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Evaluation of two workflows for whole genome sequencing-based typing of influenza A viruses

tetano

Editor, Senior Moderator
J Virol Methods. 2019 Jan 21. pii: S0166-0934(18)30496-8. doi: 10.1016/j.jviromet.2019.01.009. [Epub ahead of print]
[h=1]Evaluation of two workflows for whole genome sequencing-based typing of influenza A viruses.[/h] W?thrich D[SUP]1[/SUP], Lang D[SUP]2[/SUP], M?ller NF[SUP]3[/SUP], Neher RA[SUP]4[/SUP], Stadler T[SUP]3[/SUP], Egli A[SUP]5[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] We compared two sample preparation protocols for whole genome sequencing of influenza A viruses. Each protocol was assessed using cDNA quantity and quality and the resulting mean genome coverage after sequencing. Both protocols produced acceptable result for samples with high viral load, whereas one protocol performed slightly better with limited virus count.
Copyright ? 2019. Published by Elsevier B.V.


[h=4]KEYWORDS:[/h] Whole Genome Sequencing; influenza A; method; typing; virus

PMID: 30677464 DOI: 10.1016/j.jviromet.2019.01.009
 
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