tetano
Editor, Senior Moderator
Am J Respir Cell Mol Biol. 2013 Oct 17. [Epub ahead of print]
Evidence for Scgb1a1+ Cells in the Generation of p63+ Cells in the Damaged Lung Parenchyma.
Zheng D, Yin L, Chen J.
Source
Singapore-MIT Alliance for Research and Technology, Interdisciplinary Research Group in Infectious Diseases, Singapore, Singapore ; zhengdahai@smart.mit.edu.
Abstract
Transformation related protein 63-expressing (p63+) basal cells are confined to the trachea in the mouse lung. However, after influenza virus infection or bleomycin treatment, patches of p63+ cells were observed in the damaged lung parenchyma. To address whether the newly induced p63+ cells are derived from the p63+ basal cells, we carried out lineage tracing. In keratin 5 (Krt5) promoter-driven CreER system, although pre-existing p63+ basal cells were labeled by EGFP following tamoxifen treatment, none or only a small fraction (~15%) of the p63+ patches were labeled by EGFP following bleomycin treatment or influenza virus infection, respectively. In contrast, most (>60%) of p63+ patches contained EGFP+ cells in Scgb1a1-CreER transgenic system where Clara cells are labeled. Furthermore, many p63+ cells were found in bronchiole-like lumen structures with columnar cells at the lumen side. The columnar cells were positive for Clara cell marker Cyp2f2 and could be traced to the newly induced p63+ cells. These results suggest that most of the newly induced p63+ cells in the damaged parenchyma are likely derived from Clara cells rather than p63+ basal cells and that newly induced p63+ cells may be involved in the regeneration of bronchioles.
PMID:
24134540
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24134540
Evidence for Scgb1a1+ Cells in the Generation of p63+ Cells in the Damaged Lung Parenchyma.
Zheng D, Yin L, Chen J.
Source
Singapore-MIT Alliance for Research and Technology, Interdisciplinary Research Group in Infectious Diseases, Singapore, Singapore ; zhengdahai@smart.mit.edu.
Abstract
Transformation related protein 63-expressing (p63+) basal cells are confined to the trachea in the mouse lung. However, after influenza virus infection or bleomycin treatment, patches of p63+ cells were observed in the damaged lung parenchyma. To address whether the newly induced p63+ cells are derived from the p63+ basal cells, we carried out lineage tracing. In keratin 5 (Krt5) promoter-driven CreER system, although pre-existing p63+ basal cells were labeled by EGFP following tamoxifen treatment, none or only a small fraction (~15%) of the p63+ patches were labeled by EGFP following bleomycin treatment or influenza virus infection, respectively. In contrast, most (>60%) of p63+ patches contained EGFP+ cells in Scgb1a1-CreER transgenic system where Clara cells are labeled. Furthermore, many p63+ cells were found in bronchiole-like lumen structures with columnar cells at the lumen side. The columnar cells were positive for Clara cell marker Cyp2f2 and could be traced to the newly induced p63+ cells. These results suggest that most of the newly induced p63+ cells in the damaged parenchyma are likely derived from Clara cells rather than p63+ basal cells and that newly induced p63+ cells may be involved in the regeneration of bronchioles.
PMID:
24134540
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24134540