tetano
Editor, Senior Moderator
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2013 Mar;35(1):24-8. doi: 10.3881/j.issn.1000-503X.2013.01.005.
Establishment of a High-throughput Respiratory Viruse DetectionTechnology without RNA Purification and Reverse Transcription.
Dan-Li Y, Xiao-Yi T, Wei-Xian S, Zhi Z.
Source
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Abstract
Objective To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring. Methods We used high-throughput microsphere-based flexible multi-analyte profiling techndogy (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated. Results There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies. Conclusion We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.
PMID:
23469786
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23469786
Establishment of a High-throughput Respiratory Viruse DetectionTechnology without RNA Purification and Reverse Transcription.
Dan-Li Y, Xiao-Yi T, Wei-Xian S, Zhi Z.
Source
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Abstract
Objective To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring. Methods We used high-throughput microsphere-based flexible multi-analyte profiling techndogy (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated. Results There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies. Conclusion We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.
PMID:
23469786
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/23469786