tetano
Editor, Senior Moderator
J Infect Chemother. 2019 Sep 23. pii: S1341-321X(19)30264-8. doi: 10.1016/j.jiac.2019.08.015. [Epub ahead of print] [h=1]Clinical evaluation of ID NOW influenza A & B 2, a rapid influenza virus detection kit using isothermal nucleic acid amplification technology - A comparison with currently available tests.[/h]
Mitamura K[SUP]1[/SUP], Shimizu H[SUP]2[/SUP], Yamazaki M[SUP]3[/SUP], Ichikawa M[SUP]4[/SUP], Abe T[SUP]5[/SUP], Yasumi Y[SUP]6[/SUP], Ichikawa Y[SUP]7[/SUP], Shibata T[SUP]8[/SUP], Yoshihara M[SUP]9[/SUP], Shiozaki K[SUP]10[/SUP], Baba S[SUP]11[/SUP], Kudo Y[SUP]12[/SUP], Tokushima M[SUP]13[/SUP], Konomi Y[SUP]14[/SUP], Kawakami C[SUP]15[/SUP].
[h=3]Author information[/h] 1 Eiju General Hospital, Taito-ku, Tokyo 110-8645, Japan. Electronic address: mitamura@eijuhp.com. 2 Kawasaki City Institute for Public Health, Kawasaki, Kanagawa 210-0834, Japan. 3 Zama Children's Clinic, Zama, Kanagawa 252-0023, Japan. 4 Ichikawa Children's Clinic, Isehara, Kanagawa 259-1133, Japan. 5 Abe Children's Clinic, Yokohama, Kanagawa, 223-0051, Japan. 6 Yasumi Hospital, Morioka, Iwate 028-4125, Japan. 7 Ichikawa Clinic, Fukushima-shi, Fukushima 960-0112, Japan. 8 Futaba Children's Clinic, Ushiku, Ibaraki 300-1207, Japan. 9 Junseikai Yoshihara Clinic, Takasaki, Gunma 370-3524, Japan. 10 Shiozaki Clinic, Wakayama, Wakayama 640-8482, Japan. 11 Wakaayukai Baba Clinic, Nagaokakyou, Kyoto 617-0814, Japan. 12 Kudo Clinic, Kakogawa, Hyogo 675-0018, Japan. 13 Maebashi Kita Hospital, Maebashi, Gunma 371-0054, Japan. 14 Jinjikai Takahashi Clinic, Bando, Ibaraki 306-0631, Japan. 15 Yokohama City Institute of Public Health, Yokohama, Kanagawa 236-0051, Japan.
[h=3]Abstract[/h] In this study, we evaluated the performance of ID NOW Influenza A & B 2 (ID NOW 2), a rapid molecular point-of-care test for influenza within 13 min, in comparison with currently available tests. A total of 254 nasopharyngeal swabs (NPS) and 271 nasopharyngeal aspirates (NPA) collected from 373 children and 152 adults with influenza-like illness were tested using ID NOW 2, viral culture, rapid antigen detection test, and loop-mediated isothermal amplification test to evaluate the sensitivity and specificity compared with real-time reverse transcription polymerase chain reaction as the reference method. The sensitivities of ID NOW 2 for influenza A were 95.9% and 95.7% in NPS and NPA, respectively, and for influenza B were 100% and 98.7% in NPS and NPA, respectively. The specificity was 100% for both influenza A and influenza B in NPS and NPA. Sensitivity of each test method reflected the difference of analytical sensitivity among the tests, and ID NOW 2 was not affected by time after illness onset and patient age. In conclusion, ID NOW 2 demonstrated a high sensitivity and specificity that is useful for diagnosis of influenza in the clinical setting and infection control.
Copyright ? 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] ID NOW influenza A & B 2; Influenza; Isothermal nucleic acid amplification; Point-of-care testing; Rapid antigen detection test
PMID: 31558351 DOI: 10.1016/j.jiac.2019.08.015
Mitamura K[SUP]1[/SUP], Shimizu H[SUP]2[/SUP], Yamazaki M[SUP]3[/SUP], Ichikawa M[SUP]4[/SUP], Abe T[SUP]5[/SUP], Yasumi Y[SUP]6[/SUP], Ichikawa Y[SUP]7[/SUP], Shibata T[SUP]8[/SUP], Yoshihara M[SUP]9[/SUP], Shiozaki K[SUP]10[/SUP], Baba S[SUP]11[/SUP], Kudo Y[SUP]12[/SUP], Tokushima M[SUP]13[/SUP], Konomi Y[SUP]14[/SUP], Kawakami C[SUP]15[/SUP].
[h=3]Author information[/h] 1 Eiju General Hospital, Taito-ku, Tokyo 110-8645, Japan. Electronic address: mitamura@eijuhp.com. 2 Kawasaki City Institute for Public Health, Kawasaki, Kanagawa 210-0834, Japan. 3 Zama Children's Clinic, Zama, Kanagawa 252-0023, Japan. 4 Ichikawa Children's Clinic, Isehara, Kanagawa 259-1133, Japan. 5 Abe Children's Clinic, Yokohama, Kanagawa, 223-0051, Japan. 6 Yasumi Hospital, Morioka, Iwate 028-4125, Japan. 7 Ichikawa Clinic, Fukushima-shi, Fukushima 960-0112, Japan. 8 Futaba Children's Clinic, Ushiku, Ibaraki 300-1207, Japan. 9 Junseikai Yoshihara Clinic, Takasaki, Gunma 370-3524, Japan. 10 Shiozaki Clinic, Wakayama, Wakayama 640-8482, Japan. 11 Wakaayukai Baba Clinic, Nagaokakyou, Kyoto 617-0814, Japan. 12 Kudo Clinic, Kakogawa, Hyogo 675-0018, Japan. 13 Maebashi Kita Hospital, Maebashi, Gunma 371-0054, Japan. 14 Jinjikai Takahashi Clinic, Bando, Ibaraki 306-0631, Japan. 15 Yokohama City Institute of Public Health, Yokohama, Kanagawa 236-0051, Japan.
[h=3]Abstract[/h] In this study, we evaluated the performance of ID NOW Influenza A & B 2 (ID NOW 2), a rapid molecular point-of-care test for influenza within 13 min, in comparison with currently available tests. A total of 254 nasopharyngeal swabs (NPS) and 271 nasopharyngeal aspirates (NPA) collected from 373 children and 152 adults with influenza-like illness were tested using ID NOW 2, viral culture, rapid antigen detection test, and loop-mediated isothermal amplification test to evaluate the sensitivity and specificity compared with real-time reverse transcription polymerase chain reaction as the reference method. The sensitivities of ID NOW 2 for influenza A were 95.9% and 95.7% in NPS and NPA, respectively, and for influenza B were 100% and 98.7% in NPS and NPA, respectively. The specificity was 100% for both influenza A and influenza B in NPS and NPA. Sensitivity of each test method reflected the difference of analytical sensitivity among the tests, and ID NOW 2 was not affected by time after illness onset and patient age. In conclusion, ID NOW 2 demonstrated a high sensitivity and specificity that is useful for diagnosis of influenza in the clinical setting and infection control.
Copyright ? 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] ID NOW influenza A & B 2; Influenza; Isothermal nucleic acid amplification; Point-of-care testing; Rapid antigen detection test
PMID: 31558351 DOI: 10.1016/j.jiac.2019.08.015