tetano
Editor, Senior Moderator
Clin Lab Med
. 2022 Mar;42(1):57-73.
doi: 10.1016/j.cll.2021.10.007. Epub 2021 Nov 3.
Alternative Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies
Rashmi Patel[SUP] 1 [/SUP], Siddharth Khare[SUP] 1 [/SUP], Vinay S Mahajan[SUP] 2 [/SUP]
Affiliations
Abstract
The COVID-19 pandemic has resulted in the development, validation, and rapid adoption of multiple novel diagnostic approaches. Hundreds of SARS-CoV-2 serologic assays have been developed and deployed to contain the spread of the virus, and to supply timely and important health information. Most of these serologic assays were based on a conventional enzyme-linked immunosorbent assay or the lateral flow assay format. The immunoassays that were developed were based on alternative technologies and are highlighted in this article with a brief discussion of the assay principle and the pros and cons for each assay. Measurement of neutralizing antibodies is also discussed.
Keywords: ELISA; Electrochemical detection; Immunoassay; Luminescence; Neutralization assay; SARS-CoV-2; Serology.
. 2022 Mar;42(1):57-73.
doi: 10.1016/j.cll.2021.10.007. Epub 2021 Nov 3.
Alternative Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies
Rashmi Patel[SUP] 1 [/SUP], Siddharth Khare[SUP] 1 [/SUP], Vinay S Mahajan[SUP] 2 [/SUP]
Affiliations
- PMID: 35153048
- DOI: 10.1016/j.cll.2021.10.007
Abstract
The COVID-19 pandemic has resulted in the development, validation, and rapid adoption of multiple novel diagnostic approaches. Hundreds of SARS-CoV-2 serologic assays have been developed and deployed to contain the spread of the virus, and to supply timely and important health information. Most of these serologic assays were based on a conventional enzyme-linked immunosorbent assay or the lateral flow assay format. The immunoassays that were developed were based on alternative technologies and are highlighted in this article with a brief discussion of the assay principle and the pros and cons for each assay. Measurement of neutralizing antibodies is also discussed.
Keywords: ELISA; Electrochemical detection; Immunoassay; Luminescence; Neutralization assay; SARS-CoV-2; Serology.