tetano
Editor, Senior Moderator
Nat Immunol. 2016 Aug 15. doi: 10.1038/ni.3533. [Epub ahead of print]
[h=1]Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.[/h] Ellebedy AH[SUP]1,[/SUP][SUP]2[/SUP], Jackson KJ[SUP]3[/SUP], Kissick HT[SUP]2,[/SUP][SUP]4[/SUP], Nakaya HI[SUP]5,[/SUP][SUP]6[/SUP], Davis CW[SUP]1,[/SUP][SUP]2[/SUP], Roskin KM[SUP]3[/SUP], McElroy AK[SUP]7,[/SUP][SUP]8[/SUP], Oshansky CM[SUP]9[/SUP], Elbein R[SUP]10[/SUP], Thomas S[SUP]10[/SUP], Lyon GM[SUP]10[/SUP], Spiropoulou CF[SUP]8[/SUP], Mehta AK[SUP]10[/SUP], Thomas PG[SUP]9[/SUP], Boyd SD[SUP]3[/SUP], Ahmed R[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.
PMID: 27525369 DOI: 10.1038/ni.3533
[PubMed - as supplied by publisher]
[h=1]Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination.[/h] Ellebedy AH[SUP]1,[/SUP][SUP]2[/SUP], Jackson KJ[SUP]3[/SUP], Kissick HT[SUP]2,[/SUP][SUP]4[/SUP], Nakaya HI[SUP]5,[/SUP][SUP]6[/SUP], Davis CW[SUP]1,[/SUP][SUP]2[/SUP], Roskin KM[SUP]3[/SUP], McElroy AK[SUP]7,[/SUP][SUP]8[/SUP], Oshansky CM[SUP]9[/SUP], Elbein R[SUP]10[/SUP], Thomas S[SUP]10[/SUP], Lyon GM[SUP]10[/SUP], Spiropoulou CF[SUP]8[/SUP], Mehta AK[SUP]10[/SUP], Thomas PG[SUP]9[/SUP], Boyd SD[SUP]3[/SUP], Ahmed R[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antigen-specific B cells bifurcate into antibody-secreting cells (ASCs) and memory B cells (MBCs) after infection or vaccination. ASCs (plasmablasts) have been extensively studied in humans, but less is known about B cells that become activated but do not differentiate into plasmablasts. Here we have defined the phenotype and transcriptional program of a subset of antigen-specific B cells, which we have called 'activated B cells' (ABCs), that were distinct from ASCs and were committed to the MBC lineage. We detected ABCs in humans after infection with Ebola virus or influenza virus and also after vaccination. By simultaneously analyzing antigen-specific ASCs and ABCs in human blood after vaccination against influenza virus, we investigated the clonal overlap and extent of somatic hypermutation (SHM) in the ASC (effector) and ABC (memory) lineages. Longitudinal tracking of vaccination-induced hemagglutinin (HA)-specific clones revealed no overall increase in SHM over time, which suggested that repeated annual immunization might have limitations in enhancing the quality of influenza-virus-specific antibody.
PMID: 27525369 DOI: 10.1038/ni.3533
[PubMed - as supplied by publisher]