tetano
Editor, Senior Moderator
Front Immunol
. 2022 Sep 7;13:889836.
doi: 10.3389/fimmu.2022.889836. eCollection 2022.
Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity
Julius Nückel[SUP] 1 2 [/SUP], Elisa Planatscher[SUP] 1 [/SUP], Anne Wiebe Mohr[SUP] 2 3 [/SUP], Karolin Deichl[SUP] 1 [/SUP], Hrvoje Mijočević[SUP] 4 [/SUP], Martin Feuerherd[SUP] 4 [/SUP], Lisa Wolff[SUP] 4 [/SUP], Johanna Erber[SUP] 5 [/SUP], Jochen Schneider[SUP] 5 [/SUP], Michael Quante[SUP] 5 [/SUP], Christoph Winter[SUP] 6 7 [/SUP], Jürgen Ruland[SUP] 6 7 2 [/SUP], Alexander Hapfelmeier[SUP] 8 [/SUP], Wolfgang Hammerschmidt[SUP] 9 [/SUP], Andreas Moosmann[SUP] 2 3 [/SUP], Ulrike Protzer[SUP] 2 4 [/SUP], Uta Behrends[SUP] 1 2 4 [/SUP], Josef Mautner[SUP] 1 2 4 9 [/SUP]
Affiliations
Abstract
Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.
Keywords: COVID-19; SARS-CoV-2; coronavirus; multiplex serology; nucleocapsid protein.
. 2022 Sep 7;13:889836.
doi: 10.3389/fimmu.2022.889836. eCollection 2022.
Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity
Julius Nückel[SUP] 1 2 [/SUP], Elisa Planatscher[SUP] 1 [/SUP], Anne Wiebe Mohr[SUP] 2 3 [/SUP], Karolin Deichl[SUP] 1 [/SUP], Hrvoje Mijočević[SUP] 4 [/SUP], Martin Feuerherd[SUP] 4 [/SUP], Lisa Wolff[SUP] 4 [/SUP], Johanna Erber[SUP] 5 [/SUP], Jochen Schneider[SUP] 5 [/SUP], Michael Quante[SUP] 5 [/SUP], Christoph Winter[SUP] 6 7 [/SUP], Jürgen Ruland[SUP] 6 7 2 [/SUP], Alexander Hapfelmeier[SUP] 8 [/SUP], Wolfgang Hammerschmidt[SUP] 9 [/SUP], Andreas Moosmann[SUP] 2 3 [/SUP], Ulrike Protzer[SUP] 2 4 [/SUP], Uta Behrends[SUP] 1 2 4 [/SUP], Josef Mautner[SUP] 1 2 4 9 [/SUP]
Affiliations
- PMID: 36159804
- PMCID: PMC9490404
- DOI: 10.3389/fimmu.2022.889836
Abstract
Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.
Keywords: COVID-19; SARS-CoV-2; coronavirus; multiplex serology; nucleocapsid protein.