tetano
Editor, Senior Moderator
J Virol. 2012 Jun 20. [Epub ahead of print]
Characterization in vitro and in vivo of pandemic (H1N1) 2009 viruses isolated from patients.
Watanabe T, Imai M, Watanabe S, Shinya K, Hatta M, Li C, Neumann G, Ozawa M, Hanson A, Zhong G, Fukuyama S, Kawakami E, Simmons HA, Schenkman D, Brunner K, Capuano SV 3rd, Weinfurter JT, Kilander A, Dudman SG, Suresh M, Hungnes O, Friedrich TC, Kawaoka Y.
Source
Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53711, USA.
Abstract
The first influenza pandemic of the 21(st) century was caused by novel H1N1 viruses that emerged in early 2009. Molecular evolutionary analyses of the pandemic influenza A H1N1 [A(H1N1)pdm09] virus revealed two major clusters, Cluster I and Cluster II. Although the pathogenicity of viruses belonging to Cluster I, which became extinct by the end of 2009, has been examined in a nonhuman primate model, the pathogenic potential of viruses belonging to Cluster II, which has spread more widely in the world, has not been studied in this animal model. Here, we characterized two Norwegian isolates belonging to Cluster II, namely A/Norway/3568/2009 (Norway3568) and A/Norway/3487-2/2009 (Norway3487), which caused distinct clinical symptoms despite their genetic similarity. We observed more efficient replication in cultured cells and delayed virus clearance from ferret respiratory organs for Norway3487 virus, which was isolated from a severe case, as compared to Norway3568, which was isolated from a mild case. Moreover, Norway3487 virus caused to some extent more severe lung damage in nonhuman primates than did Norway3568 virus. Our data suggest that the distinct replicative and pathogenic potential of these two viruses may result from differences in their biological properties (e.g., the receptor-binding specificity of hemagglutinin and viral polymerase activity).
PMID:
22718834
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22718834
Characterization in vitro and in vivo of pandemic (H1N1) 2009 viruses isolated from patients.
Watanabe T, Imai M, Watanabe S, Shinya K, Hatta M, Li C, Neumann G, Ozawa M, Hanson A, Zhong G, Fukuyama S, Kawakami E, Simmons HA, Schenkman D, Brunner K, Capuano SV 3rd, Weinfurter JT, Kilander A, Dudman SG, Suresh M, Hungnes O, Friedrich TC, Kawaoka Y.
Source
Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53711, USA.
Abstract
The first influenza pandemic of the 21(st) century was caused by novel H1N1 viruses that emerged in early 2009. Molecular evolutionary analyses of the pandemic influenza A H1N1 [A(H1N1)pdm09] virus revealed two major clusters, Cluster I and Cluster II. Although the pathogenicity of viruses belonging to Cluster I, which became extinct by the end of 2009, has been examined in a nonhuman primate model, the pathogenic potential of viruses belonging to Cluster II, which has spread more widely in the world, has not been studied in this animal model. Here, we characterized two Norwegian isolates belonging to Cluster II, namely A/Norway/3568/2009 (Norway3568) and A/Norway/3487-2/2009 (Norway3487), which caused distinct clinical symptoms despite their genetic similarity. We observed more efficient replication in cultured cells and delayed virus clearance from ferret respiratory organs for Norway3487 virus, which was isolated from a severe case, as compared to Norway3568, which was isolated from a mild case. Moreover, Norway3487 virus caused to some extent more severe lung damage in nonhuman primates than did Norway3568 virus. Our data suggest that the distinct replicative and pathogenic potential of these two viruses may result from differences in their biological properties (e.g., the receptor-binding specificity of hemagglutinin and viral polymerase activity).
PMID:
22718834
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22718834