tetano
Editor, Senior Moderator
Arch Virol. 2018 Jan 6. doi: 10.1007/s00705-017-3695-4. [Epub ahead of print]
[h=1]Genetic and biological characterization of three poultry-origin H5N6 avian influenza viruses with all internal genes from genotype S H9N2 viruses.[/h] Liu K[SUP]1[/SUP], Gu M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Hu S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Gao R[SUP]1[/SUP], Li J[SUP]1[/SUP], Shi L[SUP]1[/SUP], Sun W[SUP]1[/SUP], Liu D[SUP]1[/SUP], Gao Z[SUP]1[/SUP], Xu X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Hu J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Wang X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]1,[/SUP][SUP]3[/SUP], Chen S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Peng D[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Jiao X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]4,[/SUP][SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] During surveillance for avian influenza viruses, three H5N6 viruses were isolated in chickens obtained from live bird markets in eastern China, between January 2015 and April 2016. Sequence analysis revealed a high genomic homology between these poultry isolates and recent human H5N6 variants whose internal genes were derived from genotype S H9N2 avian influenza viruses. Glycan binding assays revealed that all avian H5N6 viruses were capable of binding to both human-type SAα-2,6Gal receptors and avian-type SAα-2,3Gal receptors. Their biological characteristics were further studied in BALB/c mice, specific-pathogen-free chickens, and mallard ducks. All three isolates had low pathogenicity in mice but were highly pathogenic to chickens, as evidenced by 100% mortality 36-120 hours post infection at a low dose of 10[SUP]3.0[/SUP]EID[SUB]50[/SUB] and through effective contact transmission. Moreover, all three poultry H5N6 isolates caused asymptomatic infections in ducks, which may serve as a reservoir host for their maintenance and dissemination; these migrating waterfowl could cause a potential global pandemic. Our study suggests that continuous epidemiological surveillance in poultry should be implemented for the early prevention of future influenza outbreaks.
PMID: 29307089 DOI: 10.1007/s00705-017-3695-4
[h=1]Genetic and biological characterization of three poultry-origin H5N6 avian influenza viruses with all internal genes from genotype S H9N2 viruses.[/h] Liu K[SUP]1[/SUP], Gu M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Hu S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Gao R[SUP]1[/SUP], Li J[SUP]1[/SUP], Shi L[SUP]1[/SUP], Sun W[SUP]1[/SUP], Liu D[SUP]1[/SUP], Gao Z[SUP]1[/SUP], Xu X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Hu J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Wang X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]1,[/SUP][SUP]3[/SUP], Chen S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Peng D[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Jiao X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Liu X[SUP]4,[/SUP][SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] During surveillance for avian influenza viruses, three H5N6 viruses were isolated in chickens obtained from live bird markets in eastern China, between January 2015 and April 2016. Sequence analysis revealed a high genomic homology between these poultry isolates and recent human H5N6 variants whose internal genes were derived from genotype S H9N2 avian influenza viruses. Glycan binding assays revealed that all avian H5N6 viruses were capable of binding to both human-type SAα-2,6Gal receptors and avian-type SAα-2,3Gal receptors. Their biological characteristics were further studied in BALB/c mice, specific-pathogen-free chickens, and mallard ducks. All three isolates had low pathogenicity in mice but were highly pathogenic to chickens, as evidenced by 100% mortality 36-120 hours post infection at a low dose of 10[SUP]3.0[/SUP]EID[SUB]50[/SUB] and through effective contact transmission. Moreover, all three poultry H5N6 isolates caused asymptomatic infections in ducks, which may serve as a reservoir host for their maintenance and dissemination; these migrating waterfowl could cause a potential global pandemic. Our study suggests that continuous epidemiological surveillance in poultry should be implemented for the early prevention of future influenza outbreaks.
PMID: 29307089 DOI: 10.1007/s00705-017-3695-4