tetano
Editor, Senior Moderator
Methods Mol Biol. 2019;1960:191-205. doi: 10.1007/978-1-4939-9167-9_17.
[h=1]Influenza-Mediated Lung Infection Models.[/h] McGee CE[SUP]1[/SUP], Sample CJ[SUP]1[/SUP], Kilburg-Basnyat B[SUP]2[/SUP], Gabor KA[SUP]3[/SUP], Fessler MB[SUP]3[/SUP], Gowdy KM[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter describes the technique of intranasal inoculation of male C57BL/6J mice with an H1N1 strain of influenza (A/Puerto Rico/8/1934) and methods for assessing the optimum dose for infection, viral titers in lung tissue, and severity of disease.
[h=4]KEYWORDS:[/h] Body weight loss; Focus-forming units; H1N1; Influenza; Intranasal inoculation; Lung damage
PMID: 30798533 DOI: 10.1007/978-1-4939-9167-9_17
[h=1]Influenza-Mediated Lung Infection Models.[/h] McGee CE[SUP]1[/SUP], Sample CJ[SUP]1[/SUP], Kilburg-Basnyat B[SUP]2[/SUP], Gabor KA[SUP]3[/SUP], Fessler MB[SUP]3[/SUP], Gowdy KM[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter describes the technique of intranasal inoculation of male C57BL/6J mice with an H1N1 strain of influenza (A/Puerto Rico/8/1934) and methods for assessing the optimum dose for infection, viral titers in lung tissue, and severity of disease.
[h=4]KEYWORDS:[/h] Body weight loss; Focus-forming units; H1N1; Influenza; Intranasal inoculation; Lung damage
PMID: 30798533 DOI: 10.1007/978-1-4939-9167-9_17