tetano
Editor, Senior Moderator
Arch Virol. 2018 May 23. doi: 10.1007/s00705-018-3889-4. [Epub ahead of print]
[h=1]Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses.[/h] Yang F[SUP]1[/SUP], Chen B[SUP]1[/SUP], Liu F[SUP]1[/SUP], Peng X[SUP]1[/SUP], Sun T[SUP]1[/SUP], Yao H[SUP]1[/SUP], Wu H[SUP]2[/SUP], Wu N[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR primers were designed based on gene regions encoding conserved domains of the nucleoprotein and H10 hemagglutinin. The developed multiplex real-time RT-PCR assay displayed high specificity, repeatability, and a detection limit of 10 copies per reaction. This diagnostic method could prove valuable for the rapid detection of H10 subtype AIVs in China.
PMID: 29796926 DOI: 10.1007/s00705-018-3889-4
[h=1]Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses.[/h] Yang F[SUP]1[/SUP], Chen B[SUP]1[/SUP], Liu F[SUP]1[/SUP], Peng X[SUP]1[/SUP], Sun T[SUP]1[/SUP], Yao H[SUP]1[/SUP], Wu H[SUP]2[/SUP], Wu N[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR primers were designed based on gene regions encoding conserved domains of the nucleoprotein and H10 hemagglutinin. The developed multiplex real-time RT-PCR assay displayed high specificity, repeatability, and a detection limit of 10 copies per reaction. This diagnostic method could prove valuable for the rapid detection of H10 subtype AIVs in China.
PMID: 29796926 DOI: 10.1007/s00705-018-3889-4