Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells

[tetano]

Nan Fang Yi Ke Da Xue Xue Bao. 2013 Feb;33(2):282-6.
[Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells].
[Article in Chinese]
Tang BK, Wu SW, Hu MJ, Liu XF.
Source

Department of Life Science, Bengbu Medical College, Bengbu 233030, China. E-mail: bktang.bbmc@gmail.com.
Abstract
OBJECTIVE:

To investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells.
METHODS:

A549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively.
RESULTS:

Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression.
CONCLUSIONS:

Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation.

PMID:
23443790
[PubMed - in process]

Free full text

http://www.ncbi.nlm.nih.gov/pubmed/23443790