tetano
Editor, Senior Moderator
Front Immunol. 2019 Nov 20;10:2621. doi: 10.3389/fimmu.2019.02621. eCollection 2019. [h=1]Characterization of the Dynamic Behavior of Neutrophils Following Influenza Vaccination.[/h]
Pizzagalli DU[SUP]1,[/SUP][SUP]2[/SUP], Latino I[SUP]1[/SUP], Pulfer A[SUP]1[/SUP], Palomino-Segura M[SUP]1[/SUP], Virgilio T[SUP]1[/SUP], Farsakoglu Y[SUP]3[/SUP], Krause R[SUP]2[/SUP], Gonzalez SF[SUP]1[/SUP].
[h=3]Author information[/h] 1 Institute for Research in Biomedicine, Universit? della Svizzera italiana, Bellinzona, Switzerland. 2 Institute of Computational Science, Universit? della Svizzera italiana, Lugano, Switzerland. 3 Salk Institute, San Diego, CA, United States.
[h=3]Abstract[/h] Neutrophils are amongst the first cells to respond to inflammation and infection. Although they play a key role in limiting the dissemination of pathogens, the study of their dynamic behavior in immune organs remains elusive. In this work, we characterized in vivo the dynamic behavior of neutrophils in the mouse popliteal lymph node (PLN) after influenza vaccination with UV-inactivated virus. To achieve this, we used an image-based systems biology approach to detect the motility patterns of neutrophils and to associate them to distinct actions. We described a prominent and rapid recruitment of neutrophils to the PLN following vaccination, which was dependent on the secretion of the chemokine CXCL1 and the alarmin molecule IL-1α. In addition, we observed that the initial recruitment occurred mainly via high endothelial venules located in the paracortical and interfollicular regions of the PLN. The analysis of the spatial-temporal patterns of neutrophil migration demonstrated that, in the initial stage, the majority of neutrophils displayed a patrolling behavior, followed by the formation of swarms in the subcapsular sinus of the PLN, which were associated with macrophages in this compartment. Finally, we observed using multiple imaging techniques, that neutrophils phagocytize and transport influenza virus particles. These processes might have important implications in the capacity of these cells to present viral antigens.
Copyright ? 2019 Pizzagalli, Latino, Pulfer, Palomino-Segura, Virgilio, Farsakoglu, Krause and Gonzalez.
[h=4]KEYWORDS:[/h] action recognition; cell actions; data mining; innate immunity; intravital 2-photon; neutrophils; vaccination
PMID: 31824481 PMCID: PMC6881817 DOI: 10.3389/fimmu.2019.02621
Free PMC Article
Pizzagalli DU[SUP]1,[/SUP][SUP]2[/SUP], Latino I[SUP]1[/SUP], Pulfer A[SUP]1[/SUP], Palomino-Segura M[SUP]1[/SUP], Virgilio T[SUP]1[/SUP], Farsakoglu Y[SUP]3[/SUP], Krause R[SUP]2[/SUP], Gonzalez SF[SUP]1[/SUP].
[h=3]Author information[/h] 1 Institute for Research in Biomedicine, Universit? della Svizzera italiana, Bellinzona, Switzerland. 2 Institute of Computational Science, Universit? della Svizzera italiana, Lugano, Switzerland. 3 Salk Institute, San Diego, CA, United States.
[h=3]Abstract[/h] Neutrophils are amongst the first cells to respond to inflammation and infection. Although they play a key role in limiting the dissemination of pathogens, the study of their dynamic behavior in immune organs remains elusive. In this work, we characterized in vivo the dynamic behavior of neutrophils in the mouse popliteal lymph node (PLN) after influenza vaccination with UV-inactivated virus. To achieve this, we used an image-based systems biology approach to detect the motility patterns of neutrophils and to associate them to distinct actions. We described a prominent and rapid recruitment of neutrophils to the PLN following vaccination, which was dependent on the secretion of the chemokine CXCL1 and the alarmin molecule IL-1α. In addition, we observed that the initial recruitment occurred mainly via high endothelial venules located in the paracortical and interfollicular regions of the PLN. The analysis of the spatial-temporal patterns of neutrophil migration demonstrated that, in the initial stage, the majority of neutrophils displayed a patrolling behavior, followed by the formation of swarms in the subcapsular sinus of the PLN, which were associated with macrophages in this compartment. Finally, we observed using multiple imaging techniques, that neutrophils phagocytize and transport influenza virus particles. These processes might have important implications in the capacity of these cells to present viral antigens.
Copyright ? 2019 Pizzagalli, Latino, Pulfer, Palomino-Segura, Virgilio, Farsakoglu, Krause and Gonzalez.
[h=4]KEYWORDS:[/h] action recognition; cell actions; data mining; innate immunity; intravital 2-photon; neutrophils; vaccination
PMID: 31824481 PMCID: PMC6881817 DOI: 10.3389/fimmu.2019.02621
Free PMC Article