tetano
Editor, Senior Moderator
Biosens Bioelectron. 2017 Feb 16;92:234-240. doi: 10.1016/j.bios.2017.02.023. [Epub ahead of print]
[h=1]Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors.[/h] Horiguchi Y[SUP]1[/SUP], Goda T[SUP]1[/SUP], Matsumoto A[SUP]1[/SUP], Takeuchi H[SUP]2[/SUP], Yamaoka S[SUP]2[/SUP], Miyahara Y[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Biointerface; Biosensor; Influrenza virus; Label-free; Molecular recognition
PMID: 28222368 DOI: 10.1016/j.bios.2017.02.023
[PubMed - as supplied by publisher]
[h=1]Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors.[/h] Horiguchi Y[SUP]1[/SUP], Goda T[SUP]1[/SUP], Matsumoto A[SUP]1[/SUP], Takeuchi H[SUP]2[/SUP], Yamaoka S[SUP]2[/SUP], Miyahara Y[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Biointerface; Biosensor; Influrenza virus; Label-free; Molecular recognition
PMID: 28222368 DOI: 10.1016/j.bios.2017.02.023
[PubMed - as supplied by publisher]