tetano
Editor, Senior Moderator
Lett Appl Microbiol. 2017 Apr 2. doi: 10.1111/lam.12738. [Epub ahead of print]
[h=1]Expression of surface-bound non-structural 1 (NS1) protein of influenza virus A H5N1 on Lactobacillus casei strain C1.[/h] Tan TS[SUP]1[/SUP], Syed Hassan S[SUP]2[/SUP], Yap WB[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The study aimed to construct a recombinant Lactobacillus casei (L. casei) expressing the non-structural (NS) 1 protein of influenza A virus H5N1 on its cell wall. The NS1 gene was first amplified and fused to the pSGANC332 expression plasmid. The NS1 protein expression was carried out by L. casei strain C1. PCR screening and DNA sequencing confirmed the presence of recombinant pSG-NS1-ANC332 plasmid in L. casei. The plasmid was stably maintained (98.94?1.65%) by the bacterium within the first 20 generations without selective pressure. The NS1 was expressed as a 49-kDa protein in association with the anchoring peptide. The yield was 1.325?0.065 μg per mg of bacterial cells. L. casei expressing the NS1 on its cell wall was red-fluorescently stained but the staining was not observed on L. casei carrying the empty pSGANC332. The results implied that L. casei strain C1 is a promising host for the expression of surface-bound NS1 protein by using the pSGANC332 expression plasmid. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Lactobacillus casei ; Cloning; NS1; influenza viruses; pSGANC332; protein expression
PMID: 28370088 DOI: 10.1111/lam.12738
[h=1]Expression of surface-bound non-structural 1 (NS1) protein of influenza virus A H5N1 on Lactobacillus casei strain C1.[/h] Tan TS[SUP]1[/SUP], Syed Hassan S[SUP]2[/SUP], Yap WB[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The study aimed to construct a recombinant Lactobacillus casei (L. casei) expressing the non-structural (NS) 1 protein of influenza A virus H5N1 on its cell wall. The NS1 gene was first amplified and fused to the pSGANC332 expression plasmid. The NS1 protein expression was carried out by L. casei strain C1. PCR screening and DNA sequencing confirmed the presence of recombinant pSG-NS1-ANC332 plasmid in L. casei. The plasmid was stably maintained (98.94?1.65%) by the bacterium within the first 20 generations without selective pressure. The NS1 was expressed as a 49-kDa protein in association with the anchoring peptide. The yield was 1.325?0.065 μg per mg of bacterial cells. L. casei expressing the NS1 on its cell wall was red-fluorescently stained but the staining was not observed on L. casei carrying the empty pSGANC332. The results implied that L. casei strain C1 is a promising host for the expression of surface-bound NS1 protein by using the pSGANC332 expression plasmid. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Lactobacillus casei ; Cloning; NS1; influenza viruses; pSGANC332; protein expression
PMID: 28370088 DOI: 10.1111/lam.12738