Differential Oxidative Stress Induced by Dengue Virus in Monocytes from Human Neonates, Adult and Elderly Individuals
Nereida Valero,
Jes?s Mosquera mail,
Germ?n A?ez,
Alegria Levy,
Rafael Marcucci,
Melchor Alvarez de Mon
Published: September 17, 2013
DOI: 10.1371/journal.pone.007322
Abstract
Changes in immune response during lifespan of man are well known. These changes involve decreased neonatal and elderly immune response. In addition, it has been shown a relationship between immune and oxidative mechanisms, suggesting that altered immune response could be associated to altered oxidative response. Increased expression of nitric oxide (NO) has been documented in dengue and in monocyte cultures infected with different types of dengue virus. However, there is no information about the age-dependent NO oxidative response in humans infected by dengue virus. In this study, monocyte cultures from neonatal, elderly and adult individuals (n = 10 each group) were infected with different dengue virus types (DENV- 1 to 4) and oxidative/antioxidative responses and apoptosis were measured at days 1 and 3 of culture. Increased production of NO, lipid peroxidation and enzymatic and nonenzymatic anti-oxidative responses in dengue infected monocyte cultures were observed. However, neonatal and elderly monocytes had lower values of studied parameters when compared to those in adult-derived cultures. Apoptosis was present in infected monocytes with higher values at day 3 of culture. This reduced oxidant/antioxidant response of neonatal and elderly monocytes could be relevant in the pathogenesis of dengue disease.
Citation: Valero N, Mosquera J, A?ez G, Levy A, Marcucci R, et al. (2013) Differential Oxidative Stress Induced by Dengue Virus in Monocytes from Human Neonates, Adult and Elderly Individuals. PLoS ONE 8(9): e73221. doi:10.1371/journal.pone.0073221
Editor: Guangwei Liu, Department of Immunology, China
Received: May 3, 2013; Accepted: July 19, 2013; Published: September 17, 2013
Copyright: ? 2013 Valero et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from Fondo de Investigaci?n de la Seguridad Social (Spain), Consejer?a de Educaci?n, Comunidad de Madrid, MITIC-CM (S-2010/BMD-2502), Instituto de Salud Carlos III, MEC (PIO51871, CIBERehd) and CONDES CC-0393-12 (Maracaibo, Venezuela). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
