tetano
Editor, Senior Moderator
Vaccine. 2019 Nov 14. pii: S0264-410X(19)31513-0. doi: 10.1016/j.vaccine.2019.11.006. [Epub ahead of print] [h=1]Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challenge.[/h]
Sister?-Or? M[SUP]1[/SUP], Mart?nez-Pulgar?n S[SUP]2[/SUP], Solanes D[SUP]3[/SUP], Veljkovic V[SUP]4[/SUP], L?pez-Serrano S[SUP]5[/SUP], C?rdoba L[SUP]6[/SUP], Cord?n I[SUP]7[/SUP], Escribano JM[SUP]2[/SUP], Darji A[SUP]8[/SUP].
[h=3]Author information[/h] 1 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. 2 Alternative Gene Expression S.L. ALGENEX, Centro empresarial - Parque Cient?fico y Tecnol?gico Universidad Polit?cnica de Madrid Campus de Montegancedo, 28223 Pozuelo de Alarc?n, Spain. 3 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: david.solanes@irta.cat. 4 Biomed Protection, Galveston, 77550 TX, USA. 5 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: sergi.lopez@irta.cat. 6 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: lorena.cordoba@irta.cat. 7 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ivan.cordon@irta.cat. 8 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ayub.darji@irta.cat.
[h=3]Abstract[/h] The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing "flagellin-NG34/CS17 antigen" were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CS17 vaccine protected the vaccinated chickens and blocked viral shedding orally and cloacally. Furthermore, no apparent clinical signs were monitored in 10/12 vaccinated individuals. The mechanism of protection conferred is under investigation.
Copyright ? 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza; Baculovirus; Conserved peptides; Cross-protection; Flagellin; H7 subtype; Influenza vaccine; Pandemic flu
PMID: 31735501 DOI: 10.1016/j.vaccine.2019.11.006
Free full text
Sister?-Or? M[SUP]1[/SUP], Mart?nez-Pulgar?n S[SUP]2[/SUP], Solanes D[SUP]3[/SUP], Veljkovic V[SUP]4[/SUP], L?pez-Serrano S[SUP]5[/SUP], C?rdoba L[SUP]6[/SUP], Cord?n I[SUP]7[/SUP], Escribano JM[SUP]2[/SUP], Darji A[SUP]8[/SUP].
[h=3]Author information[/h] 1 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. 2 Alternative Gene Expression S.L. ALGENEX, Centro empresarial - Parque Cient?fico y Tecnol?gico Universidad Polit?cnica de Madrid Campus de Montegancedo, 28223 Pozuelo de Alarc?n, Spain. 3 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: david.solanes@irta.cat. 4 Biomed Protection, Galveston, 77550 TX, USA. 5 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: sergi.lopez@irta.cat. 6 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: lorena.cordoba@irta.cat. 7 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ivan.cordon@irta.cat. 8 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ayub.darji@irta.cat.
[h=3]Abstract[/h] The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing "flagellin-NG34/CS17 antigen" were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CS17 vaccine protected the vaccinated chickens and blocked viral shedding orally and cloacally. Furthermore, no apparent clinical signs were monitored in 10/12 vaccinated individuals. The mechanism of protection conferred is under investigation.
Copyright ? 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza; Baculovirus; Conserved peptides; Cross-protection; Flagellin; H7 subtype; Influenza vaccine; Pandemic flu
PMID: 31735501 DOI: 10.1016/j.vaccine.2019.11.006
Free full text