Mary Wilson
Well-known member
Yuanmei Zhu[SUP]1[/SUP],[SUP]*[/SUP], Danwei Yu[SUP]1[/SUP],[SUP]*[/SUP], Yang Han[SUP]2[/SUP],[SUP]*[/SUP], Hongxia Yan[SUP]1[/SUP], Huihui Chong[SUP]1[/SUP], Lili Ren[SUP]1[/SUP], Jianwei Wang[SUP]1[/SUP],[SUP]†[/SUP], Taisheng Li[SUP]2[/SUP],[SUP]†[/SUP] and Yuxian He[SUP]1[/SUP],[SUP]†[/SUP]
Science Advances 09 Oct 2020:
eabc9999
DOI: 10.1126/sciadv.abc9999
Abstract
The current COVID-19 pandemic is caused by SARS-CoV-2, a novel coronavirus genetically close to SARS-CoV, thus it is important to define the between antigenic cross-reactivity and neutralization. In this study, we first analyzed 20 convalescent serum samples collected from SARS-CoV infected individuals during the 2003 SARS outbreak. All patient sera reacted strongly with the S1 subunit and receptor-binding domain (RBD) of SARS-CoV, cross-reacted with the S ectodomain, S1, RBD, and S2 proteins of SARS-CoV-2, and neutralized both SARS-CoV and SARS-CoV-2 S protein-driven infections. Multiple panels of antisera from mice and rabbits immunized with a full-length S and RBD immunogens of SARS-CoV were also characterized, verifying the cross-reactive neutralization against SARS-CoV-2. Interestingly, we found that a palm civet SARS-CoV-derived RBD elicited more potent cross-neutralizing responses in immunized animals than the RBD from a human SARS-CoV strain, informing a strategy to develop universe vaccines against emerging CoV.
https://advances.sciencemag.org/con...SciMag&utm_source=JHubbard&utm_medium=Twitter
Science Advances 09 Oct 2020:
eabc9999
DOI: 10.1126/sciadv.abc9999
Abstract
The current COVID-19 pandemic is caused by SARS-CoV-2, a novel coronavirus genetically close to SARS-CoV, thus it is important to define the between antigenic cross-reactivity and neutralization. In this study, we first analyzed 20 convalescent serum samples collected from SARS-CoV infected individuals during the 2003 SARS outbreak. All patient sera reacted strongly with the S1 subunit and receptor-binding domain (RBD) of SARS-CoV, cross-reacted with the S ectodomain, S1, RBD, and S2 proteins of SARS-CoV-2, and neutralized both SARS-CoV and SARS-CoV-2 S protein-driven infections. Multiple panels of antisera from mice and rabbits immunized with a full-length S and RBD immunogens of SARS-CoV were also characterized, verifying the cross-reactive neutralization against SARS-CoV-2. Interestingly, we found that a palm civet SARS-CoV-derived RBD elicited more potent cross-neutralizing responses in immunized animals than the RBD from a human SARS-CoV strain, informing a strategy to develop universe vaccines against emerging CoV.
https://advances.sciencemag.org/con...SciMag&utm_source=JHubbard&utm_medium=Twitter