tetano
Editor, Senior Moderator
PLoS One. 2019 Apr 25;14(4):e0216247. doi: 10.1371/journal.pone.0216247. eCollection 2019.
[h=1]Highly sensitive detection of influenza virus with SERS aptasensor.[/h] Kukushkin VI[SUP]1[/SUP], Ivanov NM[SUP]2[/SUP], Novoseltseva AA[SUP]2[/SUP], Gambaryan AS[SUP]3[/SUP], Yaminsky IV[SUP]2[/SUP], Kopylov AM[SUP]2[/SUP], Zavyalova EG[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Highly sensitive and rapid technology of surface enhanced Raman scattering (SERS) was applied to create aptasensors for influenza virus detection. SERS achieves 106-109 times signal amplification, yielding excellent sensitivity, whereas aptamers to hemagglutinin provide a specific recognition of the influenza virus. Aptamer RHA0385 was demonstrated to have essentially broad strain-specificity toward both recombinant hemagglutinins and the whole viruses. To achieve high sensitivity, a sandwich of primary aptamers, influenza virus and secondary aptamers was assembled. Primary aptamers were attached to metal particles of a SERS substrate, and influenza viruses were captured and bound with secondary aptamers labelled with Raman-active molecules. The signal was affected by the concentration of both primary and secondary aptamers. The limit of detection was as low as 1 ? 10-4 hemagglutination units per probe as tested for the H3N2 virus (A/England/42/72). Aptamer-based sensors provided recognition of various influenza viral strains, including H1, H3, and H5 hemagglutinin subtypes. Therefore, the aptasensors could be applied for fast and low-cost strain-independent determination of influenza viruses.
PMID: 31022287 DOI: 10.1371/journal.pone.0216247
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[h=1]Highly sensitive detection of influenza virus with SERS aptasensor.[/h] Kukushkin VI[SUP]1[/SUP], Ivanov NM[SUP]2[/SUP], Novoseltseva AA[SUP]2[/SUP], Gambaryan AS[SUP]3[/SUP], Yaminsky IV[SUP]2[/SUP], Kopylov AM[SUP]2[/SUP], Zavyalova EG[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Highly sensitive and rapid technology of surface enhanced Raman scattering (SERS) was applied to create aptasensors for influenza virus detection. SERS achieves 106-109 times signal amplification, yielding excellent sensitivity, whereas aptamers to hemagglutinin provide a specific recognition of the influenza virus. Aptamer RHA0385 was demonstrated to have essentially broad strain-specificity toward both recombinant hemagglutinins and the whole viruses. To achieve high sensitivity, a sandwich of primary aptamers, influenza virus and secondary aptamers was assembled. Primary aptamers were attached to metal particles of a SERS substrate, and influenza viruses were captured and bound with secondary aptamers labelled with Raman-active molecules. The signal was affected by the concentration of both primary and secondary aptamers. The limit of detection was as low as 1 ? 10-4 hemagglutination units per probe as tested for the H3N2 virus (A/England/42/72). Aptamer-based sensors provided recognition of various influenza viral strains, including H1, H3, and H5 hemagglutinin subtypes. Therefore, the aptasensors could be applied for fast and low-cost strain-independent determination of influenza viruses.
PMID: 31022287 DOI: 10.1371/journal.pone.0216247
Free full text