tetano
Editor, Senior Moderator
Biochem Biophys Res Commun. 2019 Jun 10. pii: S0006-291X(19)31059-9. doi: 10.1016/j.bbrc.2019.05.161. [Epub ahead of print]
[h=1]Characterization of influenza virus PR8 strain cultured in embryonated eggs by cryo-electron tomography.[/h] Chen Q[SUP]1[/SUP], Huang X[SUP]2[/SUP], Wei R[SUP]2[/SUP], Zhang L[SUP]3[/SUP], Yin C[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses, as causative agents of seasonal epidemics and periodic worldwide pandemics, cause enormous mortality loss globally. The PR8 strain cultured in chicken eggs is widely used for scientific research and the production of influenza vaccines. Here, based on Cryo-electron Tomography (CET), we analyzed the morphological and structural characteristics of the influenza virus PR8 strain at different pHs. We found that a large number of defective virions were propagated in embryonated eggs. By comparing virions with/without the matrix layer, it was revealed that the matrix layer played an essential role in the structural integrity of virions and RNPs encapsulation during the influenza virus life cycle. We also utilized hemagglutinin receptor-containing liposomes to mimic the membrane fusion process. Several potential intermediates of HA during membrane fusion were observed at acidic pH. Our observations afford insight into the architecture and function of influenza virus.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cryo-electron microscopy; Influenza virus; PR8 strain; Virus structure
PMID: 31196621 DOI: 10.1016/j.bbrc.2019.05.161
[h=1]Characterization of influenza virus PR8 strain cultured in embryonated eggs by cryo-electron tomography.[/h] Chen Q[SUP]1[/SUP], Huang X[SUP]2[/SUP], Wei R[SUP]2[/SUP], Zhang L[SUP]3[/SUP], Yin C[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses, as causative agents of seasonal epidemics and periodic worldwide pandemics, cause enormous mortality loss globally. The PR8 strain cultured in chicken eggs is widely used for scientific research and the production of influenza vaccines. Here, based on Cryo-electron Tomography (CET), we analyzed the morphological and structural characteristics of the influenza virus PR8 strain at different pHs. We found that a large number of defective virions were propagated in embryonated eggs. By comparing virions with/without the matrix layer, it was revealed that the matrix layer played an essential role in the structural integrity of virions and RNPs encapsulation during the influenza virus life cycle. We also utilized hemagglutinin receptor-containing liposomes to mimic the membrane fusion process. Several potential intermediates of HA during membrane fusion were observed at acidic pH. Our observations afford insight into the architecture and function of influenza virus.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cryo-electron microscopy; Influenza virus; PR8 strain; Virus structure
PMID: 31196621 DOI: 10.1016/j.bbrc.2019.05.161