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Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine

tetano

Editor, Senior Moderator
J Immunol. 2018 Apr 27. pii: ji1800142. doi: 10.4049/jimmunol.1800142. [Epub ahead of print]
[h=1]Comparison of Heterosubtypic Protection in Ferrets and Pigs Induced by a Single-Cycle Influenza Vaccine.[/h] Holzer B[SUP]1[/SUP], Morgan SB[SUP]1[/SUP], Matsuoka Y[SUP]2[/SUP], Edmans M[SUP]1[/SUP], Salguero FJ[SUP]3[/SUP], Everett H[SUP]4[/SUP], Brookes SM[SUP]4[/SUP], Porter E[SUP]5[/SUP], MacLoughlin R[SUP]6[/SUP], Charleston B[SUP]1[/SUP], Subbarao K[SUP]2[/SUP], Townsend A[SUP]7[/SUP], Tchilian E[SUP]8[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] Influenza is a major health threat, and a broadly protective influenza vaccine would be a significant advance. Signal Minus FLU (S-FLU) is a candidate broadly protective influenza vaccine that is limited to a single cycle of replication, which induces a strong cross-reactive T cell response but a minimal Ab response to hemagglutinin after intranasal or aerosol administration. We tested whether an H3N2 S-FLU can protect pigs and ferrets from heterosubtypic H1N1 influenza challenge. Aerosol administration of S-FLU to pigs induced lung tissue-resident memory T cells and reduced lung pathology but not the viral load. In contrast, in ferrets, S-FLU reduced viral replication and aerosol transmission. Our data show that S-FLU has different protective efficacy in pigs and ferrets, and that in the absence of Ab, lung T cell immunity can reduce disease severity without reducing challenge viral replication.


PMID: 29703861 DOI: 10.4049/jimmunol.1800142
 
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