tetano
Editor, Senior Moderator
J Clin Immunol. 2011 Dec 15. [Epub ahead of print]
Increased Pro-inflammatory Cytokine Production in Down syndrome Children Upon Stimulation with Live Influenza A Virus.
Broers CJ, Gemke RJ, Weijerman ME, van der Sluijs KF, van Furth AM.
Source
Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081, HV, Amsterdam, the Netherlands, cjm.broers@vumc.nl.
Abstract
PURPOSE:
Children with down syndrome (DS) have an increased susceptibility to infections, due to altered humoral and/or cellular immunity. The aim of this study was to determine the cytokine production in whole blood of children with DS upon stimulation with live influenza A virus.
METHODS:
Whole blood of 61 children with DS and 57 of their healthy siblings was stimulated with 2.5 ? 10(4) TCID50/ml influenza A virus during 6, 24, and 48 h. TNF-α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IFN-α, IFN-γ concentrations, and viral load were measured at all time points.
RESULTS:
At most of the time points, TNF-α, IL-1β, IL-6, and IL-8 concentrations were significantly higher in children with DS following stimulation with live influenza A virus. IFN-α and IFN-γ levels were also significantly higher in the DS group. Viral clearance, however, was equal in both groups.
CONCLUSIONS:
Children with DS have an altered immune response to influenza A virus. The production of higher levels of pro-inflammatory cytokines may be responsible for a more severe clinical course of viral disease in these children.
PMID:
22170315
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22170315
Increased Pro-inflammatory Cytokine Production in Down syndrome Children Upon Stimulation with Live Influenza A Virus.
Broers CJ, Gemke RJ, Weijerman ME, van der Sluijs KF, van Furth AM.
Source
Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081, HV, Amsterdam, the Netherlands, cjm.broers@vumc.nl.
Abstract
PURPOSE:
Children with down syndrome (DS) have an increased susceptibility to infections, due to altered humoral and/or cellular immunity. The aim of this study was to determine the cytokine production in whole blood of children with DS upon stimulation with live influenza A virus.
METHODS:
Whole blood of 61 children with DS and 57 of their healthy siblings was stimulated with 2.5 ? 10(4) TCID50/ml influenza A virus during 6, 24, and 48 h. TNF-α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IFN-α, IFN-γ concentrations, and viral load were measured at all time points.
RESULTS:
At most of the time points, TNF-α, IL-1β, IL-6, and IL-8 concentrations were significantly higher in children with DS following stimulation with live influenza A virus. IFN-α and IFN-γ levels were also significantly higher in the DS group. Viral clearance, however, was equal in both groups.
CONCLUSIONS:
Children with DS have an altered immune response to influenza A virus. The production of higher levels of pro-inflammatory cytokines may be responsible for a more severe clinical course of viral disease in these children.
PMID:
22170315
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22170315