tetano
Editor, Senior Moderator
Gene Rep
. 2021 Jun;23:101045.
doi: 10.1016/j.genrep.2021.101045. Epub 2021 Feb 16.
SARS-CoV-2 spillover transmission due to recombination event
Nariman Shahhosseini[SUP] 1 [/SUP], Gary Wong[SUP] 1 2 [/SUP], Gary P Kobinger[SUP] 1 3 4 5 [/SUP], Sadegh Chinikar[SUP] 6 7 [/SUP]
Affiliations
Abstract
In late 2019, a novel Coronavirus emerged in China. Perceiving the modulating factors of cross-species virus transmission is critical to elucidate the nature of virus emergence. Using bioinformatics tools, we analyzed the mapping of the SARS-CoV-2 genome, modeling of protein structure, and analyze the evolutionary origin of SARS-CoV-2, as well as potential recombination events. Phylogenetic tree analysis shows that SARS-CoV-2 has the closest evolutionary relationship with Bat-SL-CoV-2 (RaTG13) at the scale of the complete virus genome, and less similarity to Pangolin-CoV. However, the Receptor Binding Domain (RBD) of SARS-CoV-2 is almost identical to Pangolin-CoV at the aa level, suggesting that spillover transmission probably occurred directly from pangolins, but not bats. Further recombination analysis revealed the pathway for spillover transmission from Bat-SL-CoV-2 and Pangolin-CoV. Here, we provide evidence for recombination event between Bat-SL-CoV-2 and Pangolin-CoV that resulted in the emergence of SARS-CoV-2. Nevertheless, the role of mutations should be noted as another influencing factor in the continuing evolution and resurgence of novel SARS-CoV-2 variants.
Keywords: Bat-SL-CoV-2, Bat SARS like Coronavirus 2; COVID-19, coronavirus disease 2019; CoV, coronavirus; MERS, Middle East Respiratory Syndrome; Mutation; Pandemic; Phylogenetics; RBD, receptor binding domain; Recombination; SARS, severe acute respiratory syndrome; SARS-CoV-2; Virulence; hACE2, human angiotensin-converting enzyme 2.
. 2021 Jun;23:101045.
doi: 10.1016/j.genrep.2021.101045. Epub 2021 Feb 16.
SARS-CoV-2 spillover transmission due to recombination event
Nariman Shahhosseini[SUP] 1 [/SUP], Gary Wong[SUP] 1 2 [/SUP], Gary P Kobinger[SUP] 1 3 4 5 [/SUP], Sadegh Chinikar[SUP] 6 7 [/SUP]
Affiliations
- PMID: 33615041
- PMCID: PMC7884226
- DOI: 10.1016/j.genrep.2021.101045
Abstract
In late 2019, a novel Coronavirus emerged in China. Perceiving the modulating factors of cross-species virus transmission is critical to elucidate the nature of virus emergence. Using bioinformatics tools, we analyzed the mapping of the SARS-CoV-2 genome, modeling of protein structure, and analyze the evolutionary origin of SARS-CoV-2, as well as potential recombination events. Phylogenetic tree analysis shows that SARS-CoV-2 has the closest evolutionary relationship with Bat-SL-CoV-2 (RaTG13) at the scale of the complete virus genome, and less similarity to Pangolin-CoV. However, the Receptor Binding Domain (RBD) of SARS-CoV-2 is almost identical to Pangolin-CoV at the aa level, suggesting that spillover transmission probably occurred directly from pangolins, but not bats. Further recombination analysis revealed the pathway for spillover transmission from Bat-SL-CoV-2 and Pangolin-CoV. Here, we provide evidence for recombination event between Bat-SL-CoV-2 and Pangolin-CoV that resulted in the emergence of SARS-CoV-2. Nevertheless, the role of mutations should be noted as another influencing factor in the continuing evolution and resurgence of novel SARS-CoV-2 variants.
Keywords: Bat-SL-CoV-2, Bat SARS like Coronavirus 2; COVID-19, coronavirus disease 2019; CoV, coronavirus; MERS, Middle East Respiratory Syndrome; Mutation; Pandemic; Phylogenetics; RBD, receptor binding domain; Recombination; SARS, severe acute respiratory syndrome; SARS-CoV-2; Virulence; hACE2, human angiotensin-converting enzyme 2.