tetano
Editor, Senior Moderator
J Appl Microbiol. 2012 Apr 30. doi: 10.1111/j.1365-2672.2012.05326.x. [Epub ahead of print]
Evaluation of Leuconostoc mesenteroides YML003 as a probiotic against Low-Pathogenic Avian Influenza (H9N2) Virus in chickens.
Seo BJ, Ahmad RI, Rejish Kumar VJ, Choi UH, Moon MR, Lim J, Park YH.
Source
Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyongsan-si, Gyongsangbuk-Do 712-749, Republic of Korea.
Abstract
Aim: This study was performed to isolate anti- H9N2 bacteria from Korean Kimchi isolates and to evaluate its performance in cell line, egg and in specific pathogen free (SPF) chickens. Methods and Results: Using Madin-Darby canine kidney (MDCK) cell line 220 bacterial isolates were screened and the isolate YML003 was selected having pronounced antiviral activity against H9N2 virus. This isolate was identified as Leuconostoc mesenteroides by 16S rRNA gene sequencing. Anti H9N2 activity of the strain was also evaluated by hemagglutination assay. Leuconostoc mesenteroides YML003 was assessed for its survival in gastric juice and 5% bile acid and the antibiotic sucessptability. Both live and heat killed cells were selected for in vivo chicken feeding experiment. Body weight, immune index, serobiochemical parameters and splenic IFN-γ production were assessed during selected intervals. Viral population in the trachea and cloacae were calculated by quantitative real time reverse transcriptase PCR (qRT-PCR). Conclusions: Leuconostoc mesenteroides YML003 exhibited anti H9N2 activity both in in vitro cell line as well as in vivo SPF Chickens. Significance and Impact of the study: This is a primary report on the anti-H9N2 activity by a Leuconostoc strain. Amid the increasing reports of avian influenza virus occurrence resulting in severe losses to the poultry industry, prophylactic administration of such probiotic strains are highly significant. ? 2012The Authors Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.
? 2012The Authors Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.
PMID:
22548634
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22548634
Evaluation of Leuconostoc mesenteroides YML003 as a probiotic against Low-Pathogenic Avian Influenza (H9N2) Virus in chickens.
Seo BJ, Ahmad RI, Rejish Kumar VJ, Choi UH, Moon MR, Lim J, Park YH.
Source
Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyongsan-si, Gyongsangbuk-Do 712-749, Republic of Korea.
Abstract
Aim: This study was performed to isolate anti- H9N2 bacteria from Korean Kimchi isolates and to evaluate its performance in cell line, egg and in specific pathogen free (SPF) chickens. Methods and Results: Using Madin-Darby canine kidney (MDCK) cell line 220 bacterial isolates were screened and the isolate YML003 was selected having pronounced antiviral activity against H9N2 virus. This isolate was identified as Leuconostoc mesenteroides by 16S rRNA gene sequencing. Anti H9N2 activity of the strain was also evaluated by hemagglutination assay. Leuconostoc mesenteroides YML003 was assessed for its survival in gastric juice and 5% bile acid and the antibiotic sucessptability. Both live and heat killed cells were selected for in vivo chicken feeding experiment. Body weight, immune index, serobiochemical parameters and splenic IFN-γ production were assessed during selected intervals. Viral population in the trachea and cloacae were calculated by quantitative real time reverse transcriptase PCR (qRT-PCR). Conclusions: Leuconostoc mesenteroides YML003 exhibited anti H9N2 activity both in in vitro cell line as well as in vivo SPF Chickens. Significance and Impact of the study: This is a primary report on the anti-H9N2 activity by a Leuconostoc strain. Amid the increasing reports of avian influenza virus occurrence resulting in severe losses to the poultry industry, prophylactic administration of such probiotic strains are highly significant. ? 2012The Authors Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.
? 2012The Authors Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.
PMID:
22548634
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22548634