] 19 [/SUP], Surender Khurana[SUP] 7 [/SUP], Purvesh Khatri[SUP] 4 [/SUP], Mary A Staat[SUP] 14 [/SUP], Bali Pulendran[SUP] 20 [/SUP]
Affiliations
The dynamics of immunity to infection in infants remain obscure. Here, we used a multi-omics approach to perform a longitudinal analysis of immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in infants and young children by analyzing blood samples and weekly nasal swabs collected before, during, and after infection with Omicron and non-Omicron variants. Infection stimulated robust antibody titers that, unlike in adults, showed no sign of decay for up to 300 days. Infants mounted a robust mucosal immune response characterized by inflammatory cytokines, interferon (IFN) α, and T helper (Th) 17 and neutrophil markers (interleukin [IL]-17, IL-8, and CXCL1). The immune response in blood was characterized by upregulation of activation markers on innate cells, no inflammatory cytokines, but several chemokines and IFNα. The latter correlated with viral load and expression of interferon-stimulated genes (ISGs) in myeloid cells measured by single-cell multi-omics. Together, these data provide a snapshot of immunity to infection during the initial weeks and months of life.
Keywords: SARS-CoV-2; durability; infants; mucosal immunity; multi-omics; neonates; single-cell ATAC-seq; single-cell RNA-seq; viral infection.
. 2023 Sep 26;S0092-8674(23)00978-9.
doi: 10.1016/j.cell.2023.08.044. Online ahead of print. Multi-omics analysis of mucosal and systemic immunity to SARS-CoV-2 after birth
Florian Wimmers[SUP] 1 [/SUP], Allison R Burrell[SUP] 2 [/SUP], Yupeng Feng[SUP] 3 [/SUP], Hong Zheng[SUP] 4 [/SUP], Prabhu S Arunachalam[SUP] 3 [/SUP], Mengyun Hu[SUP] 3 [/SUP], Sara Spranger[SUP] 5 [/SUP], Lindsay E Nyhoff[SUP] 6 [/SUP], Devyani Joshi[SUP] 6 [/SUP], Meera Trisal[SUP] 3 [/SUP], Mayanka Awasthi[SUP] 7 [/SUP], Lorenza Bellusci[SUP] 7 [/SUP], Usama Ashraf[SUP] 8 [/SUP], Sangeeta Kowli[SUP] 9 [/SUP], Katherine C Konvinse[SUP] 10 [/SUP], Emily Yang[SUP] 11 [/SUP], Michael Blanco[SUP] 12 [/SUP], Kathryn Pellegrini[SUP] 13 [/SUP], Gregory Tharp[SUP] 13 [/SUP], Thomas Hagan[SUP] 14 [/SUP], R Sharon Chinthrajah[SUP] 15 [/SUP], Tran T Nguyen[SUP] 9 [/SUP], Alba Grifoni[SUP] 16 [/SUP], Alessandro Sette[SUP] 17 [/SUP], Kari C Nadeau[SUP] 15 [/SUP], David B Haslam[SUP] 14 [/SUP], Steven E Bosinger[SUP] 18 [/SUP], Jens Wrammert[SUP] 6 [/SUP], Holden T Maecker[SUP] 9 [/SUP], Paul J Utz[SUP] 11 [/SUP], Taia T Wang[SUP] 19 [/SUP], Surender Khurana[SUP] 7 [/SUP], Purvesh Khatri[SUP] 4 [/SUP], Mary A Staat[SUP] 14 [/SUP], Bali Pulendran[SUP] 20 [/SUP]
Affiliations
- PMID: 37776858
- DOI: 10.1016/j.cell.2023.08.044
The dynamics of immunity to infection in infants remain obscure. Here, we used a multi-omics approach to perform a longitudinal analysis of immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in infants and young children by analyzing blood samples and weekly nasal swabs collected before, during, and after infection with Omicron and non-Omicron variants. Infection stimulated robust antibody titers that, unlike in adults, showed no sign of decay for up to 300 days. Infants mounted a robust mucosal immune response characterized by inflammatory cytokines, interferon (IFN) α, and T helper (Th) 17 and neutrophil markers (interleukin [IL]-17, IL-8, and CXCL1). The immune response in blood was characterized by upregulation of activation markers on innate cells, no inflammatory cytokines, but several chemokines and IFNα. The latter correlated with viral load and expression of interferon-stimulated genes (ISGs) in myeloid cells measured by single-cell multi-omics. Together, these data provide a snapshot of immunity to infection during the initial weeks and months of life.
Keywords: SARS-CoV-2; durability; infants; mucosal immunity; multi-omics; neonates; single-cell ATAC-seq; single-cell RNA-seq; viral infection.