tetano
Editor, Senior Moderator
J Gen Virol. 2014 Sep 18. pii: vir.0.068585-0. doi: 10.1099/vir.0.068585-0. [Epub ahead of print]
Human microRNA-24 modulates highly pathogenic avian-origin H5N1 influenza A virus infection in A549 cells by targeting secretory pathway furin.
Loveday EK1, Diederich S1, Pasick J2, Jean F3.
Author information
Abstract
A common critical cellular event many human enveloped viruses share is the requirement for proteolytic cleavage of the viral glycoprotein by furin in the host secretory pathway. For example, the furin-dependent proteolytic activation of highly pathogenic (HP) influenza A (infA) H5 and H7 hemagglutinin precursor (HA0) subtypes is critical for yielding fusion-competent infectious virions. In this study, we hypothesize that viral hijacking of the furin pathway by HP infA viruses to permit cleavage of HA0 could represent a novel molecular mechanism controlling the dynamic production of fusion-competent infectious virus particles during the viral lifecycle. We explore the biological role in this process of a newly identified furin-directed human microRNA, miR-24, as a potential post-transcriptional regulator of the furin-mediated activation of HA0 and production of fusion-competent virions in the host secretory pathway. We report that miR-24 and furin are differentially expressed in human A549 cells infected with HP avian-origin infA H5N1. Using miR-24 mimics, we demonstrate a robust decrease in both furin mRNA level and intracellular furin activity in A549 cells. Importantly, pretreatment of A549 cells with miR-24 mimics results in a robust decrease in the production of H5N1 infectious virions and a complete block of H5N1 virus spread that was not observed in A549 cells infected with low-pathogenicity swine-origin infA H1N1 virus. Our results suggest that viral-specific down-regulation of furin-directed microRNAs such as miR-24 during the lifecycle of HP infA viruses may represent a novel regulatory mechanism that governs furin-mediated proteolytic activation of HA0 glycoproteins and production of infectious virions.
Copyright ? 2014, the Society for General Microbiology.
KEYWORDS:
H5N1 influenza A virus; Proprotein convertases; furin; highly pathogenic avian-origin influenza A virus; human microRNA
PMID:
25234642
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25234642
Human microRNA-24 modulates highly pathogenic avian-origin H5N1 influenza A virus infection in A549 cells by targeting secretory pathway furin.
Loveday EK1, Diederich S1, Pasick J2, Jean F3.
Author information
Abstract
A common critical cellular event many human enveloped viruses share is the requirement for proteolytic cleavage of the viral glycoprotein by furin in the host secretory pathway. For example, the furin-dependent proteolytic activation of highly pathogenic (HP) influenza A (infA) H5 and H7 hemagglutinin precursor (HA0) subtypes is critical for yielding fusion-competent infectious virions. In this study, we hypothesize that viral hijacking of the furin pathway by HP infA viruses to permit cleavage of HA0 could represent a novel molecular mechanism controlling the dynamic production of fusion-competent infectious virus particles during the viral lifecycle. We explore the biological role in this process of a newly identified furin-directed human microRNA, miR-24, as a potential post-transcriptional regulator of the furin-mediated activation of HA0 and production of fusion-competent virions in the host secretory pathway. We report that miR-24 and furin are differentially expressed in human A549 cells infected with HP avian-origin infA H5N1. Using miR-24 mimics, we demonstrate a robust decrease in both furin mRNA level and intracellular furin activity in A549 cells. Importantly, pretreatment of A549 cells with miR-24 mimics results in a robust decrease in the production of H5N1 infectious virions and a complete block of H5N1 virus spread that was not observed in A549 cells infected with low-pathogenicity swine-origin infA H1N1 virus. Our results suggest that viral-specific down-regulation of furin-directed microRNAs such as miR-24 during the lifecycle of HP infA viruses may represent a novel regulatory mechanism that governs furin-mediated proteolytic activation of HA0 glycoproteins and production of infectious virions.
Copyright ? 2014, the Society for General Microbiology.
KEYWORDS:
H5N1 influenza A virus; Proprotein convertases; furin; highly pathogenic avian-origin influenza A virus; human microRNA
PMID:
25234642
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25234642