tetano
Editor, Senior Moderator
Vaccine. 2014 Aug 26. pii: S0264-410X(14)01153-0. doi: 10.1016/j.vaccine.2014.08.030. [Epub ahead of print]
Influenza M2 virus-like particles confer a broader range of cross protection to the strain-specific pre-existing immunity.
Kim MC1, Lee YN2, Hwang HS2, Lee YT2, Ko EJ2, Jung YJ2, Cho MK2, Kim YJ2, Lee JS2, Ha SH3, Kang SM4.
Author information
Abstract
Immunity in humans with annual vaccination does not provide effective protection against antigenically distinct strains. As an approach to improve cross-protection in the presence of pre-existing strain-specific immunity, we investigated the efficacy of heterologous and heterosubtypic protection in previously vaccinated mice at earlier times after subsequent immunization with conserved-antigenic target influenza M2 ectodomain (M2e) virus-like particle vaccine (M2e5? VLP). Immunization of mice with H1N1 split vaccine induced virus specific antibodies to homologous influenza virus but did not provide heterosubtypic hemagglutination inhibiting antibody responses and cross-protection. However, subsequent M2e5? VLP immunization induced M2e specific antibody response as well as interferon-γ (IFN-γ) producing cells in systemic and mucosal sites. Upon lethal challenge with H3N2 or H5N1 subtype influenza viruses, subsequently immunized mice with M2e5? VLP were well protected against heterosubtypic influenza viruses. These results provide evidence that non-seasonal immunization with M2e5? VLP, an experimental candidate for universal vaccine, is a promising approach for broadening the cross-protection even in the presence of strain-specific immunity.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Cross protection; Influenza vaccine; M2e5? VLPs; Pre-existing immunity
PMID:
25171841
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25171841
Influenza M2 virus-like particles confer a broader range of cross protection to the strain-specific pre-existing immunity.
Kim MC1, Lee YN2, Hwang HS2, Lee YT2, Ko EJ2, Jung YJ2, Cho MK2, Kim YJ2, Lee JS2, Ha SH3, Kang SM4.
Author information
Abstract
Immunity in humans with annual vaccination does not provide effective protection against antigenically distinct strains. As an approach to improve cross-protection in the presence of pre-existing strain-specific immunity, we investigated the efficacy of heterologous and heterosubtypic protection in previously vaccinated mice at earlier times after subsequent immunization with conserved-antigenic target influenza M2 ectodomain (M2e) virus-like particle vaccine (M2e5? VLP). Immunization of mice with H1N1 split vaccine induced virus specific antibodies to homologous influenza virus but did not provide heterosubtypic hemagglutination inhibiting antibody responses and cross-protection. However, subsequent M2e5? VLP immunization induced M2e specific antibody response as well as interferon-γ (IFN-γ) producing cells in systemic and mucosal sites. Upon lethal challenge with H3N2 or H5N1 subtype influenza viruses, subsequently immunized mice with M2e5? VLP were well protected against heterosubtypic influenza viruses. These results provide evidence that non-seasonal immunization with M2e5? VLP, an experimental candidate for universal vaccine, is a promising approach for broadening the cross-protection even in the presence of strain-specific immunity.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Cross protection; Influenza vaccine; M2e5? VLPs; Pre-existing immunity
PMID:
25171841
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25171841