tetano
Editor, Senior Moderator
Vaccine. 2015 Sep 24. pii: S0264-410X(15)01305-5. doi: 10.1016/j.vaccine.2015.09.029. [Epub ahead of print]
[h=1]Improved stability of recombinant hemagglutinin using a formulation containing sodium thioglycolate.[/h] Rhodes DG[SUP]1[/SUP], Holtz K[SUP]2[/SUP], Robinson P[SUP]2[/SUP], Wang K[SUP]2[/SUP], McPherson CE[SUP]2[/SUP], Cox MM[SUP]2[/SUP], Srivastava IK[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study was designed to improve the stability of liquid formulations of recombinant influenza hemagglutinin (rHA) and to understand the mechanism of early loss of potency for rHA. The potency of rHA derived from several influenza strains was determined using single radial immunodiffusion (SRID), and the structure of the rHA was characterized using SDS-PAGE and dynamic light scattering. rHA formed disulfide cross-linked multimers, and potency decreased during extended storage. To reduce disulfide-mediated cross-linking and early potency loss, rHA was formulated with sodium thioglycolate (STG) and citrate. Addition of 80mM STG and 55mM sodium citrate inhibited disulfide-mediated cross-linking without affecting protein function for each rHA tested. The shelf life of the rHA formulation with STG-citrate, based on potency as determined by SRID, was extended as much as 20-fold, compared to a control formulation without STG-citrate. STG-citrate did not have a significant effect on the immunogenicity of H1 A/California/7/2009 rHA in mice.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza vaccine; SRID; STG; Stability; rHA
PMID: 26409814 [PubMed - as supplied by publisher]
[h=1]Improved stability of recombinant hemagglutinin using a formulation containing sodium thioglycolate.[/h] Rhodes DG[SUP]1[/SUP], Holtz K[SUP]2[/SUP], Robinson P[SUP]2[/SUP], Wang K[SUP]2[/SUP], McPherson CE[SUP]2[/SUP], Cox MM[SUP]2[/SUP], Srivastava IK[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study was designed to improve the stability of liquid formulations of recombinant influenza hemagglutinin (rHA) and to understand the mechanism of early loss of potency for rHA. The potency of rHA derived from several influenza strains was determined using single radial immunodiffusion (SRID), and the structure of the rHA was characterized using SDS-PAGE and dynamic light scattering. rHA formed disulfide cross-linked multimers, and potency decreased during extended storage. To reduce disulfide-mediated cross-linking and early potency loss, rHA was formulated with sodium thioglycolate (STG) and citrate. Addition of 80mM STG and 55mM sodium citrate inhibited disulfide-mediated cross-linking without affecting protein function for each rHA tested. The shelf life of the rHA formulation with STG-citrate, based on potency as determined by SRID, was extended as much as 20-fold, compared to a control formulation without STG-citrate. STG-citrate did not have a significant effect on the immunogenicity of H1 A/California/7/2009 rHA in mice.
Copyright ? 2015. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza vaccine; SRID; STG; Stability; rHA
PMID: 26409814 [PubMed - as supplied by publisher]