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Editor, Senior Moderator
Respir Res. 2019 Aug 15;20(1):184. doi: 10.1186/s12931-019-1153-4.
[h=1]IL-22Ra1 is induced during influenza infection by direct and indirect TLR3 induction of STAT1.[/h] Hebert KD[SUP]1[/SUP], Mclaughlin N[SUP]1[/SUP], Zhang Z[SUP]1[/SUP], Cipriani A[SUP]2[/SUP], Alcorn JF[SUP]2[/SUP], Pociask DA[SUP]3[/SUP].
[h=3]Author information[/h] 1 Department of Medicine, Pulmonary Diseases, Critical Care & Environmental Medicine, Tulane University School of Medicine, 1430 Tulane Ave. Mail code sl279, New Orleans, LA, 70112, USA. 2 Department of Pediatrics, Children's Hospital of Pittsburgh of UPMC, One Children's Hospital Drive, 4401 Penn Ave, Pittsburgh, PA, 15224, USA. 3 Department of Medicine, Pulmonary Diseases, Critical Care & Environmental Medicine, Tulane University School of Medicine, 1430 Tulane Ave. Mail code sl279, New Orleans, LA, 70112, USA. dpociask@tulane.edu.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza attacks the epithelium of the lung, causing cell death and disruption of the epithelial barrier leading to fluid buildup in the lung and impairment of gas exchange. Limited treatment options for severe influenza pneumonia prioritize the need for the discovery of effective therapies. IL-22 is a cytokine that promotes tissue integrity and has strong promise as a treatment option. While research has been focused on the cytokine itself, there is limited understanding of the regulation of the IL-22 receptor (IL-22Ra1) at the epithelial surface during infection.
[h=4]METHODS:[/h] IL-22Ra1 levels were measured by qRT-PCR, western blot and immunofluorescence following H1N1 influenza infection (A/PR/8/34 H1N1) or synthetic TLR3 mimetic, Poly (I:C). Regulation of the receptor was determined using STAT inhibitors (STAT1, STAT3 and PanSTAT inhibitors), TLR3 inhibition, and neutralization of interferon alpha receptor 2 (IFNAR2). Significance was determined by a p-value of greater than 0.05. Significance between two groups was measured using unpaired t-test and significance between more than two groups was measured using one-way ANOVA with Tukey Multiple Comparison Test.
[h=4]RESULTS:[/h] Here we show both in vivo and in vitro that IL-22Ra1 was induced as early as 24 h after influenza (H1N1 PR8) infection. This induction was triggered by toll-like receptor 3 (TLR3) as a TLR3 mimetic [Poly (I:C)] also induced IL-22Ra1 and inhibition of endosomal formation required for TLR3 function inhibited this process. This upregulation was dependent upon IFNβ signaling through STAT1. Importantly, induction of IL-22Ra1 significantly increased IL-22 signaling as evidenced by pSTAT3 levels following IL-22 treatment.
[h=4]CONCLUSION:[/h] Collectively, these data suggest epithelial cells may optimize the beneficial effects of IL-22 through the induction of the IL-22 receptor during viral infection in the lung.
[h=4]KEYWORDS:[/h] IFNβ; IL-22; IL-22Ra1; Influenza; STAT1; TLR3
PMID: 31416461 PMCID: PMC6694528 DOI: 10.1186/s12931-019-1153-4
Free PMC Article
[h=1]IL-22Ra1 is induced during influenza infection by direct and indirect TLR3 induction of STAT1.[/h] Hebert KD[SUP]1[/SUP], Mclaughlin N[SUP]1[/SUP], Zhang Z[SUP]1[/SUP], Cipriani A[SUP]2[/SUP], Alcorn JF[SUP]2[/SUP], Pociask DA[SUP]3[/SUP].
[h=3]Author information[/h] 1 Department of Medicine, Pulmonary Diseases, Critical Care & Environmental Medicine, Tulane University School of Medicine, 1430 Tulane Ave. Mail code sl279, New Orleans, LA, 70112, USA. 2 Department of Pediatrics, Children's Hospital of Pittsburgh of UPMC, One Children's Hospital Drive, 4401 Penn Ave, Pittsburgh, PA, 15224, USA. 3 Department of Medicine, Pulmonary Diseases, Critical Care & Environmental Medicine, Tulane University School of Medicine, 1430 Tulane Ave. Mail code sl279, New Orleans, LA, 70112, USA. dpociask@tulane.edu.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza attacks the epithelium of the lung, causing cell death and disruption of the epithelial barrier leading to fluid buildup in the lung and impairment of gas exchange. Limited treatment options for severe influenza pneumonia prioritize the need for the discovery of effective therapies. IL-22 is a cytokine that promotes tissue integrity and has strong promise as a treatment option. While research has been focused on the cytokine itself, there is limited understanding of the regulation of the IL-22 receptor (IL-22Ra1) at the epithelial surface during infection.
[h=4]METHODS:[/h] IL-22Ra1 levels were measured by qRT-PCR, western blot and immunofluorescence following H1N1 influenza infection (A/PR/8/34 H1N1) or synthetic TLR3 mimetic, Poly (I:C). Regulation of the receptor was determined using STAT inhibitors (STAT1, STAT3 and PanSTAT inhibitors), TLR3 inhibition, and neutralization of interferon alpha receptor 2 (IFNAR2). Significance was determined by a p-value of greater than 0.05. Significance between two groups was measured using unpaired t-test and significance between more than two groups was measured using one-way ANOVA with Tukey Multiple Comparison Test.
[h=4]RESULTS:[/h] Here we show both in vivo and in vitro that IL-22Ra1 was induced as early as 24 h after influenza (H1N1 PR8) infection. This induction was triggered by toll-like receptor 3 (TLR3) as a TLR3 mimetic [Poly (I:C)] also induced IL-22Ra1 and inhibition of endosomal formation required for TLR3 function inhibited this process. This upregulation was dependent upon IFNβ signaling through STAT1. Importantly, induction of IL-22Ra1 significantly increased IL-22 signaling as evidenced by pSTAT3 levels following IL-22 treatment.
[h=4]CONCLUSION:[/h] Collectively, these data suggest epithelial cells may optimize the beneficial effects of IL-22 through the induction of the IL-22 receptor during viral infection in the lung.
[h=4]KEYWORDS:[/h] IFNβ; IL-22; IL-22Ra1; Influenza; STAT1; TLR3
PMID: 31416461 PMCID: PMC6694528 DOI: 10.1186/s12931-019-1153-4
Free PMC Article